Growing and analyzing static biofilms
Citations
25 citations
25 citations
Cites methods from "Growing and analyzing static biofil..."
...Previous work has demonstrated that the assay used here, a 96-well microtiter plate with crystal violet staining, has a high repeatability and broad applicability and is useful for the examination of the early stages of biofilm development (O’Toole et al., 1999; Merritt et al., 2005; Peeters et al., 2008; O’Toole, 2011)....
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...…that the assay used here, a 96-well microtiter plate with crystal violet staining, has a high repeatability and broad applicability and is useful for the examination of the early stages of biofilm development (O’Toole et al., 1999; Merritt et al., 2005; Peeters et al., 2008; O’Toole, 2011)....
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25 citations
Cites methods from "Growing and analyzing static biofil..."
...Crystal violet was used to stain the microtiter plates (10)....
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25 citations
25 citations
Cites methods from "Growing and analyzing static biofil..."
...The ability of strains to form biofilms on mannosylated bovine serum albumin (ManBSA; Sigma) was determined using a modification of the assay described by Merritt et al. (2005)....
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...Following overnight incubation with shaking at 37 uC the ability of bacteria to form a biofilm on ManBSA was determined using crystal violet as described elsewhere (Merritt et al., 2005)....
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References
1,980 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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918 citations
885 citations
"Growing and analyzing static biofil..." refers methods in this paper
...Colony biofilms Colony biofilms (see Basic Protocol 3) have typically been used for the purpose of determining antibiotic resistance (Anderl et al., 2000; Walters et al., 2003)....
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820 citations
343 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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