Green Synthesis of Silver Nanoparticle using Kalanchoe pinnata leaf extract and its Antibacterial Effect against Gram-Positive and Gram-Negative Species
30 Sep 2018-Research Journal of Pharmacy and Technology (A and V Publication)-Vol. 11, Iss: 9, pp 3964-3968
TL;DR: In this article, a rapid, non-toxic, eco-friendly procedure for the synthesis of silver nanoparticles (Ag NP) using Kalanchoe pinnata fresh leaves extract was presented.
Abstract: This study presents a rapid, non-toxic, eco- friendly procedure for the synthesis of silver nanoparticle (Ag NP) using Kalanchoe pinnata fresh leaves extract. The plant extract acted as a reducing agent as well as a capping and stabilization agent. Synthesis of nanoparticle was estimated by visual interpretation as a preliminary test and UV-visible Spectroscopy showing standard peak at the range of 430 nm as a confirmatory test. X-ray diffraction assay (XRD) confirmed the crystalline nature of synthesized nanoparticle. Fourier transform infrared spectroscopy (FT-IR) analysis was performed to identify the functional groups of plant involved in the reduction of metallic silver. Size estimation of nanoparticle was conducted through Scanning electron microscopy (SEM) analysis which confirmed the size range of nanoparticle as 50-70 nm. Further characterization of biosynthesized nanoparticle included Atomic force microscopy (AFM) imaging which depicted the topography of the nanoparticle. Elemental composition analysis of the nanoparticles was conducted using Energy dispersion analysis of X-ray (EDAX) analysis that demonstrated the presence of silver in great proportion. Potent antibacterial activity of green synthesized silver nanoparticle have been found against both Gram-positive species (Staphylococcus sp., Bacillus sp., Listeria sp., Salmonella sp.) and Gram-negative bacteria like E.coli sp.
Citations
More filters
TL;DR: The use of nanotechnology in medicine in particular, specifically drug delivery is shown to have various benefits as discussed by the authors, where nanoparticles are being used to reduce toxicity and side effects that drugs may impose to the patient.
17 citations
TL;DR: In this article, a review aimed at understanding the synthesis, mechanism, and applications of Ag NPs in dentistry and their qualities to aid clinicians and researchers was performed on the Google Scholar, PubMed, EMBASE, and Web of Science databases for related articles using multiple keywords.
Abstract: Background. The silver nanoparticles (Ag NPs) are the most acceptable and excellent nanotechnology-based product among all metallic nanoparticles (noble metals). They are novel in terms of good conductivity, catalytic, chemical stability, and most significant anti-inflammatory and antiviral activities. Aim. This review is aimed at understanding the synthesis, mechanism, and applications of Ag NPs in dentistry and their qualities to aid clinicians and researchers. Materials and Methods. A electronic search for literature was performed on the Google Scholar, PubMed, EMBASE, and Web of Science databases for related articles using multiple keywords. The keywords used were antimicrobial properties, mechanism of action, and current uses of Ag NPs in dentistry. The search was limited to articles published in the English language; no deadline was set for publications. Results. About 185 articles were considered relevant to the reported research out of 6420 electronic search results. Only the publications that were required were received after all abstracts were assessed for relevance. After a final electronic and manual search, roughly 47 research publications were found to be helpful in this study. Conclusion. The Ag NPs, unlike other biomaterials used in dentistry, have unique biological features and could be used in endodontics, restorative dentistry, periodontology, prosthetic dentistry, implantology, and oral malignancies, because of their antimicrobial, antifungal, and antiviral effects.
9 citations
Patent•
07 Jul 2020TL;DR: In this paper, a method for synthesizing metal nanoparticles can include combining a metallic nitrate with an extract of Kalanchoe blossfeldiana to form the metal particles, and adding an aqueous solution of sodium hydroxide (NaOH) to the zinc nitrate extract solution to form zinc oxide nanoparticles.
Abstract: A method for synthesizing metal nanoparticles can include combining a metallic nitrate with an extract of Kalanchoe blossfeldiana to form the metal nanoparticles. The method can include adding an aqueous solution of silver nitrate (AgNO3) to the extract of Kalanchoe blossfeldiana to form silver nanoparticles. The method can include dissolving zinc nitrate hexahydrate (Zn(NO3)2.6H2O) in an extract of Kalanchoe blossfeldiana to provide a zinc nitrate extract solution, stirring the zinc nitrate extract solution, and adding an aqueous solution of sodium hydroxide (NaOH) to the zinc nitrate extract solution to form zinc oxide nanoparticles.
1 citations