Growing and analyzing static biofilms
Reads0
Chats0
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
More filters
Journal ArticleDOI
Safety and efficacy of composite collagen–silver nanoparticle hydrogels as tissue engineering scaffolds
Emilio I. Alarcon,Klas I. Udekwu,Christopher W. Noel,Luke B.-P. Gagnon,Patrick K. Taylor,Branka Vulesevic,Madeline J. Simpson,Spyridon Gkotzis,Mohammed Mirazul Islam,Mohammed Mirazul Islam,Chyan-Jang Lee,Agneta Richter-Dahlfors,Thien-Fah Mah,Erik J. Suuronen,Juan C. Scaiano,Juan C. Scaiano,May Griffith,May Griffith +17 more
TL;DR: The safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering and an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant.
Journal ArticleDOI
Bdellovibrio bacteriovorus directly attacks Pseudomonas aeruginosa and Staphylococcus aureus Cystic fibrosis isolates.
Valerio Iebba,Valentina Totino,Floriana Santangelo,Antonella Gagliardi,Luana Ciotoli,A. Virga,Cecilia Ambrosi,Monica Pompili,Riccardo Valerio De Biase,Laura Selan,Marco Artini,Fabrizio Pantanella,Francesco Mura,Claudio Passariello,Mauro Nicoletti,Lucia Nencioni,Maria Trancassini,Serena Quattrucci,Serena Schippa +18 more
TL;DR: The dual foraging system against Gram-negative (periplasmic) and Gram-positive (epibiotic) prey could suggest the use of B. bacteriovorus as a “living antibiotic” in CF, even if further studies are required to simulate its in vivo predatory behavior.
Journal ArticleDOI
Pseudomonas aeruginosa tssC1 Links Type VI Secretion and Biofilm-Specific Antibiotic Resistance
TL;DR: It is demonstrated that Pseudomonas aeruginosa tssC1, a gene implicated in type VI secretion (T6S), is important for resistance of biofilms to a subset of antibiotics.
Journal ArticleDOI
OmpA-Mediated Biofilm Formation Is Essential for the Commensal Bacterium Sodalis glossinidius To Colonize the Tsetse Fly Gut
TL;DR: It is determined that Sodalis forms biofilms in the tsetse gut and that this process is influenced by the Sodalis outer membrane protein A (OmpA).
Journal ArticleDOI
Chlorhexidine activity against bacterial biofilms.
Pauline Cordenonsi Bonez,Camilla Filippi dos Santos Alves,Tanise Vendruscolo Dalmolin,Vanessa Albertina Agertt,Caren Rigon Mizdal,Vanessa da Costa Flores,Jaciane Baggiotto Marques,Roberto Christ Vianna Santos,Marli Matiko Anraku de Campos +8 more
TL;DR: Chlorhexidine demonstrated excellent antimicrobial activity for most microorganisms tested in their free form, but was less effective against biofilms of Acinetobacter baumannii, Escherichia coli, methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa.
References
More filters
Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.