GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Citations
4,518 citations
Cites background from "GUS fusions: beta‐glucuronidase as ..."
...459-468), Dico,ostelium discoidllm (R. Firtel, pers. comm, quoted in Jefferson et al., 1986) or in almost any higher plant (Jefferson et al., 1987b), including tobacco, petunia, potato, tomato, Bras.6ca, maize, soybean, wheat, rice, barley, or Arahidopss The enzyme can tolerate large…...
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...The beta-glucuronidase gene has been chined and sequenced, and encodes a stable enzyme that has desirable properties fi)r the construction and analysis o fgene fusions(Jefferson, 1985;Jefferson et al., 1986a; Jefferson et al., 1987a; Jefferson c ta l ....
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...…the regulation of numerous genes in transgenic tobacco and potato, including CaMV35S promoter, ribulose bisphosphate, carboxylase small subunit, (Jefferson et al., 1987), chlorophyll a/b binding protein (Jefferson, Harkins, Bevan, Kavanagh and Bevan, submitted; Kavanagh, Jefferson and Bevan, in…...
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..., 1987b; Jefferson et al., 1987c)....
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...There is little or no detectable betaglucuronidase activity in Saccharomyces cerez,isiae, Dros,phi/~1 melalzogaster embryos, larvae and pupae (adults were not tested), certain strains of Caenorhahditis elegans (Jefferson et al., 1987a; Sebastiano et al., 1986, Ge~zetks....
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2,490 citations
Cites background from "GUS fusions: beta‐glucuronidase as ..."
...This capability is not available in the currently described plant Gateway vectors (Karimi et al., 2002), nor is the facility to induce gene expression....
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1,758 citations
Cites methods from "GUS fusions: beta‐glucuronidase as ..."
...Qualitative and quantitative measurements for GUS activity were performed according to Jefferson et al. (1987)....
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1,642 citations
1,589 citations
Cites methods from "GUS fusions: beta‐glucuronidase as ..."
...The most widely used binary vectors are BIN 19 [3] and its derivatives, the pBI series [24], and binary vectors pGA482 and pGA492 [1]....
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References
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