Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.
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"Hepatitis E virus (HEV): molecular ..." refers methods in this paper
...The isolation of BETEXPCR2 completed the HEV genomic cDNA cloning....
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...The procedure yielded numerous clones by hybridization with primer D, of which BET-EXPCR2 is a representative example....
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...After several failed attempts at cloning the remaining 5’ end sequences by oligonucleotide hybridization of specifically primed cDNA libraries, an alternative expansion/enrichment procedure of PCR amplification of specifically primed G-tailed cDNA was applied (Tam et al., 1989)....
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...A and B represent synthetic oligonucleotides used for the generation and screening, respectively, of specifically primed cDNA libraries, The anchor PCR strategy using G-tailing and PCR (primer C) was used in the synthesis of primer extension libraries for the extreme 5’ end....
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...The modified cDNA was then amplified in a polymerase chain reaction (PCR) (Saiki et al., 1985; Mullis and Faloona, 1987) employing the same synthetic HEV primer and an oligo(dC) primer, both of which contained an EcoRl cloning site at the 5’ end....
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"Hepatitis E virus (HEV): molecular ..." refers methods in this paper
...The modified cDNA was then amplified in a polymerase chain reaction (PCR) (Saiki et al., 1985; Mullis and Faloona, 1987) employing the same synthetic HEV primer and an oligo(dC) primer, both of which contained an EcoRl cloning site at the 5’ end....
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"Hepatitis E virus (HEV): molecular ..." refers methods in this paper
...Total cellular RNA was isolated from normal and HEV(B)-infected cyno livers by the guanidinium-LiCI precipitation method (Cathala et al., 1983) and pofy(A)+ RNA was selected by one round of oligo(dT) cellulose chromatography (Aviv and Leder, 1972). cDNA library construction and screening....
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