Green synthesis of copper-doped nickel oxide nanoparticles using okra plant extract for the evaluation of their cytotoxicity and photocatalytic properties
TL;DR: In this paper, the authors have synthesized copper doping nickel oxide nanoparticles (Cu-doped NiO-NPs) by a sol-gel method that involved the application of okra plant extract and had investigated their photocatalytic properties and cytotoxicity effects.
About: This article is published in Ceramics International.The article was published on 2021-10-01. It has received 28 citations till now. The article focuses on the topics: Nanoparticle & Nickel oxide.
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TL;DR: In this article , the authors demonstrated a novel Cleome simplicifolia-mediated green fabrication of nickel oxide nanoparticles (NiO NPs) to explore in vitro toxicity in Bm-17 and Labeo rohita liver cells.
Abstract: The present work demonstrated a novel Cleome simplicifolia-mediated green fabrication of nickel oxide nanoparticles (NiO NPs) to explore in vitro toxicity in Bm-17 and Labeo rohita liver cells. As-fabricated bioinspired NiO NPs were characterized by several analytical techniques. X-ray diffraction (XRD) revealed a crystalline face-centered-cubic structure. Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible diffuse reflectance spectroscopy (UV-DRS), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS) confirmed NiO formation. The chemical composition was confirmed by energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy. Brunauer–Emmett–Teller (BET) revealed the mesoporous nature. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed the formation of 97 nm diameter nanospheres formed due to the congregation of 10 nm size particles. Atomic force microscopy (AFM) revealed the nearly isotropic behavior of NiO NPs. Further, a molecular docking study was performed to explore their toxicity by binding with genetic molecules, and it was found that the docking energy was about −9.65284 kcal/mol. On evaluating the in vitro toxicity of NiO NPs for Bm-17 cells, the study showed that when cells were treated with a high concentration of NPs, cells were affected severely by toxicity, while at a lower concentration, cells were affected slightly. Further, on using 50 μg/mL, quick deaths of cells were observed due to the formation of more vacuoles in the cells. The DNA degradation study revealed that NiO NPs are significantly responsible for DNA degradation. For further confirmation, trypan blue assay was observed for cell viability, and morphological assessment was performed using inverted tissue culture microscopy. Further, the cytotoxicity of NiO NPs in L. rohita liver cells was studied. No toxicity was observed at 1 mg/L of NiO NPs; however, when the concentration was 30 and 90 mg/L, dark and shrank hepatic parenchyma was observed. Hence, the main cause of cell lysis is the increased vacuolization in the cells. Thus, the present study suggests that the cytotoxicity induced by NiO NPs could be used in anticancer drugs.
21 citations
TL;DR: In this paper , the authors highlight the recent development and applications of biochars and/or green nanoparticles (NPs) produced from agricultural waste via green routes in removing the refractory pollutants from water and wastewater.
19 citations
01 Jan 2021
TL;DR: In this paper, a detailed report on eco-friendly methods for copper nanoparticles synthesis and also highlighted factors influencing the synthesis of Cu-NPs from plant sources is presented, and detailed benefits of phyto-synthesized CuNPs in biomedical field, agricultural section and environmental control are comprehensively discussed.
Abstract: The great interest attached to Copper nanoparticles (CuNPs) are traceable to its distinctive properties such as rigidity, high yield strength, high surface-to-volume ratio, hardness, flexibility and ductility. CuNPs have shown several applications such as catalytic, antiviral, antibacterial, anti-inflammatory, antioxidant, and antifungal activities. Recently, Cu-NPs has gain a lot of importance comparable with gold and silver in several fields, it also offer additional benefit of been cheaper. The biosynthesis of Cu-NPs has been recommended as a valuable substitute to both the physical and chemical methods because its ecofriendly, relative cheap and entails easy process. The synthesis of Cu-NPs from plant extracts had gained significant attention over the years and its massive contribution to numerous industries and institutes is alarming. This review present a detailed report on eco-friendly methods for Cu-NPs synthesis and also highlighted factors influencing the synthesis of Cu-NPs from plant sources. The characterization techniques needed for Cu-NPs analysis are well discussed. Detailed benefits of phyto-synthesized Cu-NPs in biomedical field, agricultural section and environmental control are comprehensively discussed.
