Journal ArticleDOI
How to study proteins by circular dichroism
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TLDR
The basis of the CD approach and its application to the study of proteins, and clear guidelines on how reliable data can be obtained and analysed are presented.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2005-08-10. It has received 3023 citations till now. The article focuses on the topics: Circular dichroism.read more
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Journal ArticleDOI
Using circular dichroism spectra to estimate protein secondary structure
TL;DR: This protocol details the basic steps of obtaining and interpreting CD data, and methods for analyzing spectra to estimate the secondary structural composition of proteins.
Journal ArticleDOI
Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases
Lee Whitmore,Bonnie A. Wallace +1 more
TL;DR: Recent developments in the analysis of protein secondary structures, including features of the DICHROWEB analysis webserver are discussed, which greatly improve and facilitate the analyses of CD spectra.
Journal ArticleDOI
Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions
TL;DR: Circular dichroism is an excellent spectroscopic technique for following the unfolding and folding of proteins as a function of temperature and may be used to determine the van't Hoff enthalpy and entropy of unfolding, the midpoint of the unfolding transition and the free energy of unfolding.
Journal ArticleDOI
Prediction of protein secondary structure from circular dichroism using theoretically derived spectra.
TL;DR: This study used DichroCalc to calculate the theoretical CD spectra of a nonredundant set of structures representing most proteins in the PDB, and applied a straightforward approach for predicting protein secondary structure content using these theoreticalCD spectra as reference set.
Journal ArticleDOI
Chiral Inorganic Nanostructures
TL;DR: This review will cover both experiment and theory of chiral nanostructures starting with the origin and multiple components of mirror asymmetry of individual NPs and their assemblies, and shall consider four different types of chirality in nanostructure and related physical, chemical, and biological effects.
References
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Journal ArticleDOI
Calculation of protein extinction coefficients from amino acid sequence data
Stanley C. Gill,P H von Hippel +1 more
TL;DR: In this article, a method for calculating accurate molar extinction coefficients for proteins at 280 nm, simply from knowledge of the amino acid composition, was presented, and the method was calibrated against 18 "normal" globular proteins.
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How to measure and predict the molar absorption coefficient of a protein.
TL;DR: The studies reported here suggest that the Edelhoch method is the best method for measuring ε for a protein, which can best be predicted with this equation.
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Hydrogen ion buffers for biological research.
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DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data.
Lee Whitmore,Bonnie A. Wallace +1 more
TL;DR: The DICHROWEB web server enables on-line analyses of circular dichroism (CD) spectroscopic data, providing calculated secondary structure content and graphical analyses comparing calculated structures and experimental data.
Journal ArticleDOI
Structure of 20S proteasome from yeast at 2.4 A resolution.
Michael Groll,Lars Ditzel,Jan Löwe,Daniela Stock,Matthias Bochtler,Hans D. Bartunik,Robert Huber +6 more
TL;DR: Two β-type subunits are processed to an intermediate form, indicating that an additional nonspecific endopeptidase activity may exist which is important for peptide hydrolysis and for the generation of ligands for class I molecules of the major histocompatibility complex.