Growing and analyzing static biofilms
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38 citations
Cites methods from "Growing and analyzing static biofil..."
...Crystal violet staining of biofilm plates was performed using standard protocols (Merritt et al., 2011), as follows: Non-adherent cells were washed off by submerging the plate in water, and adherent cells were stained with 0....
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...Biofilm assays were performed as previously described (Merritt et al., 2011)....
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...Crystal violet staining of biofilm plates was performed using standard protocols (Merritt et al., 2011), as follows: Non-adherent cells were washed off by submerging the plate in water, and adherent cells were stained with 0.1% crystal violet, washed twice with water, dried, and resuspended in 33%…...
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...Biofilm Microplate Assay and Viability Assay Biofilm assays were performed as previously described (Merritt et al., 2011)....
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38 citations
Cites methods from "Growing and analyzing static biofil..."
...400 Da) [17] which were prepared as follows: they were cut in circles to match the Petri dish, washed first in boiling 5% Na2CO3 and then in boiling water for 15 min, autoclaved and placed over Petri dishes, containing YEM or MH medium....
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...After incubating at 30uC for 48 h, bacterial growth was evaluated by reading the turbidity at 590 nm, while biofilm biomass was estimated at 570 nm after crystal violet staining [17]....
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38 citations
38 citations
38 citations
References
1,980 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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918 citations
885 citations
"Growing and analyzing static biofil..." refers methods in this paper
...Colony biofilms Colony biofilms (see Basic Protocol 3) have typically been used for the purpose of determining antibiotic resistance (Anderl et al., 2000; Walters et al., 2003)....
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820 citations
343 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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