Growing and analyzing static biofilms
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Cites methods from "Growing and analyzing static biofil..."
...For the CV assay (Merritt et al., 2005), themediumwas carefully removedwith the aid of amicropipette and the biofilms were washed three times by adding 225 μl of phosphate buffered saline (PBS, NaCl 8 g/L; KCl 0....
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...Biofilm formation was measured under static conditions as described elsewhere (Merritt et al., 2005)....
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...For the CV assay (Merritt et al., 2005), themediumwas carefully removedwith the aid of amicropipette and the biofilms were washed three times by adding 225 μl of phosphate buffered saline (PBS, NaCl 8 g/L; KCl 0.2 g/l; Na2HPO4 1.44 g/l; KH2PO4 0.24 g/l; pH 7.4 (Merck)) to remove unattached cells....
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...This technique is widely used for various bacterial species (Merritt et al., 2005)....
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65 citations
65 citations
References
1,980 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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918 citations
885 citations
"Growing and analyzing static biofil..." refers methods in this paper
...Colony biofilms Colony biofilms (see Basic Protocol 3) have typically been used for the purpose of determining antibiotic resistance (Anderl et al., 2000; Walters et al., 2003)....
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820 citations
343 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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