Growing and analyzing static biofilms
Citations
117 citations
Cites methods from "Growing and analyzing static biofil..."
...Growth and analysis of static biofilm at aerobic conditions was performed as previously described (Merritt et al., 2005)....
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115 citations
Cites methods from "Growing and analyzing static biofil..."
...The biofilm formation was analyzed by different standard methods [9]....
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113 citations
Cites methods from "Growing and analyzing static biofil..."
...Attachment assays Assays to test the different alleles of the yfiBNR system were performed as described in [11,89]....
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112 citations
Cites background or methods from "Growing and analyzing static biofil..."
...The cultures were washed of planktonic cells and stained with crystal violet as previously described (42)....
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...In a crystal violet microtiter plate assay for biofilm formation (42), cultures of WT P....
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110 citations
Cites methods from "Growing and analyzing static biofil..."
...The protocols described in this unit include improvements described by other groups (Christensen et al., 1995; Cramton et al., 2001a; Deighton et al., 2001; Mack et al., 2001; Merritt et al., 2005) and those developed in the authors’ laboratory....
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References
1,980 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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918 citations
885 citations
"Growing and analyzing static biofil..." refers methods in this paper
...Colony biofilms Colony biofilms (see Basic Protocol 3) have typically been used for the purpose of determining antibiotic resistance (Anderl et al., 2000; Walters et al., 2003)....
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820 citations
343 citations
"Growing and analyzing static biofil..." refers methods in this paper
...While popularized in the mid-to-late 1990s (Mack et al., 1994; O’Toole et al., 1999), the assay in its typically used form is derived from a protocol published by Christensen et al. (1985)....
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