Growing and analyzing static biofilms
Citations
15 citations
15 citations
15 citations
15 citations
Cites background or methods from "Growing and analyzing static biofil..."
...Impact of ZA and CA on E. coli growth and biofilm formation – ALI assay The impact of ZA and CA on growth of E. coli during the ALI assay was monitored by OD measurements during lag and exponential phase until the onset of biofilm development....
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...However, a careful re-investigation of the chemical transformations occurring during the assays at the ALI and on PTFE plates revealed that synthetic, pure ZA was not active....
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...UPLC/MS samples were prepared as described above just before the plates were submitted to biofilm evaluation (O’Toole et al. 1999; Merritt et al. 2005)....
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...UPLC/MS quantification showed increasing CA concentrations until ~40% of the initially present ZA was converted to CA. Biofilm formation by V. natriegens was evaluated using a PTFE plate assay since it was not possible to evaluate ALI assay data due to bacterial floc formation....
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...However, biofilm formation at the ALI was negatively affected by CA....
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15 citations
References
1,980 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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918 citations
885 citations
"Growing and analyzing static biofil..." refers methods in this paper
...Colony biofilms Colony biofilms (see Basic Protocol 3) have typically been used for the purpose of determining antibiotic resistance (Anderl et al., 2000; Walters et al., 2003)....
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820 citations
343 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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