Global Epidemiology of Campylobacter Infection
01 Jul 2015-Clinical Microbiology Reviews (American Society for Microbiology)-Vol. 28, Iss: 3, pp 687-720
TL;DR: Overall, campylobacteriosis is still one of the most important infectious diseases that is likely to challenge global health in the years to come.
Abstract: Campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. The incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. The dramatic increase in North America, Europe, and Australia is alarming, and data from parts of Africa, Asia, and the Middle East indicate that campylobacteriosis is endemic in these areas, especially in children. In addition to C. jejuni, there is increasing recognition of the clinical importance of emerging Campylobacter species, including Campylobacter concisus and Campylobacter ureolyticus. Poultry is a major reservoir and source of transmission of campylobacteriosis to humans. Other risk factors include consumption of animal products and water, contact with animals, and international travel. Strategic implementation of multifaceted biocontrol measures to reduce the transmission of this group of pathogens is paramount for public health. Overall, campylobacteriosis is still one of the most important infectious diseases that is likely to challenge global health in the years to come. This review provides a comprehensive overview of the global epidemiology, transmission, and clinical relevance of Campylobacter infection.
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TL;DR: Chlorous acid could be a better disinfectant in chicken slaughtering and processing to kill campylobacters and prevent contamination and the bactericidal mechanisms of chlorous acid may be due to damages of bacterial proteins.
8 citations
TL;DR: RAA-exo-probe and RAA-CRIPSR/Cas12a assays were found to be highly specific to C. jejuni and highly sensitive, as they were one log more sensitive compared to the traditional culture method, with detection thresholds of 9 and 5 copies per reaction, respectively.
Abstract: Campylobacter jejuni is the major cause of campylobacteriosis, one of the most common foodborne illnesses worldwide. Here, we report the development of RAA-exo-probe and RAA-CRIPSR/Cas12a assays for the detection of C. jejuni in food samples. The two assays were found to be highly specific to C. jejuni and highly sensitive, as they were one log more sensitive compared to the traditional culture method, with detection thresholds of 9 and 5 copies per reaction, respectively. These assays successfully detected C. jejuni in spiked chicken samples and natural meat samples (chicken, beef, mutton, etc.) and were overall less dependent on expensive equipment, only requiring a fluorescent reader. Their ease of use compared to other nucleic acid amplification-based methods indicates that these assays could be adapted for the rapid, routine surveillance of C. jejuni contamination in food samples, particularly for work done in the field or poorly equipped labs.
8 citations
TL;DR: In this paper, the concept of Innolysin composed of an endolysin fused to a phage receptor binding protein (RBP) was extended to target Campylobacter jejuni.
Abstract: Campylobacter contaminated poultry remains the major cause of foodborne gastroenteritis worldwide, calling for novel antibacterials. We previously developed the concept of Innolysin composed of an endolysin fused to a phage receptor binding protein (RBP) and provided the proof-of-concept that Innolysins exert bactericidal activity against Escherichia coli. Here, we have expanded the Innolysin concept to target Campylobacter jejuni. As no C. jejuni phage RBP had been identified so far, we first showed that the H-fiber originating from a CJIE1-like prophage of C. jejuni CAMSA2147 functions as a novel RBP. By fusing this H-fiber to phage T5 endolysin, we constructed Innolysins targeting C. jejuni (Innolysins Cj). Innolysin Cj1 exerts antibacterial activity against diverse C. jejuni strains after in vitro exposure for 45 min at 20°C, reaching up to 1.30 ± 0.21 log reduction in CAMSA2147 cell counts. Screening of a library of Innolysins Cj composed of distinct endolysins for growth inhibition, allowed us to select Innolysin Cj5 as an additional promising antibacterial candidate. Application of either Innolysin Cj1 or Innolysin Cj5 on chicken skin refrigerated to 5°C and contaminated with C. jejuni CAMSA2147 led to 1.63 ± 0.46 and 1.18 ± 0.10 log reduction of cells, respectively, confirming that Innolysins Cj can kill C. jejuni in situ. The receptor of Innolysins Cj remains to be identified, however, the RBP component (H-fiber) recognizes a novel receptor compared to lytic phages binding to capsular polysaccharide or flagella. Identification of other unexplored Campylobacter phage RBPs may further increase the repertoire of new Innolysins Cj targeting distinct receptors and working as antibacterials against Campylobacter.
