Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
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...Accordingly, a preferred alternative is to accurately quantify cell death by appropriate cytofluorometric or microscopy assays.(675) Moreover, long-term clonogenic assays should be employed when possible to measure the effective functional survival of cells....
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...As part of this analysis, it is necessary to examine the effect of the specific treatment, conditions or mutation on cell viability using several methods.(675) In the case of postmitotic cells such as neurons or retinal cells, cell death—and cell rescue by autophagy inhibition—can usually be established in vivo by morphological analysis,(676) and in culture by cell counts and/or measurement of the release of an enzyme such as LDH into the medium at early and late time points; however, a substantial amount of neuronal cell death occurs during neurogenesis, making it problematic to carry out a correct analysis in vivo or ex vivo....
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Cites background or methods from "Guidelines for the use and interpre..."
...Accordingly, a preferred alternative is to accurately quantify cell death by appropriate cytofluorometric or microscopy assays.(675) Moreover, long-term clonogenic assays should be employed when possible to measure the effective functional survival of cells....
[...]
...As part of this analysis, it is necessary to examine the effect of the specific treatment, conditions or mutation on cell viability using several methods.(675) In the case of postmitotic cells such as neurons or retinal cells, cell death—and cell rescue by autophagy inhibition—can usually be established in vivo by morphological analysis,(676) and in culture by cell counts and/or measurement of the release of an enzyme such as LDH into the medium at early and late time points; however, a substantial amount of neuronal cell death occurs during neurogenesis, making it problematic to carry out a correct analysis in vivo or ex vivo....
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2,238 citations
Cites methods from "Guidelines for the use and interpre..."
...However, these methods also have major drawbacks and must be interpreted with caution, especially when single parameters are being investigated.(13,15) Thus, it...
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