Hemoglobin A2 values in sickle cell disease patients quantified by high performance liquid chromatography and the influence of alpha thalassemia.
Silvana Fahel da Fonseca,Tatiana Amorim,Antônio Purificação,Marilda Souza Goncalves,Ney Boa-Sorte +4 more
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Hb A2 levels are elevated in patients with Hb S or Hb C, and are directly influenced by the alpha thalassemia genotypes.Abstract:
a b s t r a c t Background: In sickle cell disease, the quantification of Hb A2 is important for the differentialread more
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Vertebrate and invertebrate respiratory proteins, lipoproteins and other body fluid proteins
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TL;DR: This book focuses on respiratory proteins, the broad hemoglobin family, as well as the molluscan and arachnid hemocyanins (and their multifunctional roles) and a number of other proteins, such as the hemerythrins; serum albumin; serum amyloid A; von Willebrand factor and its interaction with factor VIII; and C-reactive protein.
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Techniques for the Detection of Sickle Cell Disease: A Review.
TL;DR: An overview of the current and emerging techniques for sickle cell disease detection and highlights the different potential methods that could be applied to help the early diagnosis of SCD can be found in this paper.
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Hemoglobin A2 (HbA2) has a measure of unreliability in diagnosing β-thalassemia trait (β-TT).
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Knowledge of professional healthcare providers about sickle cell disease: Impact of a distance education course
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The importance of hemoglobin A2 determination.
TL;DR: Level was influenced by the genotype of -Thal, and the diagnosis of doubleheterozygous states, such as S- Thal, should be carried out care-fully, taking into consideration the limitations of the available laboratory techniques.
References
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Journal ArticleDOI
Interlaboratory comparison of current high-performance methods for HbA2.
TL;DR: A comparison study among the actual best performing techniques for HbA2 determination, comprising HPLC and CE methods, was performed.
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alpha-Thalassemia 2, 3.7 kb deletion and hemoglobin AC heterozygosity in pregnancy: a molecular and hematological analysis.
F. D. Couto,A. B. L. De Albuquerque,Elisângela Vitória Adorno,J. P. De Moura Neto,L. De Freitas Abbehusen,J. L. B. De Oliveira,M.G. dos Reis,M. De Souza Gonçalves,M. De Souza Gonçalves +8 more
TL;DR: The results emphasize the importance of diagnosis and follow-up of patients with hemoglobinopathy carriers during pregnancy in order to administer adequate therapy and avoid further complications for mothers and newborns.
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Effect of HbS in the determination of HbA2 with the Biorad Variant II analyzer
Christoforos Kalleas,Ioannis Tentes,Dimitrios Margaritis,Kostas Anagnostopoulos,Athina Toli,Dimitrios Pendilas,Georgios Bourikas,Constantinos Tsatalas,Alexandros H. Kortsaris +8 more
TL;DR: The Variant II analyzer gives reliable results for %HbA2 determination when no HbS is detectable in the samples, when compared to the alkaline electrophoresis gel.
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Effect of HbS in the determination of HbA2 with the Menarini HA-8160 analyzer and comparison with other instruments.
Kostas Anagnostopoulos,Ioannis Tentes,C. Kalleas,Dimitrios Margaritis,A. Toli,D. Pendilas,Georgios Bourikas,Costas Tsatalas,Alexandros H. Kortsaris +8 more
TL;DR: A strong positive bias in the %HbA2 values from the HA‐8160 is apparent in the presence of HbS in the samples, when compared with the alkaline electrophoresis, and the analytical imprecision and bias of the three HPLC instruments are comparable.
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Effect of HbS in the determination of HbA2 with the TOSOH HLC-723G7 analyzer and the HELENA Beta-Thal Quik column kit
Christoforos Kalleas,Ioannis Tentes,Dimitrios Margaritis,Kostas Anagnostopoulos,Athina Toli,Dimitrios Pendilas,Georgios Bourikas,Constantinos Tsatalas,Alexandros H. Kortsaris +8 more
TL;DR: Both the TOSOH HPLC and the manual column are reliable methods for %HbA(2) determination when no HbS is detectable in the samples, compared to the alkaline electrophoresis gel.