Journal ArticleDOI
Hydrophilic interaction chromatography.
Petrus Hemström,Knut Irgum +1 more
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TLDR
The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.Abstract:
Separation of polar compounds on polar stationary phases with partly aqueous eluents is by no means a new separation mode in LC. The first HPLC applications were published more than 30 years ago, and were for a long time mostly confined to carbohydrate analysis. In the early 1990s new phases started to emerge, and the practice was given a name, hydrophilic interaction chromatography (HILIC). Although the use of this separation mode has been relatively limited, we have seen a sudden increase in popularity over the last few years, promoted by the need to analyze polar compounds in increasingly complex mixtures. Another reason for the increase in popularity is the widespread use of MS coupled to LC. The partly aqueous eluents high in ACN with a limited need of adding salt is almost ideal for ESI. The applications now encompass most categories of polar compounds, charged as well as uncharged, although HILIC is particularly well suited for solutes lacking charge where coulombic interactions cannot be used to mediate retention. The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.read more
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Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more
TL;DR: Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases and their applications for separations of polar compounds in complex matrices.
Journal ArticleDOI
Stationary and mobile phases in hydrophilic interaction chromatography: a review
TL;DR: This review deals with recent advances in the development of HILIC phase separation systems with special attention to the properties of stationary phases.
Journal ArticleDOI
A review of current trends and advances in modern bio-analytical methods: chromatography and sample preparation.
Lucie Nováková,Hana Vlčková +1 more
TL;DR: UHPLC has recently become a wide-spread analytical technique in many laboratories which focus on fast and sensitive bio-analytical assays and the key advantages are the increased speed of analysis, higher separation efficiency and resolution, higher sensitivity and much lower solvent consumption as compared to other analytical approaches.
Journal ArticleDOI
Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides.
TL;DR: Electrostatic repulsion-hydrophilic interaction chromatography affords convenient separations of highly charged peptides that cannot readily be resolved by other means, including peptides, amino acids, and nucleotides.
Journal ArticleDOI
Analytical strategies for LC–MS-based targeted metabolomics ☆
TL;DR: It is suggested that MRM preferred when targeting a more limited number of analytes, and full scan preferred for its potential ability to bridge targeted and untargeted metabolomics, are viable solutions.
References
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Journal ArticleDOI
Thermodynamics of High Polymer Solutions
TL;DR: In this paper, a statistical treatment of high polymer solutions has been carried out on the basis of an idealized model, originally proposed by Meyer, which is analogous to the one ordinarily assumed in the derivation of the ''ideal'' solution laws for molecules of equal size.
Journal ArticleDOI
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
TL;DR: Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.
Journal ArticleDOI
Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: the yeast proteome.
TL;DR: The combination of strong cation exchange (SCX) and reversed-phase (RP) chromatography to achieve two-dimensional separation prior to MS/MS and 1,504 yeast proteins were unambiguously identified in this single analysis.
Journal ArticleDOI
Cucurbituril homologues and derivatives: new opportunities in supramolecular chemistry.
TL;DR: This Account is a compilation of recent literature covering the syntheses of the homologues and derivatives, and their supramolecular chemistry of cucurbituril, a synthetic receptor.
Journal ArticleDOI
Rapid Commun. Mass Spectrom.10. 1629-1637 (1996) Matrix-assisted Laser Desorption/Ionisation Mass Spectrometry in Milk Science
TL;DR: In this article, the interpretation of the mass spectra of new phosphorus containing organic compounds is presented, where the phosphorylated hydrazono group is condensed on the annular carbon from the 3-methyl-2-3Hbenzoxazolone ring.
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Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
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