HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
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...It is also observed that activity of natural antioxidants often decreases during their isolation and purification due to decomposition (Yamaguchi et al. 1998)....
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...In HPLC-FIA method, the separated analytes react postcolumn with DPPH• (Koleva et al. 2000) and the induced scavenging is detected as a negative peak....
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...2004) and HPLC-FIA (Yamaguchi et al. 1998) have been implemented in recent years....
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...%Antioxidant activity ¼ E radicalð Þ E standardð Þ=E radicalð Þ ; where E is the extinction coefficient of DPPH. a. Inclusion of automation Several automation in the original DPPH assay, based on flow injection analysis (FIA) (Ukeda et al. 2002; Ukeda 2004), sequential injection analysis (SIA) (Polasek et al. 2004) and HPLC-FIA (Yamaguchi et al. 1998) have been implemented in recent years....
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...…of DPPH. a. Inclusion of automation Several automation in the original DPPH assay, based on flow injection analysis (FIA) (Ukeda et al. 2002; Ukeda 2004), sequential injection analysis (SIA) (Polasek et al. 2004) and HPLC-FIA (Yamaguchi et al. 1998) have been implemented in recent years....
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