15 citations
TL;DR: In this article , the green synthesis of silver nanoparticles (Ag NPs) using the methanolic extract of the aerial parts of Lavandula coronopifolia was confirmed through color change as well as the UV/visible spectra.
Abstract: The present study successfully investigated the green synthesis of silver nanoparticles (Ag NPs) using the methanolic extract of the aerial parts of Lavandula coronopifolia. The formation of Ag NPs was confirmed through color change as well as the UV/visible spectra. Further, characterizations were achieved using Fourier Transforms infrared spectroscopy (FT-IR), transmission electron microscope (TEM), thermal gravimetric analysis (TGA), and dynamic light scatting measurements (DLS). The collected isolates (twelve isolates) were identified microscopically as 8 Candida and 4 Aspergillus isolates. The Ag NPs exhibited excellent antifungal activity results against tested isolates compared with L. coronopifolia extract and the antifungal standard (Ketoconazole) with p < 0.01. Ag NPs showed highly significant results towards C. albicans, C. tropicalis, A. fumigatus, and A. niger with MICs ranges of 1.7–0.7 μg/ml (p < 0.01). Depending on the stability and the effective diameters of the prepared Ag NPs using L. coronopifolia extract, it could be used as a simple, green preparation, eco-friendly, and low costs process that can be applied to the veterinary sector.
10 citations
TL;DR: In this article , the authors used Murraya Koenigii leaf extracts to replace synthetic bactericides to synthesize NiO and Co-doped NiO nanoparticles using XRD, UV-Vis, FTIR, SEM, and EDAX analytical techniques.
Abstract: Recently, the synthesis of nanoparticles using phytoextracts has been focused worldwide due to their reduced chemical toxicity. We prepared NiO and Co-doped NiO nanoparticles using Murraya Koenigii leaf extracts to replace synthetic bactericides. The prepared samples were characterized by XRD, UV–Vis, FTIR, SEM, and EDAX analytical techniques. According to XRD analysis, the green synthesised NiO and Co-doped NiO nanoparticles have a crystalline cubic structure. NiO and Co-doped NiO nanoparticles showed a characteristic absorption peak at 348 nm. FTIR spectroscopy confirmed the functional groups of biomolecules present in the synthesised materials. The size of the prepared nanoparticles was found to be 80–250 nm as observed through SEM analysis. Enhanced antibacterial activity was observed for Co doped NiO against gram negative (Klebsiella pneumonia, Shigella dysenteriae ), gram positive ( Staphylococcus aureus , and Bacillus) bacterial strains. • Murraya Koenigii leaf extracts assisted green synthesis was adopted to prepare NiO and Co doped NiO nanoparticles. • The effect of Co doping on structural, optical and morphological properties of NiO nanoparticles were analysed. • The excellent antimicrobial activity of pure and doped NiO nanoparticles were reported.
9 citations
References
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TL;DR: This work used Drosophila melanogaster larvae to develop a high-throughput whole organism screen for drugs that modulate food intake and identified the serotonin (5-hydroxytryptamine or 5-HT) receptor antagonist metitepine as a potent anorectic drug.
Abstract: Dysregulation of eating behavior can lead to obesity, which affects 10% of the adult population worldwide and accounts for nearly 3 million deaths every year. Despite this burden on society, we currently lack effective pharmacological treatment options to regulate appetite. We used Drosophila melanogaster larvae to develop a high-throughput whole organism screen for drugs that modulate food intake. In a screen of 3630 small molecules, we identified the serotonin (5-hydroxytryptamine or 5-HT) receptor antagonist metitepine as a potent anorectic drug. Using cell-based assays we show that metitepine is an antagonist of all five Drosophila 5-HT receptors. We screened fly mutants for each of these receptors and found that serotonin receptor 5-HT2A is the sole molecular target for feeding inhibition by metitepine. These results highlight the conservation of molecular mechanisms controlling appetite and provide a method for unbiased whole-organism drug screens to identify novel drugs and molecular pathways modulating food intake.
