Highly improved adsorption selectivity of L-phenylalanine imprinted polymeric submicron/nanoscale beads prepared by modified suspension polymerization
TL;DR: In this article, the effects of pH, template and concentration of racemate solution on the performance of the phenylalanine (Phe) imprinted polymeric submicron/nanoscale beads were studied.
Abstract: Molecularly imprinted polymer (MIP) submicron/nanoscale beads selective for L-Phenylalanine (L-Phe) and D-Phe as well as non-imprinted beads were prepared by modified suspension polymerization involving agitation of the reaction mixture at high rotation speed under safe radical conditions. The effects of pH, template and concentration of racemate solution on the performance of the phenylalanine (Phe) imprinted polymeric submicron/nanoscale beads were studied. L-Phe-imprinted submicron/nanoscale beads prepared for the first time by modified suspension polymer- ization showed enhanced adsorption capacity and selectivity over those of D-Phe imprinted and non-imprinted beads. Maximum adsorption capacity, 0.35 mg/g, and selectivity, 1.62, of L-Phe imprinted submicron/nanoscale beads were higher than the adsorption capacities, 0.30 and 0.19 mg/g, and selectivities, 1.59 and 1.02, of D-Phe imprinted and non- imprinted submicron/nanoscale beads, respectively. FE-SEM analyses revealed that L- and D-Phe imprinted beads were larger (100 nm-1.5 µm) than non-imprinted nanobeads (100-800 nm). 13 C CP-MAS NMR spectroscopy helped in cor- relating the bead sizes and the extent of reaction during polymerization. Similarly, FT-IR study was used for evaluation of structural characteristics of the prepared Phe-imprinted and non-imprinted beads. The preparation of Phe-imprinted submicron/nanoscale beads with improved adsorption and separation properties and the study of effect of template on the size and performance of the prepared beads are suitable from both economical and research point of view in MIP field.
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TL;DR: A survey of the literature covering the development of molecular imprinting science and technology over the years 2004–2011 and efforts to apply these polymeric materials to a range of application areas is presented.
Abstract: Herein, we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004-2011. In total, 3779 references to the original papers, rev ...
413 citations
TL;DR: In this review, progresses in applications of enantio-selective recognition by MIPs will be critically reviewed for the recent period since 2007.
82 citations
TL;DR: A novel dummy template molecularly imprinted polymer (DMIP) based on a vinyl-SiO2 microspheres surface for the simultaneous selective recognition and enrichment of 18 amino acids was prepared via a surface molecular imprinting technique using theanine as a dummy template, indicating that the obtained DMIP sorbents have high selectivity.
41 citations
19 Oct 2020
TL;DR: In this article, a critical review of morphology design and control of spherical molecular imprinted polymers (MIPs) is presented, focusing on solid sphere, core-shell, hollow and mesoporous MIPs.
Abstract: Molecularly imprinted polymers (MIPs) display specific recognition ability for their template in shape, size and functional monomers. The morphology has an important influence on the binding capacity of MIPs, thus affecting the enrichment efficiency and detection sensitivity. In this critical review, we highlight the morphology design and control of spherical MIPs, mainly focusing on solid sphere, core–shell, hollow and mesoporous MIPs. The methods for preparation of MIPs of different morphologies are summarized, and typical TEM or SEM images are displayed. The influence of morphology on the application of MIPs is highlighted. Finally, we discuss the remaining challenges, and some significant attempts in further improving the morphology of MIPs are also proposed.
17 citations
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TL;DR: It was found that the template to functional monomer ratio had a pronounced influence on the MIP microparticle partial filling CEC performance as well as the size of the obtained microparticles.
Abstract: The use of molecularly imprinted polymer (MIP) microparticles in a partial filling application of capillary electrochromatography (CEC) has previously been shown successful for the enantiomer separation of propranolol. In this investigation, the influence of some important parameters in the preparation protocol, i.e., template to monomer ratio, type of cross-linker and functional monomers, and the effect of separation condition, i.e., organic modifier content, pH and the temperature of the column, on the electrochromatographic behavior of the MIP microparticles were studied. It was found that ethyleneglycol dimethacrylate (EDMA), having two reactive double bonds, was superior in terms CEC performance to trimethylpropane trimethacrylate (TRIM) and pentaerythritol tetraacrylate (PETEA) having three and four double bonds, respectively. The use of weak functional monomers, i.e., monomers lacking a strong interaction with the template, was shown to increase the separation efficiency. It was found that the template to functional monomer ratio had a pronounced influence on the MIP microparticle partial filling CEC performance as well as the size of the obtained microparticles. The use of a partial filling technique realizes the use of a new MIP phase in every new separation as well as the ability of altering the selectivity of the separation column and length of the MIP without the need for column switching.
76 citations
TL;DR: In this article, the imprinted polymer beads for solid-phase extraction (SPE) purposes were prepared by suspension polymerization, using trimethylolpropane trimethacrylate as cross-linker, and 2-vinylpyridine, 2-acrylamido-2-methylpropanesulfonic acid as bifunctional monomers in an aqueous system.
Abstract: Tyrosine imprinted polymer beads for solid-phase extraction (SPE) purposes were prepared by suspension polymerization, using trimethylolpropane trimethacrylate as cross-linker, and 2-vinylpyridine and 2-acrylamido-2-methylpropanesulfonic acid as bifunctional monomers in an aqueous system. The SPE evaluation showed that, except for the good regenerating properties, the imprinted polymers prepared by suspension polymerization exhibited a considerable binding specificity to tyrosine, and had similar adsorbing and molecule separating properties as those prepared by seed swelling and suspension polymerization, although the size distribution was slightly wider. The suspension polymerization technique is less time-consuming, simpler and easier to carry out.
70 citations
TL;DR: These results agree with a binding site model involving more than one carboxylic acid group, providing charge complementarity and hydrogen bond donors for binding of L-PA, the template.
69 citations
TL;DR: The mechanism underlying the release of S-propranolol from the MIP-NOM composite membrane was found to involve specific adsorption and mobility of this enantiomer at the binding site in the MIA as the latter undergo a transition from the dry to wet state.
64 citations
TL;DR: In this paper, the authors present a review of recent advances in the molecular imprinting area, with special emphasis on the use of controlled polymerization methods, their benefits, and current limitations.
Abstract: Molecularly imprinted polymers (MIPs) are tailor-made biomimetic receptors that are obtained by polymerization in the presence of molecular templates. They contain binding sites for target molecules with affinities and specificities on a par with those of natural receptors such as antibodies, hormone receptors, or enzymes. A great majority of the literature in the field describes materials based on polymers obtained by free radical polymerization. In order to solve general problems associated with MIPs, in particular their heterogeneity in terms of inner morphology and distribution of binding site affinities, it has been suggested to use modern methods of controlled/living radical polymerization for their synthesis. This also facilitates their generation in the form of nanomaterials, nanocomposites, and thin films, a strong recent trend in the field. The present paper reviews recent advances in the molecular imprinting area, with special emphasis on the use of controlled polymerization methods, their benefits, and current limitations.
56 citations