Journal ArticleDOI
Hydrazone ligation assisted DNAzyme walking nanomachine coupled with CRISPR-Cas12a for lipopolysaccharide analysis
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TLDR
In this article, hydrazone ligation assisted DNAzyme walking nanomachine is explored to couple with CRISPR-Cas12a trans-cleavage to enhance the sensitivity for target detection.About:
This article is published in Analytica Chimica Acta.The article was published on 2021-08-22. It has received 10 citations till now.read more
Citations
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Journal ArticleDOI
Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review.
TL;DR: A comprehensive review of CRISPR/Cas-based tools for non-nucleic acid target detection can be found in this article , where the authors summarize the strategies and prospects of these tools in this field.
Journal ArticleDOI
Label-Free Resonance Rayleigh Scattering Amplification for Lipopolysaccharide Detection and Logical Circuit by CRISPR/Cas12a-Driven Guanine Nanowire Assisted Non-Cross-Linking Hybridization Chain Reaction.
TL;DR: In this paper , a CRISPR-derived resonance Rayleigh scattering (RRS) amplification strategy and logical circuit based on a guanine nanowire (G-wire) assisted non-cross-linking hybridization chain reaction (GWancHCR) for label-free detection of lipopolysaccharide (LPS).
Journal ArticleDOI
CRISPR/Cas12a system responsive DNA hydrogel for label-free detection of non-glucose targets with a portable personal glucose meter.
TL;DR: In this paper , a Personal Glucose Meter (PGM) was used for sensitive detection of non-glucose targets: N-gene and PCB77, respectively.
Journal ArticleDOI
Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets
TL;DR: In this paper , the authors review current advances in aptamer-based CRISPR-Cas sensors for point-of-care setting diagnostics and provide novel insights into developing CRISpl-Cas-based sensors using ssDNA aptamers with high efficiency and specificity.
Journal ArticleDOI
Magnetism-Controllable Catalytic Activity of DNAzyme.
Wenxing Li,Hao Wang,Shiqi Yang,Albertina N Isak,Yuchen Song,Fan Zhang,Dongsheng Mao,Xiaoli Zhu +7 more
TL;DR: This system provides a reusable platform for the control of enzyme catalytic activity through magnetism, which provides guidance for further application in some related scientific research, especially the regulation of the activity of conformation-dependent polymers (DNAzymes, aptamers, and peptides).
References
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Journal ArticleDOI
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Janice S. Chen,Enbo Ma,Lucas B. Harrington,Maria Da Costa,Xinran Tian,Joel M. Palefsky,Jennifer A. Doudna +6 more
TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.
Journal ArticleDOI
Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence
Alyssa B. Chinen,Chenxia M. Guan,Jennifer R. Ferrer,Stacey N. Barnaby,Timothy J. Merkel,Chad A. Mirkin +5 more
TL;DR: Overcoming Limitations in Nanoparticle Drug Delivery: Triggered, Intravascular Release to Improve Drug Penetration into Tumors and Design Considerations for Tumour-Targeted Nanoparticles.
Journal ArticleDOI
Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor.
TL;DR: The first CRISPR Cas12a (cpf1) based electrochemical biosensor (E-CRISPR) is reported, which is more cost-effective and portable comparing with optical transduction based biosensing systems and could be a powerful enabler for wide developments of portable, accurate, and cost-efficient point-of-care diagnostic systems.
Journal ArticleDOI
Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing
TL;DR: A DNA nanomachine, built from a DNA-functionalized gold nanoparticle (DNA-AuNP), which moves a DNA walker along a three-dimensional (3-D) DNA- AuNP track and executes the task of releasing payloads, which allows it to be tailored into a DNA nanosensor that is able to achieve rapid, isothermal, and homogeneous signal amplification for specific nucleic acids in both buffer and a complicated biomatrix.
Journal ArticleDOI
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules.
Mindong Liang,Mindong Liang,Zilong Li,Weishan Wang,Jiakun Liu,Jiakun Liu,Leshi Liu,Guoliang Zhu,Loganathan Karthik,Man Wang,Ke-Feng Wang,Zhong Wang,Jing Yu,Yuting Shuai,Jiaming Yu,Lu Zhang,Zhiheng Yang,Chuan Li,Qian Zhang,Tong Shi,Liming Zhou,Feng Xie,Huanqin Dai,Xueting Liu,Jie Zhang,Guang Liu,Ying Zhuo,Buchang Zhang,Chenli Liu,Shanshan Li,Xuekui Xia,Yaojun Tong,Yanwen Liu,Gil Alterovitz,Gao-Yi Tan,Lixin Zhang +35 more
TL;DR: CaT-SMelor is presented which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range and directly measured the uric acid concentration in clinical human blood samples, indicating a great potential of CaT- SMelor in the detection of small molecules.