8 citations
01 Jan 2017
TL;DR: This chapter reviews the methodologies developed to isolate, identify, and characterize C. jejuni and C. coli and emphasizes the most efficient methods for isolation of Campylobacter from foods, and for the typing of isolates for epidemiological studies.
Abstract: Campylobacter jejuni and C. coli are the bacterial species most commonly associated with foodborne illness worldwide. Methods for isolation of these organisms from complex food matrices rely on conditions that allow for the growth of these organisms while inhibiting competitors. This chapter reviews the methodologies developed to isolate, identify, and characterize C. jejuni and C. coli. Several of the unique requirements for growth have been incorporated in the different methodologies available for isolation of Campylobacter spp. from foods. The incorporation of DNA-based identification methods has improved the specificity and the speed at which results are now available. These DNA-based methods are also responsible for the rapid development of typing protocols with high resolution for epidemiological studies. This Chapter emphasizes the most efficient methods for isolation of Campylobacter from foods, and for the typing of isolates for epidemiological studies.
8 citations
01 Jan 2015
TL;DR: In this article, the authors compared the performance of wastewater and faecal sludge management and reuse systems in Kampala, Uganda, a low-income African city, and in Hanoi, Vietnam, a lower-middle-income Asian city.
Abstract: Background: Reuse and recovery of wastewater in agriculture and aquaculture has gained traction in the new millennium. In view of continued population growth, increasing scarcity of freshwater and other natural resources, the demand to boost food production and efforts to enhance wastewater reuse will increase in the years to come. Indeed, wastewater reuse and the recovery of water, nutrients and energy can generate promising business opportunities and support livelihoods in poor communities, particularly in urban and peri-urban areas of low- and middle-income countries (LMICs). Contact with untreated wastewater is associated with microbial and chemical hazards and thus might negatively affect human health. Standardised, quality-controlled methods to assess and manage health risks are available, such as those described in the World Health Organization (WHO) guidelines for the safe use of wastewater, excreta and greywater. However, the practicability and uptake of these methods have proved difficult in LMICs. There is a paucity of context-specific, quality-based environmental pollution data, epidemiological data and accurate disease burden estimates for highly dynamic environments along wastewater recovery and reuse systems in major urban settings, especially in Africa, Asia and Latin America. Moreover, discharge thresholds and health-based targets need to be reviewed to match the realities of LMICs.
Objectives: This PhD thesis aims to generate evidence of health risks among people living and working along wastewater and faecal sludge management and reuse systems in Kampala, Uganda, a low-income African city, and in Hanoi, Vietnam, a lower-middle-income Asian city. By comparing relevant conditions in these two systems, the thesis seeks to: (i) generate evidence on microbial and chemical contamination and treatment capacities along wastewater management systems; (ii) assess prevalence and risk factors of intestinal parasitic infections in different population groups exposed to wastewater and faecal sludge; (iii) estimate the burden of gastrointestinal infections due to the exposure to wastewater; and (iv) discuss and compare risk assessment approaches and their potential for application along wastewater recovery reuse systems in selected LMICs.
Research partnership: This PhD thesis is embedded in the “Resource Recovery and Reuse” (RRR) project, funded by the Swiss Agency for Development and Cooperation (SDC). Our main partner in this collaborating endeavour is WHO, while other international partners include the International Water Management Institute, the International Centre for Water Management Services, and the Department of Water and Sanitation in Developing Countries, Swiss Federal Institute of Aquatic Science and Technology. In Uganda, we closely work with the Makerere School of Public Health, the Vector Control Division of the Ministry of Health and the National Water and Sewerage Corporation. In Hanoi, our main partner is the Center for Public Health and Ecosystem Research at Hanoi School of Public…
8 citations
References
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University of Maryland, Baltimore1, Centers for Disease Control and Prevention2, International Centre for Diarrhoeal Disease Research, Bangladesh3, Aga Khan University4, Medical Research Council5, University of Barcelona6, GlaxoSmithKline7, United States Department of Veterans Affairs8, Center for Drug Evaluation and Research9, University of Bergen10, Norwegian Institute of Public Health11, Royal Children's Hospital12
TL;DR: Interventions targeting five pathogens can substantially reduce the burden of moderate-to-severe diarrhoea and suggest new methods and accelerated implementation of existing interventions (rotavirus vaccine and zinc) are needed to prevent disease and improve outcomes.
