Growing and analyzing static biofilms
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TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Bidirectional alterations in antibiotics susceptibility in Staphylococcus aureus-Pseudomonas aeruginosa dual-species biofilm.
Elena Y. Trizna,Maria N Yarullina,Diana R. Baidamshina,Anna V. Mironova,Farida Akhatova,Elvira Rozhina,Rawil Fakhrullin,Alsu M Khabibrakhmanova,Almira Kurbangalieva,Mikhail I. Bogachev,Airat R. Kayumov +10 more
TL;DR: It is shown that S. aureus also modulates the susceptibility of P. aeruginosa to antibiotics in mixed cultures, which opens promising perspectives to increase the antimicrobial treatment efficacy of the wounds infected with nosocomial pathogens by the transplantation of the skin residential microflora.
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Production of Siderophores Increases Resistance to Fusaric Acid in Pseudomonas protegens Pf-5
TL;DR: It is demonstrated that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens and elucidate the mechanisms that enable the bacterium to survive in the presence of theMycotoxin.
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Biofilm formation of Bacillus cereus under food-processing-related conditions
TL;DR: Results indicate that food-industry-related conditions could promote B. cereus biofilm formation, which is relevant to food safety, and appears to be affected by surface properties, temperature, and carbon sources.
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Cadexomer iodine provides superior efficacy against bacterial wound biofilms in vitro and in vivo
Daniel James Fitzgerald,Paul J. Renick,Emma C. Forrest,Shannon P. Tetens,David N. Earnest,Jillian McMillan,Brett M. Kiedaisch,Lei Shi,Eric Roche +8 more
TL;DR: The superior efficacy for CI in these in vitro and in vivo models suggests CI topical products may represent a better choice to address established bacterial biofilm in chronic wounds.
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The effects of glutaraldehyde on the control of single and dual biofilms of Bacillus cereus and Pseudomonas fluorescens.
TL;DR: Dual speciesBiofilms demonstrated higher resistance and resilience to GLUT exposure than either of the single species biofilms, and biocide susceptibility of dual species planktonic cultures was an average of each single species.
References
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Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
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Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
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