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Journal ArticleDOI

Genetic characterization of Colletotrichum gloeosporioides isolates obtained from mango

01 Jan 1994-International Journal of Pest Management (Taylor & Francis Group)-Vol. 40, Iss: 2, pp 225-229
TL;DR: Most isolates of Colletotrichum gloeosporioides obtained from anthracnose lesions on mango leaves, inflorescences and fruits from different regions in Sri Lanka showed the same ribosomal DNA (rDNA) and very similar mitochondrial DNA (mtDNA) restriction fragment banding patterns when genomic DNA was digested with endonuclease EcoR I.
Abstract: Most isolates of Colletotrichum gloeosporioides (>80%) obtained from anthracnose lesions on mango leaves, inflorescences and fruits from different regions in Sri Lanka showed the same ribosomal DNA (rDNA) and very similar mitochondrial DNA (mtDNA) restriction fragment banding patterns when genomic DNA was digested with endonuclease EcoR I This rDNA restriction fragment banding pattern was the same as that revealed by C gloeosporioides isolates from mango in other tropical countries Isolates obtained from inflorescences all showed the same rDNA and mtDNA restriction patterns but a few isolates from fruits and leaves from mango grown in mixed orchards in areas of Sri Lanka with two monsoons and year‐round rainfall had rDNA restriction patterns associated with isolates from other fruit crops This suggested that some cross‐infection onto mango occurs However, it is suggested that there is a strain of C gloeosporioides specific to mango and the clonal nature of this strain implies that it has di
Citations
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Journal ArticleDOI
TL;DR: The limit of the Colletotrichum gloeosporioides species complex is defined genetically, based on a strongly supported clade within the Col letteredum ITS gene tree, as well as all taxa accepted within this clade, as it has been applied in the literature for the past 50 years.

905 citations


Cites background from "Genetic characterization of Colleto..."

  • ...…papers have reported genetically uniform populations of C. gloeosporioides associated with M. indica around the world (e.g. Hodson et al. 1993, Alahakoon et al. 1994, Sanders & Korsten 2003) and these perhaps also represent C. asianum, although DNA sequences are not available to confirm…...

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Journal ArticleDOI
TL;DR: Results are not inconsistent with the hypothesis that C. gloeosporioides isolates obtained from strawberry and non Cultivated hosts adjacent to strawberry fields are from the same population and that noncultivated hosts can serve as potential inoculum sources for Colletotrichum crown rot of strawberry.
Abstract: Colletotrichum crown rot of strawberry in Florida is caused primarily by Colletotrichum gloeosporioides To determine potential inoculum sources, isolates of Colletotrichum spp from strawberry and various noncultivated plants growing in the areas adjacent to strawberry fields were collected from different sites Species-specific internal transcribed spacer primers for C gloeosporioides and C acutatum were used to identify isolates to species Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among isolates recovered from noncultivated hosts and diseased strawberry plants Selected isolates also were tested for pathogenicity on strawberry plants in the greenhouse In all, 39 C gloeosporioides and 3 C acutatum isolates were recovered from diseased strawberry crowns, and 52 C gloeosporioides and 1 C acutatum isolate were recovered from noncultivated hosts In crown inoculation tests, 18 of the 52 C gloeosporioides isolates recovered from noncultivat

88 citations


Cites background from "Genetic characterization of Colleto..."

  • ...gloeosporioides from mango in Sri Lanka also revealed a greater amount of diversity in rDNA and mtDNA restriction fragment length polymorphisms than was previously thought to exist in this population (2)....

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Journal ArticleDOI
TL;DR: A wide range of anthracnose lesions was induced by the Mexican isolates in interactions with the various Stylosanthes genotypes, and a taxonomic infraspecific differentiation was proposed.

25 citations

Journal ArticleDOI
TL;DR: Analysis of sequence data of ITS and -tubulin regions of representative isolates did not group the lychee isolates into a monophyletic clade; however, given the majority of the isolates were from one of three genotypes found using ap-PCR, the possibility of a Lychee specific group of C.gloeosporioides is discussed.
Abstract: Since the 1980s a new disease has been affecting Australian lychee. Pepper spot appears as small, black superficial lesions on fruit, leaves, petioles and pedicels and is caused by Colletotrichum gloeosporioides, the same fungus that causes postharvest anthracnose of lychee fruit. The aim of this study was to determine if a new genotype of C.gloeosporioides is responsible for the pepper spot symptom. Morphological assessments, arbitrarily-primed PCR (ap-PCR) and DNA sequencing studies did not differentiate isolates of C.gloeosporioides from anthracnose and pepper spot lesions. The ap-PCR identified 21 different genotypes of C.gloeosporioides, three of which were predominant. A specific genotype identified using ap-PCR was associated with the production of the teleomorph in culture. Analysis of sequence data of ITS and -tubulin regions of representative isolates did not group the lychee isolates into a monophyletic clade; however, given the majority of the isolates were from one of three genotypes found using ap-PCR, the possibility of a lychee specific group of C.gloeosporioides is discussed.

