High-Performance Liquid Chromatography
01 Jan 2009-
TL;DR: In this paper, a high-performance liquid chromatographic (HPLC) method is developed for the determination of the pharmacologically active terpenoids Ginkgolide A, B, and C and bilobalide in Ginkgo biloba L. leaves extract (GBE).
Abstract: A high-performance liquid chromatographic (HPLC) method is developed for the determination of the pharmacologically active terpenoids Ginkgolide A, B, and C and bilobalide in Ginkgo biloba L. leaves extract (GBE). The extracts are dissolved in methanol-water (1:4) and extracted by ethyl acetate after evaporation of the organic solvent, the residue is dissolved in methanol, and the terpenoids in the resultant solution are determined by means of HPLC on a C 1 8 column with methanol-water (23:77) as an eluent. The recovery provided by the method is above 96.5%, and the minimum concentration that can be determined is 80 μg of terpenoid per gram of GBE. The method is suitable for GBE production process control.
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TL;DR: In this article, a reversed-phase high-performance liquid chromatographic method has been developed for the separation and determination of the flavonoids found in the leaves and therapeutically used extracts of Ginkgo biloba.
171 citations
TL;DR: The main terpenes of Ginkgo biloba L. extracts have been separated by isocratic elution on a 3 μm C18 Spherical column using 2-propanol:water (10∶90) as eluent.
Abstract: The main terpenes ofGinkgo biloba L. extracts (bilobalide, ginkgolide A, ginkgolide B and ginkgolide C) have been separated by isocratic elution on a 3 μm C18 Spherical column using 2-propanol:water (10∶90) as eluent.
28 citations
22 citations