Growing and analyzing static biofilms
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TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Fast and Simple Tool for the Quantification of Biofilm-Embedded Cells Sub-Populations From Fluorescent Microscopic Images
Mikhail I. Bogachev,Oleg A. Markelov,Elena Y. Trizna,Diana R. Baydamshina,Pavel V. Zelenikhin,Regina Murtazina,Airat R. Kayumov +6 more
TL;DR: This algorithm is implemented as a simple software tool that includes an intuitive and user-friendly graphical interface for the initial adjustment of algorithm parameters to the microscopic imaging conditions as well as for the sequential analysis of homogeneous series of similar microscopic images without further user intervention.
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Bactericidal Compounds Controlling Growth of the Plant Pathogen Pseudomonas syringae pv. actinidiae, Which Forms Biofilms Composed of a Novel Exopolysaccharide
TL;DR: The formation, architecture, and composition of P. actinidiae biofilms are studied as a model to assess the efficacies of bactericidal compounds and Experimental evidence that P. syringae pv.
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Control of planktonic and sessile bacterial cells by essential oils
Maíra Maciel Mattos de Oliveira,Danilo Florisvaldo Brugnera,Josianne Arantes do Nascimento,Roberta Hilsdorf Piccoli +3 more
TL;DR: The antibacterial potential of essential oils (EOs) from Cinnamomum cassia bark and Melaleuca alternifolia and Cymbopogon flexuosus leaves was evaluated against planktonic and sessile cells of enteropathogenic Escherichia coli (EPEC) and Listeria monocytogenes.
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Measurement of Predation and Biofilm Formation under Different Ambient Oxygen Conditions Using a Simple Gasbag-Based System
Daniel E. Kadouri,Aimy Tran +1 more
TL;DR: Although the gasbag system was found to be suitable for culturing bacteria that require a low-oxygen environment, it was not capable of supporting, with its current configuration, the growth of obligate anaerobes in liquid or agar medium.
Journal ArticleDOI
Comparison of biofilm formation and antibiotic resistance pattern of Pseudomonas aeruginosa in human and environmental isolates.
TL;DR: Differences in genes involve in biofilm synthesis between human and environmental isolates are highly significant and the environmental isolate of P. aeruginosa stile are sensitive to most antibiotics because they lacks the antibiotic resistance genes.
References
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Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
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Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
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