Genetic and Epidemiologic Trends of Norovirus Outbreaks in the United States from 2013 to 2016 Demonstrated Emergence of Novel GII.4 Recombinant Viruses.
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...*Dual genotyping based on sequencing partial RdRp and capsid regions (26,27)....
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...9) *Dual genotyping based on sequencing partial RdRp and capsid regions (26,27)....
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Cites methods from "Genetic and Epidemiologic Trends of..."
...REAGENT or RESOURCE SOURCE IDENTIFIER n-Acetyl-L-cysteine Sigma-Aldrich Cat# A9165-5G [Leu15]-Gastrin Sigma-Aldrich Cat# G9145 Noggin media Ettayebi et al 2016 N/A A83-01 Tocris Cat# 2939 DPBS, no calcium, no magnesium Invitrogen Cat# 14190-250 Sodium glycochenodeoxycholate Sigma-Aldrich Cat# G0759-100MG Collagen from human placenta (collagen type IV) Sigma-Aldrich Cat# C5533-5MG C2 Ceramide Santa Cruz Biotechnology Cat# sc-201375A 5 M Ethylenediaminetetraacetic (EDTA) Invitrogen Cat# 15575-020 0.05% Trypsin/0.5mM EDTA Gibco Cat# 25300054 Fetal bovine serum certified Life Technologies Cat# 16000-044 cOmplete, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat# 5056489001 Lysozyme from chicken egg white Sigma-Aldrich Cat# L6876 cOmplete His-Tag Purification Resin Sigma-Aldrich Cat# 5893801001 HRV-3C Protease Sigma-Aldrich Cat# SAE0045 Turbo293 Transfection Reagent Speed Biosystems Cat# PXX1002 AbBooster, Antibody Expression Enhancer Abi Scientific Cat# PB2668 Expi293 Expression Medium Thermo Fisher Scientific Cat# A1435101 GE MabSelect Protein A Resin Sigma-Aldrich Cat# GE17-5199-01 Pierce IgG Elution Buffer Thermo Fisher Scientific Cat# 21004 Polyethylene Glycol 4000 Rigaku Cat# 1008059 Tris-HCl buffer Rigaku Cat# HR2-237 0.1 M L-Proline Hampton Research Cat# HR2-428-26 Polyethylene Glycol 8000 Rigaku Cat# 1008062 CHES/Sodium Hydroxide Rigaku Cat#1008158 Critical Commercial Assays mMESSAGE MACHINE T7 Transcription kit Invitrogen Cat# AM1344 Deoxyribonuclease I Sigma Aldrich Cat# D4513-1VL TRIzol Thermo Fisher Scientific Cat# 15596-026 Rneasy Kit Qiagen Cat# 74004 SuperScript II Invitrogen Cat# 11904-018 Human Memory B Cell Isolation Kit Miltenyi Cat# 130-094-350 Oligo d(T)25 magnetic beads NEB Cat# S1419S One step fast qRT-PCR solution VWR Cat# 95039-940 N-hydroxysuccinimide (NHS)-agarose beads Thermo Fisher Scientific Cat# 26197 Melon gel purification Thermo Fisher Scientific Cat# 45212 Tris(2-carboxyethyl)phosphine Thermo Fisher Scientific Cat# 77720 Iodoacetamide Sigma Aldrich Cat# I1149-5G Trifluoroethanol Thermo Fisher Scientific Cat# AC13975-1000 Trypsin Gold Thermo Fisher Scientific Cat# PR-V5280 5x MagMAX - 96 Viral RNA Isolation Kit Applied Biosystems Cat# AMB1836-5 AgPath-ID One-Step RT-PCR Applied Biosystems Cat# 4387391 Deposited Data Crystal structure of GII.4.2002 P domain in complex with A1227 Fab This paper PDB: 6N81 Crystal structure of GII.4.2002 P domain in complex with A1431 Fab This paper PDB: 6N8D GII.4c Virus like particles This study GenBank: MK614455 (Continued on next page) Immunity 50, 1530–1541.e1–e8, June 18, 2019 e2...
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...Norovirus RNAwas detected by GI/GII TaqMan real-time RT-PCR (Cannon et al., 2017)....
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"Genetic and Epidemiologic Trends of..." refers methods in this paper
...The MEGA maximum likelihood model selection tool was used to determine the best model for branch support using the Bayesian information criterion for analysis, which was determined to be the Kimura two-parameter method with rate variation among sites modeled with a gamma distribution (K2 G) (76)....
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