23 citations

Journal Article
TL;DR: Estos resultados podrian indicar that the colonizacion endofitica es una importante ruta para el desarrollo de enfermedades en el mango ‘Haden’ cultivado en la Planicie de Maracaibo.
Abstract: Se evaluo la presencia y distribucion de hongos endofitos (asintomaticos) en cuatro plantaciones de mango ”˜Haden”™ sometidos a diferentes practicas agronomicas y ubicados en la Planicie de Maracaibo (condiciones semi-aridas). Los hongos se recuperaron empleando la tecnica de la triple esterilizacion (metodo especifico para hongos endofitos). Todos los hongos identificados son conocidos fitopatogenos del mango. En todas las plantaciones se registro la presencia de Fusarium decemcellulare, Lasiodiplodia theobromae, Colletotrichum gloesporioides, Alternaria alternata, Phomopsis mangiferae, Pestalotiopsis sp. y Cladosporium sp. La mayoria de los hongos estaban presentes tanto en organos vegetativos como reproductivos, con excepcion de P. mangiferae y Pestalotiopsis sp. que solo se recuperaron a partir de organos vegetativos. Conidios de L. theobromae y Cladosporium sp. fueron detectados dentro de las anteras junto con los granos de polen. En general, se encontro que la distribucion de los hongos en los organos de las plantas hospedantes fue continua y sistematica, sin registrarse variaciones temporales. Estos resultados podrian indicar que la colonizacion endofitica es una importante ruta para el desarrollo de enfermedades en el mango ”˜Haden”™ cultivado en la Planicie de Maracaibo.

18 citations


Cites background from "Genetic characterization of Colleto..."

  • ...Datos genéticos y geográficos sugieren que este patógeno se diseminó a través de las poblaciones de mango del mundo a partir de una sola fuente u origen por la vía endofítica (2)....

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References
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Book
15 Jan 2001
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Abstract: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Mol

215,169 citations

Journal ArticleDOI
TL;DR: A general, simple and inexpensive method for the isolation of DNA from filamentous fungi, starting from freeze‐dried mycelium 01–015% by weight, which allows the processing of many samples in parallel.
Abstract: We describe a general, simple and inexpensive method for the isolation of DNA from filamentous fungi. Starting from freeze-dried mycelium 01–015% by weight can be isolated as high molecular weight DNA suitable for restriction and ligation in 2 h. The preparation can be done in Eppendorf tubes and allows the processing of many samples in parallel. We have used the method with the basidiomycetes Phanerochaete chrysosporium, Coprinus cinereus and the ascomycete Aspergillus nidulans and others have used it with Trichoderma reesei, Aspergillus niger and for the isolation of DNA from tomato plants.

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Book
01 Jan 1992
TL;DR: The genus glomerella and its anamorph colletotrichum, B.C.Dillard coffee berry disease - the current status, Dinah Masaba and J.M.J. Sutton sexuality and genetics fo collett richum, R.A. Redman lectin cytochemistry - a new approach to understanding cell differentiation, pathogenesis and taxonomy in colletOTrichum.
Abstract: The genus glomerella and its anamorph colletotrichum, B.C. Sutton sexuality and genetics fo colletotrichum, R.J. Bryson, et al molecular transformation and genome analysis fo colletotrichum, R.J. Rodriquez and R.S. Redman lectin cytochemistry - a new approach to understanding cell differentiation, pathogenesis and taxonomy in colletotrichum, R.J. O'Connell, et al infection strategies of colletotrichum species, J.A. Bailey, et al mechanisms of resistance to colletotrichum species, M.T. Esquerre-Tugaye, et al colletotrichum diseases of legumes, Jillian M. Lenne colletotrichum diseases of perennial and other cash crops, J.M. Waller colletotrichum graminicola and the anthracnose diseases of maize and sorghum, Ralph L. Nicholson colletotrichum lindemuthianum on bean - population dynamics of the pathogen and breeding for resistance, J.C. Tu colletotrichum coccodes - the pathogen and its hosts, Helene R. Dillard coffee berry disease - the current status, Dinah Masaba and J.M. Waller molecular analysis of colletotrichum gloeosporioides pathogenic on the tropical pasture legume stylosanthes, J.M. Manners, et al molecular differentiation of colletotrichum gloesporioides isolates infecting tropical fruits, P.R. Mills, et al quiescent infections of colletotrichum in tropical and sub-tropical fruites, D. Prusky and R.A. Plumbley epidemiology of colletotrichum gloeosporioides in the tropics, J.C. Dodd, et al chemical control of colletotrichum infection in mangoes, C. Prior, et al strategies and prospects for biological contro of diseases caused by colletotrichum, P. Jeffries and I. Koomen use of colletotrichum strains as mycoherbidices, George E. Templeton.

470 citations

Journal ArticleDOI
TL;DR: Article de synthese comprenant: Nomenclature taxonomique; Symptomes, spectre d'hotes and processus d'infection; Physiologie de la quiescence; Epidemiologies de l'anthracnose; Methodes de lutte.
Abstract: Article de synthese comprenant: Nomenclature taxonomique; Symptomes, spectre d'hotes et processus d'infection; Physiologie de la quiescence; Epidemiologie de l'anthracnose; Methodes de lutte. On aborde plus specialement le cas du manguier, de l'avocatier, du bananier et du papayer

257 citations

Journal ArticleDOI
TL;DR: The procedure has been used successfully with several dozen isolates of the plant pathogenic fungus Cochliobolus heterostrophus (including both laboratory strains and isolates collected directly from the field), and has been found to be equally suitable for C. carbonum, Neurospora crassa, N. tetrasperma, and Nectria haematococca.

231 citations

Trending Questions (1)
Can Mango increase platelets?

This suggested that some cross‐infection onto mango occurs.