Journal ArticleDOI
Glutathione peroxidase activity in selenium-deficient rat liver☆
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TLDR
Two peaks of glutathione peroxidase activity were present in the Sephadex G-150 gel filtration chromatogram of rat liver supernatant when 1.5 mM cumene hydroperoxide was used as substrate, and the second peak represents a second glutathienase activity which catalyzes the destruction of organic hydroperoxides but has little activity toward H 2 O 2 and which persists in severe selenium deficiency.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1976-08-23. It has received 3181 citations till now. The article focuses on the topics: Cumene hydroperoxide & Selenium deficiency.read more
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Book ChapterDOI
[44] Glutathione peroxidase
TL;DR: This chapter presents a procedure for the preparation of glutathione peroxidase, which is regarded as a major protective system against endogenously and exogenously induced lipid peroxidation.
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Role of oxidative stress in cardiovascular diseases.
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Mice Deficient in Cellular Glutathione Peroxidase Show Increased Vulnerability to Malonate, 3-Nitropropionic Acid, and 1-Methyl-4-Phenyl-1,2,5,6-Tetrahydropyridine
Péter Klivényi,Ole A. Andreassen,Robert J. Ferrante,Alpaslan Dedeoglu,Gerald Mueller,Eric Lancelot,Mikhail B. Bogdanov,Julie K. Andersen,Dongmei Jiang,M. Flint Beal,M. Flint Beal +10 more
TL;DR: The present results indicate that a knock-out of GSHPx may be adequately compensated under nonstressed conditions, but that after administration of mitochondrial toxins GSHpx plays an important role in detoxifying increases in oxygen radicals.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase
TL;DR: Glutathione peroxidase activity is found to be associated with a relatively stable, nondialyzable, heat-labile, intracellular component which is separable from hemoglobin, by gel filtration and ammonium sulfate precipitation.
Journal ArticleDOI
Selenium: Biochemical Role as a Component of Glutathione Peroxidase
TL;DR: When hemolyzates from erythrocytes of selenium-deficient rats were incubated in vitro in the presence of ascorbate or H2O2, added glutathione failed to protect the hemoglobin from oxidative damage.
Journal ArticleDOI
Glutathione peroxidase: A selenoenzyme
TL;DR: The aulh,o~s described an incorpora t ion o f in t raper imne~ ly injee~ted '~s Se in to a IVrOtein f ract ion w N e h after partial puMfieafion showed GSH peroxidase a ctiVi:ty.
Journal ArticleDOI
Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat
TL;DR: The results suggest that tissue GSH-Px can be used as an indicator of animal Se status, but other factors such as age, sex, and dietary vitamin E may have to be considered.