2,766 citations
"Global Epidemiology of Campylobacte..." refers background in this paper
...with moderate to severe diarrhea in children from Kolkata, India, Mirzapur, Bangladesh, and Karachi, Pakistan (66)....
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...In addition, in a prospective case-control study conducted between 1 December 2007 and 3 March 2011 to identify the etiology of diarrhea in children aged 0 to 59 months, C. jejuni was reported to be significantly associated July 2015 Volume 28 Number 3 cmr.asm.org 693Clinical Microbiology Reviews on M arch 21, 2021 by guest http://cm r.asm .org/ D ow nloaded from with moderate to severe diarrhea in children from Kolkata, India, Mirzapur, Bangladesh, and Karachi, Pakistan (66)....
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TL;DR: Two important processes have occurred to legitimize these conditions, and to increase attention toward the research and clinical care of patients with functional gastrointestinal disorders (FGID), a shift in conceptualizing these disorders from a disease-based, reductionistic model, to a more integrated, biopsychosocial model of illness.
2,274 citations
TL;DR: Overabundance of Fusobacterium sequences in tumor versus matched normal control tissue is verified by quantitative PCR analysis from a total of 99 subjects, and a positive association with lymph node metastasis is observed.
Abstract: An estimated 15% or more of the cancer burden worldwide is attributable to known infectious agents. We screened colorectal carcinoma and matched normal tissue specimens using RNA-seq followed by host sequence subtraction and found marked over-representation of Fusobacterium nucleatum sequences in tumors relative to control specimens. F. nucleatum is an invasive anaerobe that has been linked previously to periodontitis and appendicitis, but not to cancer. Fusobacteria are rare constituents of the fecal microbiota, but have been cultured previously from biopsies of inflamed gut mucosa. We obtained a Fusobacterium isolate from a frozen tumor specimen; this showed highest sequence similarity to a known gut mucosa isolate and was confirmed to be invasive. We verified overabundance of Fusobacterium sequences in tumor versus matched normal control tissue by quantitative PCR analysis from a total of 99 subjects (p = 2.5 × 10(-6)), and we observed a positive association with lymph node metastasis.
1,535 citations
TL;DR: The composition of the microbiota in colorectal carcinoma is characterized using whole genome sequences from nine tumor/normal pairs and Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs.
Abstract: The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.
1,527 citations
TL;DR: Campylobacters are a relatively unrecognised cause of acute enteritis, but these findings suggest that they may be a common cause, and poultry may be the primary source of the organism.
Abstract: By selective culture campylobacters (C jejuni and C coli) were isolated from the faeces of 57 (7-1%) out of 803 unselected patients with diarrhoea; none were isolated from 194 people who had not got diarrhoea. Specific agglutinins were found in the sera of 31 out of 38 patients with campylobacter enteritis and 10 of them had a rising titre. Half the patients were aged 15 to 44 years, but the incidence was highest in young children. All the patients with campylobacters had a distinctive clinical illness with severe abdominal pain. Campylobacters are a relatively unrecognised cause of acute enteritis, but these findings suggest that they may be a common cause. Spread of infection was observed within 12 out of 29 households, and in these cases children were usually implicated. Several patients were apparently infected from chickens, both live and dressed, and poultry may be the primary source of the organism. In two cases dogs with diarrhoea were found to be infected with strains indistinguishable from their human contacts. Ten patients acquired their infections while travelling abroad.
1,431 citations