Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding
Joseph L. Kim,Morgenstern Kurt A,James P. Griffith,Maureen D. Dwyer,John A. Thomson,Murcko Mark A,Chao Lin,Paul R. Caron +7 more
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TLDR
The structure of the HCV NS3 RNA helicase domain complexed with a single-stranded DNA oligonucleotide has been solved to 2.2 A resolution and is a member of a superfamily of helicases, termed superfamily II.About:
This article is published in Structure.The article was published on 1998-01-15 and is currently open access. It has received 630 citations till now. The article focuses on the topics: RNA Helicase A & Helicase.read more
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Asymmetry in the assembly of the RNAi enzyme complex.
TL;DR: It is shown that the two strands of an siRNA duplex are not equally eligible for assembly into RISC, and it is suggested that single-stranded miRNAs are initially generated as siRNA-like duplexes whose structures predestine one strand to enter the RISC and the other strand to be destroyed.
Flaviviridae :T he Viruses and Their Replication
TL;DR: The present research attacked the Flavivirus infection through two mechanisms: Membrane Reorganization and the Compartmentalization and Assembly and Release of Particles from Flaviv virus-infected Cells and Host Resistance to Flaviviral Infection.
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Replication of hepatitis C virus
TL;DR: The development of complete cell-culture systems should now enable the systematic dissection of the entire viral lifecycle, providing insights into the hitherto difficult-to-study early and late steps.
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Structure and mechanism of helicases and nucleic acid translocases.
TL;DR: This review sets out to define a nomenclature for helicase and translocase enzymes based on current knowledge of sequence, structure, and mechanism, and delineate six superfamilies of enzymes, with examples of crystal structures where available.
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Replication of the hepatitis C virus
TL;DR: This review summarizes the current knowledge about HCV replication and describes attempts pursued in the last few years to establish efficient and reliable cell culture systems.
References
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The CCP4 suite: programs for protein crystallography
TL;DR: The CCP4 (Collaborative Computational Project, number 4) program suite is a collection of programs and associated data and subroutine libraries which can be used for macromolecular structure determination by X-ray crystallography.
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Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
TL;DR: Related sequences in both alpha and beta and in other enzymes that bind ATP or ADP in catalysis help to identify regions contributing to an adenine nucleotide binding fold in both ATP synthase subunits.
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Free R value: a novel statistical quantity for assessing the accuracy of crystal structures.
TL;DR: In this article, a statistical quantity (RfreeT) is defined to measure the agreement between observed and computed structure factor amplitudes for a 'test' set of reflections that is omitted in the modelling and refinement process.
Book
X-PLOR Version 3.1: A System for X-ray Crystallography and NMR
TL;DR: This manual to X-PLOR Version 3.1 presents the theoretical background, syntax and function of the programme and also provides a comprehensive list of references and sample input files with comments.
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The P-loop--a common motif in ATP- and GTP-binding proteins.
TL;DR: Current knowledge of P-loops is discussed with the additional aim of illustrating the fascinating relationship between protein sequence, structure and function.