2,329 citations
TL;DR: The localized surface plasmon resonance (LSPR) of oxide-free Cu nanoparticles fabricated by nanosphere lithography is examined by UV−vis extinction spectroscopy and electrodynamics theory.
Abstract: The localized surface plasmon resonance (LSPR) of oxide-free Cu nanoparticles fabricated by nanosphere lithography is examined by UV−vis extinction spectroscopy and electrodynamics theory. The LSPR of the Cu nanoparticles is significantly affected by the presence of copper oxides and the removal of the oxide species yields a dramatic difference in the observed LSPR. From a comparison of the LSPR of Cu, Ag, and Au nanoparticles of similar geometry, we conclude that Cu displays an intense and narrow LSPR peak that is comparable to Ag and Au.
767 citations
TL;DR: Novel sol-gel synthetic techniques were used to immobilize copper-zinc superoxide dismutase, cytochrome c, and myoglobin by encapsulation in stable, optically transparent, porous silica glass matrices under mild conditions such that the biomolecules retained their characteristic reactivities and spectroscopic properties.
Abstract: Novel sol-gel synthetic techniques were used to immobilize copper-zinc superoxide dismutase (CuZnSOD), cytochrome c, and myoglobin (Mb) by encapsulation in stable, optically transparent, porous silica glass matrices under mild conditions such that the biomolecules retained their characteristic reactivities and spectroscopic properties. The resulting glasses allowed transport of small molecules into and out of the glasses at reasonable rates but nevertheless retained the protein molecules within their pores. Chemical reactions of the immobilized proteins could be monitored by means of changes in their visible absorption spectra. Silica glasses containing the immobilized proteins were observed to have similar reactivities and spectroscopic properties to those found for the proteins in solution. For example, encapsulated CuZnSOD was demetallated and remetallated, encapsulated ferricytochrome c was reduced and then reoxidized, and encapsulated met Mb was reduced to deoxy Mb and then reacted either with dioxygen to make oxy Mb or with carbon monoxide to make carbonyl Mb.
687 citations
TL;DR: In this paper, the authors investigated the dielectric parameters of copper containing ferrites and found an abnormal behaviour of the constant and loss factor as a function of frequency and temperature in comparison with the normal behaviour of spinel ferrites.
Abstract: The dielectric parameters are investigated as a function of composition, frequency, and temperature in copper containing ferrites. An “abnormal” behaviour of the dielectric constant is found and also the loss factor as a function of frequency and temperature in comparison with the “normal” behaviour of spinel ferrites. The origin of this abnormal behaviour is attributed to the presence of Cu1+ ions which determine the appearance of p-carriers in these ferrites.
On a analyse le comportement des parametres dielectriques en fonction de la composition, la frequence et la temperature dans les ferrites qui contiennent du cuivre. On a trouve un comportement „anormal” de la constante dielectrique et du facteur de pertes dielectriques en fonction de la frequence et la temperature par rapport au comportement „normal” pour les ferrites de type spinelle. L'origine de ce comportement anormal est attribue a la presence des ions Cu1+ qui determinent l'apparition des porteurs de type p dans ces ferrites.
471 citations
TL;DR: A green chemistry method for synthesized colloidal silver nanoparticles (Ag NPs) in polymeric media using silver nitrate, polyethylene glycol, and β-D-glucose as a silver precursor, stabilizer, and reducing agent is reported.
Abstract: The roles of green chemistry in nanotechnology and nanoscience fields are very significant in the synthesis of diverse nanomaterials. Herein, we report a green chemistry method for synthesized colloidal silver nanoparticles (Ag NPs) in polymeric media. The colloidal Ag NPs were synthesized in an aqueous solution using silver nitrate, polyethylene glycol (PEG), and β-D-glucose as a silver precursor, stabilizer, and reducing agent, respectively. The properties of synthesized colloidal Ag NPs were studied at different reaction times. The ultraviolet-visible spectra were in excellent agreement with the obtained nanostructure studies performed by transmission electron microscopy (TEM) and their size distributions. The Ag NPs were characterized by utilizing X-ray diffraction (XRD), zeta potential measurements and Fourier transform infrared (FT-IR). The use of green chemistry reagents, such as glucose, provides green and economic features to this work.
434 citations