Growing and analyzing static biofilms
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TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
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A comparative antimicrobial and toxicological study of gold(III) and silver(I) complexes with aromatic nitrogen-containing heterocycles: synergistic activity and improved selectivity index of Au(III)/Ag(I) complexes mixture
Nada D. Savić,Dusan Milivojevic,Biljana Đ. Glišić,Tatjana Ilic-Tomic,Jovana B. Veselinović,Aleksandar Pavic,Branka Vasiljevic,Jasmina Nikodinovic-Runic,Miloš I. Djuran +8 more
TL;DR: Synergistic antimicrobial effect and the selectivity index of 10 were achieved for the selected gold(III)/silver(I) complexes mixtures, as well as higher P. aeruginosa PAO1 biofilm disruption activity, and improved toxicity profile towards zebrafish embryos, in comparison to the single complexes.
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Transcriptional Activation of the mrkA Promoter of the Klebsiella pneumoniae Type 3 Fimbrial Operon by the c-di-GMP-Dependent MrkH Protein
Ji Yang,Jonathan J. Wilksch,Jason Tan,Dianna M Hocking,Chaille T. Webb,Trevor Lithgow,Roy M. Robins-Browne,Richard A. Strugnell +7 more
TL;DR: The results suggest that MrkH activates mrkA expression by interacting directly with RNA polymerase, to overcome the inefficient transcriptional initiation caused by the presence of defective core promoter elements.
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Antibiofilm activity of Actinobacillus pleuropneumoniae serotype 5 capsular polysaccharide.
Michael T. Karwacki,Daniel E. Kadouri,Meriem Bendaoud,Era A. Izano,Vandana Sampathkumar,Thomas J. Inzana,Jeffrey B. Kaplan +6 more
TL;DR: These findings suggest that the A. pleuropneumoniae serotype 5 capsule inhibits cell- to-cell and cell-to-surface interactions of other bacteria, and may lead to the development of new classes of antibiofilm agents for industrial and clinical applications.
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Melaleuca alternifolia nanoparticles against Candida species biofilms
Márcia Ebling de Souza,Leonardo Quintana Soares Lopes,Pauline Cordenonsi Bonez,André Gündel,Diego Stéfani T. Martinez,Michele Rorato Sagrillo,Janice Luehring Giongo,Rodrigo de Almeida Vaucher,Renata Platcheck Raffin,Aline Augusti Boligon,Roberto Christ Vianna Santos +10 more
TL;DR: The addition of TTO and its nanoparticles represented a significant reduction of biofilm formed by all Candida species, as well as a reduction of proteins and exopolysaccharides levels.
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The biofilm eradication activity of acetic acid in the management of periprosthetic joint infection.
TL;DR: The MBEC of acetic acid following a 10- or 20-minute treatment time exceeded its safety threshold, making these concentrations unsuitable as a topical debridement adjunct, however, a clinically acceptable concentration was still found to eliminate 96.1% of biofilm-associated MSSA following a 20- minutes treatment time.
References
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Genetic approaches to study of biofilms
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TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
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