scispace - formally typeset
Search or ask a question
Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

23 Jun 1998-Bioscience, Biotechnology, and Biochemistry (Japan Society for Bioscience, Biotechnology, and Agrochemistry)-Vol. 62, Iss: 6, pp 1201-1204
TL;DR: An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract: An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.
Citations
More filters
Journal ArticleDOI
TL;DR: Three methods widely employed in the evaluation of antioxidant activity, namely 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and beta-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts.
Abstract: Three methods widely employed in the evaluation of antioxidant activity, namely 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and β-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS-GC methods but not for the DPPH method. The complex composition of the extracts and partition phenomena affected their activity in each assay. The value of the BCBT method appears to be limited to less polar samples. Although slow, the HS-GC method is preferable for assessing the antioxidant inhibitory properties on the formation of unwanted secondary volatile products. Being rapid, simple and independent of sample polarity, the DPPH method is very convenient for the quick screening of many samples for radical scavenging activity. Copyright © 2001 John Wiley & Sons, Ltd.

1,640 citations

Journal ArticleDOI
TL;DR: A critical review on various developments to the DPPH method is presented, which has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them.
Abstract: α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

1,290 citations


Cites background or methods from "HPLC method for evaluation of the f..."

  • ...It is also observed that activity of natural antioxidants often decreases during their isolation and purification due to decomposition (Yamaguchi et al. 1998)....

    [...]

  • ...In HPLC-FIA method, the separated analytes react postcolumn with DPPH• (Koleva et al. 2000) and the induced scavenging is detected as a negative peak....

    [...]

  • ...2004) and HPLC-FIA (Yamaguchi et al. 1998) have been implemented in recent years....

    [...]

  • ...%Antioxidant activity ¼ E radicalð Þ E standardð Þ=E radicalð Þ ; where E is the extinction coefficient of DPPH. a. Inclusion of automation Several automation in the original DPPH assay, based on flow injection analysis (FIA) (Ukeda et al. 2002; Ukeda 2004), sequential injection analysis (SIA) (Polasek et al. 2004) and HPLC-FIA (Yamaguchi et al. 1998) have been implemented in recent years....

    [...]

  • ...…of DPPH. a. Inclusion of automation Several automation in the original DPPH assay, based on flow injection analysis (FIA) (Ukeda et al. 2002; Ukeda 2004), sequential injection analysis (SIA) (Polasek et al. 2004) and HPLC-FIA (Yamaguchi et al. 1998) have been implemented in recent years....

    [...]

Journal ArticleDOI
TL;DR: In this article, a b-carotene-linoleic acid (linoleate) model system was used to evaluate the scavenging effect on the DPPH free radical and capacity to scavenge hydroxyl free radicals.

1,067 citations

Journal ArticleDOI
TL;DR: The antioxidant properties of Sechium edule extracts were tested by three established in vitro methods, namely reducing power, β-carotene linoleate model and 1,1- diphenyl-2 picrylhydrazyl (DPPH) radical-scavenging.

928 citations

Journal ArticleDOI
TL;DR: It was observed that all samples of ethanolic extracts of propolis showed free radical-scavenging activity in terms of scavenging of the radical DPPH but the highest activities were found for samples from Tucumán and Santiago del Estero, which may justify their use as a source of natural antioxidants.

889 citations

References
More filters
Journal ArticleDOI
01 Apr 1958-Nature
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Abstract: METHODS for measuring antioxidants and appraising antioxidant activity appear to be of two general types. If the chemical nature of the antioxidant is known, one may strive for a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups. Alternatively one may observe the inhibition of some natural oxidative process such as the β-oxidation of fats, as a function of the added antioxidant.

10,611 citations

Journal ArticleDOI
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.

4,440 citations

Journal ArticleDOI
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.

3,489 citations

Journal ArticleDOI
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.

3,331 citations

Journal ArticleDOI
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
Abstract: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea, and black tea) was investigated. All tea extracts exhibited markedly antioxidant activity and reducing power, especially oolong tea, which inhibited 73.6% peroxidation of linoleic acid. Tea extracts exhibited a 65-75% scavenging effect on superoxide at a dose of 1 mg and 30 - 60% scavenging effect on hydrogen peroxide at a dose of 400 microgram. They scavenged 100% hydroxyl radical at a dosage of 4 mg except the black tea. Tea extracts also showed 50 - 70% scavenging effect on alpha, alpha-diphenyl-beta-picrylhydrazyl radical. The antioxidant activity and the scavenging effects on active oxygen decreased in the order semifermented tea > nonfermented tea > fermented tea. Tea extracts showed strong antimutagenic action against five indirect mutagens, i.e., AFB1, Trp-P-1, Glu-P-1, B[a]P, and IQ, especially oolong and pouchong teas. The antioxidant effect of tea extracts was well correlated to their antimutagenicity in some cases but varied with the mutagen and antioxidative properties.

2,436 citations