Journal ArticleDOI
GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode.
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TLDR
The fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number is correlated, extending the use of fluorescent proteins to applications and assays where cell growth rates are important.Abstract:
This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell growth rates are important. Using a standard fluorometer, growth of these cells can be quantified noninvasively in multiwell plates, and because signals are obtained without preparation, the same culture samples can be measured repeatedly. Even with a small relative change in biomass, the specific growth rate can be determined in a few hours. The dynamics of cell populations can now be studied with high sensitivity, low error rate, and minimum sample preparation.read more
Citations
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Synthesis, biological evaluation and molecular docking of novel chalcone-coumarin hybrids as anticancer and antimalarial agents.
Ratchanok Pingaew,Amporn Saekee,Prasit Mandi,Chanin Nantasenamat,Supaluk Prachayasittikul,Somsak Ruchirawat,Virapong Prachayasittikul +6 more
TL;DR: Molecular docking suggested that the cytotoxicity of reported hybrids could be possibly due to their dual inhibition of α- and β-tubulins at GTP and colchicine binding sites, respectively, and falcipain-2 was identified to be a plausible target site of the hybrids given their antimalarial potency.
Journal ArticleDOI
Biotechnological applications of green fluorescent protein
TL;DR: A brief compendium of GFP′s contributions to biotechnology is provided: Protein fusions, transcriptional reporters, whole-organism visualization, and various other screening applications are reviewed with respect to biotechnological applications.
Journal ArticleDOI
Small-scale bioreactor system for process development and optimization
TL;DR: An agitated 12-well microtiter plate system with a working volume of 2ml was investigated for cell culture process development, which assures homogeneity in wells and enhances mass transfer between the gas and the liquid phase, thus improving maximum cell density and pH stability.
Journal ArticleDOI
Claurailas A-D, cytotoxic carbazole alkaloids from the roots of Clausena harmandiana.
TL;DR: Four new carbazole alkaloids, claurailas A-D (1-4), as well as 12 known carbazoles and three known coumarins were isolated from the roots of Clausena harmandiana, showing strong cytotoxicity against NCI-H187 and KB cell lines.
Journal Article
Cell-Based Assays in High-Throughput Screening for Drug Discovery
TL;DR: The uses of different types of cells and cell culture systems, including 2D, 3D and perfusion cell cultures, in cell-based HTS for drug discovery are discussed and potential applications for screening phytochemicals and herbal medicines are provided.
References
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
Green fluorescent protein as a marker for gene expression
TL;DR: A complementary DNA for the Aequorea victoria green fluorescent protein produces a fluorescent product when expressed in prokaryotic or eukaryotic cells, which can be used to monitor gene expression and protein localization in living organisms.
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Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
TL;DR: It was shown that phenol red does not interfere with the measurements and no washing steps are required since all ingredients can be added subsequently, and Serum proteins at concentrations up to 25% have no influence on the result.
PatentDOI
FACS-optimized mutants of the green fluorescent protein (GFP)
TL;DR: In this article, three classes of GFP mutants having single excitation maxima around 488 nm are shown to be brighter than wild-type GFP following 488-nm excitation.
Journal ArticleDOI
Primary structure of the Aequorea victoria green-fluorescent protein.
Douglas Prasher,Virginia K. Eckenrode,William W. Ward,Frank G. Prendergast,Milton J. Cormier +4 more
TL;DR: The cloning and sequencing of both cDNA and genomic clones of GFP from the cnidarian, Aequorea victoria, show three different restriction enzyme patterns which suggests that at least three different genes are present in the A. victoria population.