Genetic diversity in Sargasso Sea bacterioplankton.
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Cites background or methods from "Genetic diversity in Sargasso Sea b..."
...To assess this diversity, PCR amplification of 16S rDNA (13) and cloning and sequence analysis of selected clones have been applied previously (9)....
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...They constitute the cloning of ribosomal copy DNA (21) or polymerase chain reaction (PCR)amplified ribosomal DNA (rDNA) (9) followed by sequence analysis of the resulting clones....
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...bial communities and to identifying several uncultured microorganisms (9, 21)....
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Cites background from "Genetic diversity in Sargasso Sea b..."
...Since the observation that culture-based methods of microbiota analysis were inadequate, microbiologists have turned to the molecular-based techniques traditionally used in microbial ecology to characterize complex marine and soil communities (90, 137)....
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2,383 citations
Cites background from "Genetic diversity in Sargasso Sea b..."
...999%) of the organisms existing in nature appear to be refractory to cultivation (1, 3, 11, 30, 35)....
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References
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