Journal ArticleDOI
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Yoshiyuki Nakano,Kozi Asada +1 more
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Observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ASCorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione and a preliminary characterization of the chloroplast peroxidase is given.Abstract:
Intact spinach chloroplasts scavenge hydrogen peroxide with a peroxidase that uses a photoreductant as the electron donor, but the activity of ruptured chloroplasts is very low [Nakano and Asada (1980) Plant & Cell Physiol. 21: 1295]. Ruptured spinach chloroplasts recovered their ability to photoreduce hydrogen peroxide with the concomitant evolution of oxygen after the addition of glutathione and dehydroascorbate (DHA). In ruptured chloroplasts, DHA was photoreduced to ascorbate and oxygen was evolved in the process in the presence of glutathione. DHA reductase (EC 1.8.5.1) and a peroxidase whose electron donor is specific to L-ascorbate are localized in chloroplast stroma. These observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ascorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione. A preliminary characterization of the chloroplast peroxidase is given.read more
Citations
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THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons
TL;DR: Whenever the water-water cycle operates properly for scavenging of active oxygens in chloroplasts, it also effectively dissipates excess excitation energy under environmental stress.
Journal ArticleDOI
Magnesium Deficiency and High Light Intensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase, and Glutathione Reductase in Bean Leaves
Ismail Cakmak,Horst Marschner +1 more
TL;DR: The results demonstrate the role of both light intensity and Mg nutritional status on the regulation of O(2) (.-) and H( 2)O(3) scavenging enzymes in chloroplasts.
Journal ArticleDOI
Ascorbate peroxidase – a hydrogen peroxide-scavenging enzyme in plants
TL;DR: Amino acid sequence and other molecular properties indicate that ascorbate peroxidase resembles cytochrome c peroxIDase from fungi rather than guaiacol per oxidase from plants, and it is proposed that the plant and yeast hydrogen peroxide-scavenging peroxidsases have the same ancestor.
Journal ArticleDOI
Regulation and function of ascorbate peroxidase isoenzymes
Shigeru Shigeoka,Takahiro Ishikawa,Masahiro Tamoi,Yoshiko Miyagawa,Toru Takeda,Yukinori Yabuta,Kazuya Yoshimura +6 more
TL;DR: It is clear that a high level of endogenous ascorbate is essential effectively to maintain the antioxidant system that protects plants from oxidative damage due to biotic and abiotic stresses.
Journal ArticleDOI
Nitric Oxide Ameliorates Zinc Oxide Nanoparticles Phytotoxicity in Wheat Seedlings: Implication of the Ascorbate-Glutathione Cycle.
Durgesh Kumar Tripathi,Rohit Kumar Mishra,Swati Singh,Samiksha Singh,Kanchan Vishwakarma,Shivesh Sharma,Vijay Pratap Singh,Prashant Kumar Singh,Sheo Mohan Prasad,Nawal Kishore Dubey,Avinash C. Pandey,Shivendra V. Sahi,Devendra Kumar Chauhan +12 more
TL;DR: The results of the present study have shown the role of NO in the reducing of ZnONPs toxicity through the regulation of accumulation ofZn as well as the functioning of the AsA–GSH cycle.
References
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Journal ArticleDOI
The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism.
TL;DR: It is proposed that glutathione functions to stabilise enzymes of the Calvin cycle, and it may also act to keep ascorbic acid in chloroplasts in the reduced form.
Journal ArticleDOI
Starch gel electrophoresis of enzymes—A compilation of recipes
Charles R. Shaw,Rupi Prasad +1 more
TL;DR: Most of the methods outlined here have been used primarily for tissue extracts from mammalian species, and a few have been developed only on plants or lower animals, but this is not to say that they will not work on higher organisms.
Journal ArticleDOI
Generation of superoxide anion by the NADH dehydrogenase of bovine heart mitochondria.
J F Turrens,A Boveris +1 more
TL;DR: An O2- -dependent autocatalytic process that requires NADH, submitochondrial particles and adrenaline is described.
Journal ArticleDOI
Role of ubiquinone in the mitochondrial generation of hydrogen peroxide
TL;DR: Parallel measurements of H2O2 production, succinate dehydrogenase and succinate-cytochrome c reductase activities show that peroxide generation by ubiquin one-supplemented membranes is a monotonous function of the reducible ubiquinone content, whereas the other two measured activities reach saturation at relatively low concentrations of reducible quinone.
Journal ArticleDOI
Chloroplast Glutathione Reductase
TL;DR: Glutathione reductase activity is present in spinach chloroplasts and a possible role in chloroplastics is proposed and the pH dependence and substrate concentration for half-maximal rate are reported.
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