Hemizona assay and teratozoospermia: increasing sperm insemination concentrations to enhance zona pellucida binding *
TL;DR: In this paper, the authors evaluated the zona-binding capacity of patients with abnormal sperm morphology, using standard hemizona assay (HZA) conditions and increasing sperm insemination concentration during the assay and determined the minimum concentration of motile sperm from fertile controls necessary to validate HZA results, using sperm from teratozoospermic patients versus proven fertile controls.
About: This article is published in Fertility and Sterility.The article was published on 1990-09-01. It has received 54 citations till now. The article focuses on the topics: Abnormal sperm morphology & Sperm.
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TL;DR: Strict morphology is an excellent biomarker of sperm fertilizing capacity, independent of motility and concentration and may denote a poorer prognosis for establishing a pregnancy, even after a satisfactory fertilization rate is achieved.
173 citations
01 Apr 2010
TL;DR: A Practical Guide to Basic Laboratory Andrology - Libros de Medicina - Fertilidad y embarazo - 39,42
Abstract: This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.
99 citations
TL;DR: The fertilization and pregnancy rates correlate significantly with morphologically normal spermatozoa.
Abstract: In this study, a total of 95 ejaculates from infertile patients were investigated morphologically according to Kruger's strict criteria and 78 of the 95 ejaculates were stained for chromatin condensation with acidic aniline blue. Patients were divided into two groups based on the percentage of morphologically normal spermatozoa as follows: Men with normal sperm morphology 14% (Group 2). The relationship between percentage of normal sperm morphology and fertilization, cleavage and pregnancy rate was analysed. The rates were 33.7%, 57.1% and 0.0% respectively, in the first group. The corresponding values for the second group were 76.1%, 68.2% and 32.1%. The fertilization and pregnancy rates correlate significantly with morphologically normal spermatozoa. In regard to the percentage of morphologically normal spermatozoa stained with aniline blue, patients were divided into two groups: patients with 0-20% stained spermatozoa (Group I) and those with > 20% (Group 2). Fertilization and pregnancy rates were higher in the first group than in the second group (79.9%, 52.8% vs. 58.8%, 29.5%). The results demonstrate that chromatin condensation visualized by aniline blue staining is a good predictor for IVF outcome and should be considered besides morphology by sperm assessment for patients undergoing IVF treatment.
95 citations
TL;DR: The evaluation of sperm morphology by strict criteria is a simple, cost-effective method and can be used to guide the clinician and scientist on a day-to-day basis to make sound clinical decisions.
Abstract: This article attempts to evaluate the value of sperm morphology in assisted reproduction by summarizing a recent structured literature review covering the topic. New developments in the field of sperm morphology with emphasis on computer evaluation of morphology and its latest clinical application are highlighted, as well as the correlation between sperm functional tests and sperm morphology. Based on the correlation between the sperm functional tests and sperm morphology, as well as the latter's proven value as a predictor in in-vitro fertilization, one can assume that sperm morphology reflects function, although based on definition it is not a sperm functional test per se. The evaluation of sperm morphology by strict criteria is a simple, cost-effective method and can be used to guide the clinician and scientist on a day-to-day basis to make sound clinical decisions.
86 citations
TL;DR: Although the implantation and ongoing pregnancy rates are very low, ICSI seems to be the only treatment modality in cases where teratozoospermia was total with 100% abnormal head morphology.
Abstract: The present study was designed to determine the efficacy of intracytoplasmic sperm injection (ICSI) using spermatozoa with abnormal head morphology in 17 cases with total teratozoospermia. A total of 160 oocytes were retrieved and 144 metaphase II oocytes were injected. The fertilization and cleavage rates were 50.7 and 93.2% respectively. Fertilization failure occurred in two couples. A total of 54 embryos were transferred and pregnancy rates per initiated and per embryo transfer cycle were 17.6 and 20.0% respectively, while the clinical pregnancy rates per initiated and embryo transfer cycle were 11.8 and 13.3%. The implantation rate was 3.7% (2/54). Out of two pregnancies achieved, one resulted in abortion in the first trimester. The ongoing pregnancy rates per initiated and embryo transfer cycle were 5.88% (1/17) and 6.6% (1/15) respectively. Although the implantation and ongoing pregnancy rates are very low, ICSI seems to be the only treatment modality in cases where teratozoospermia was total with 100% abnormal head morphology.
84 citations
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TL;DR: In this article, the authors conducted a prospective study in women with bilateral tubal damage to determine whether there is a prognostic value in the percentage normal sperm morphologic features in a human in vitro fertilization (IVF) program.
1,153 citations
TL;DR: By evaluating sperm morphology with the proposed strict criteria, its predictive value in in vitro fertilization is enhanced.
1,095 citations
TL;DR: Sperm from fertile men exhibited significantly higher binding capacity to hemizonae compared with sperm from men who had fertilization failure during in vitro fertilization (IVF) treatment, demonstrating that the HZA may be a useful diagnostic tool in male infertility evaluations.
390 citations
TL;DR: It is concluded that patients with severe sperm head abnormalities have a lower ability to establish successful pregnancies, even though fertilization may be achieved.
192 citations
TL;DR: The demonstration of a compatibility between the gametes of man and gibbon, not shared with cercopithecids, constitutes further evidence for inclusion of the Hylobatidae within the Hominoidea.
Abstract: Human spermatozoa display unusually limited affinities in their interaction with oocytes of other species. They adhered to and, when capacitated, penetrated the vestments of the oocyte of an ape--the gibbon, Hylobates lar--both in vivo and in vitro. On the other hand, human spermatozoa would not even attach to the zona surface of sub-hominoid primate (baboon, rhesus monkey, squirrel monkey), nor to the non-primate eutherian oocytes tested. Among the apes the gibbon stands furthest from man. Thus, although the specificity of human spermatozoa is not confined to man alone, it probably is restricted to the Hominoidea. This study also suggests that the evolution of man and perhaps the other hominids has been accompanied by a restrictive change in the nature of the sperm surface which has limited and made more specific the complementary surface to which their spermatozoa may adhere. For the failure of human spermatozoa to attach to the zona surface of all non-hominoid oocytes stands in contrast to the behaviour of spermatozoa of the several other mammals studied which, in most combinations, adhered readily to foreign oocytes, including those of man. Taxonomically, the demonstration of a compatibility between the gametes of man and gibbon, not shared with cercopithecids, constitutes further evidence for inclusion of the Hylobatidae within the Hominoidea.
171 citations