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Journal ArticleDOI: 10.1016/J.BIORTECH.2021.124920

Hydroxylamine addition enhances fast recovery of anammox activity suffering Cr(VI) inhibition.

02 Mar 2021-Bioresource Technology (Elsevier)-Vol. 329, pp 124920-124920
Abstract: Hydroxylamine (NH2OH), one of the most important intermediates of anammox was employed to test the recovery performance because of its stimulation to anammox bacteria. Batch test indicated simultaneous addition of 1.83 ~ 9.17 mg N /L NH2OH relieved Cr(VI) inhibition because of extracellular reduction to Cr(III). The recovery efficiency (RE) was over 166%, with the effluent Cr(VI) and Cr(III) below 0.25 and 0.12 mg/L, respectively. Anammox activity after Cr(VI) inhibition was effectively recovered by 8 mg N/L NH2OH with RE at 218%. The long-term operation showed 1 ~ 2 mg N/L NH2OH accelerated the recover speed of nitrogen removal rate with 2.84 folds, as well as improving NH4+ conversion ratio and reducing NO3− production. After 55 days recovery, extracellular polymeric substance concentration, anammox activity and heme content recovered better with NH2OH addition. This study will provide the theoretical basis for rapid recovery of anammox activity by NH2OH when suffering Cr(VI) inhibition.

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Topics: Anammox (61%)
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5 results found


Journal ArticleDOI: 10.1016/J.BIORTECH.2021.125240
Abstract: The discovery of anammox process has provided eco-friendly and low-cost means of treating ammonia rich wastewater with remarkable efficiency. Furthermore, recent studies have shown that the possibility of operating the anammox process under low temperatures and high organic matter contents broadening the application of the anammox process. However, short doubling time and extensive levels of sensitivity towards nutrients and environmental alterations such as salinity and temperature are the limitations in practical applications of the anammox process. This review article provides the recent yet comprehensive viewpoint on anammox bacteria and the key perspectives in applying them as an efficient strategy for wastewater treatment.

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Topics: Anammox (68%)

7 Citations


Journal ArticleDOI: 10.1016/J.BIORTECH.2021.125108
Qi Song1, Sun Zheng, Yong Chang, Weifeng Zhang  +7 moreInstitutions (1)
Abstract: Polyacrylate containing wastewater (PCW) is the typical sewage discharged by the textile industry. It has extremely poor biodegradability, and chemical methods were used conventionally as the only way for treating PCW. This study is demonstrating a novel biological method. In batch experiment monod kinetics was applied to the experimental data, which indicated that anaerobic treatment used for PCW is feasible. The pilot-scale experiment combined a Spiral Symmetry Stream Anaerobic Bioreactor (SSSAB) and an air-lift external circulation vortex enhancement nitrogen removal fluidized bed bioreactor (AFB). The COD and NH4+-N removal reached up to 95.2% and 96.6%, respectively, which were higher than the value obtained by other chemical methods. High-throughput sequencing analysis indicated that the relative abundance of Proteobacteria, Firmicutes and Bacteroidetes increased, which contribute to the degradation of PCW. Therefore, PCW can be degraded efficiently by using a SSSAB-AFB technique and thus provides an alternative to the chemical methods.

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Topics: Bioreactor (55%), Wastewater (50%)

2 Citations


Journal ArticleDOI: 10.1016/J.SCITOTENV.2021.149449
Abstract: The use of the anammox process for nitrogen removal has gained popularity across the world due to its low energy consumption and waste generation. Anammox reactors have been used to treat ammonium-rich effluents such as chemical, pharmaceutical, semiconductor, livestock, and coke oven wastewater. Recently, full-scale installations have been implemented for municipal wastewater treatment. The efficiency of biological processes is susceptible to inhibitory effects of pollutants present in wastewater. Considering the increasing number of emerging contaminants detected in wastewater, the impacts of the different types of pollutants on anammox bacteria must be understood. This review presents a compilation of the studies assessing the inhibitory effects of different wastewater pollutants towards anammox activity. The pollutants were classified as antibiotics, aromatics, azoles, surfactants, microplastics, organic solvents, humic substances, biodegradable organic matter, or metals and metallic nanoparticles. The interactions between the pollutants and anammox bacteria have been described, as well as the interactions between different pollutants leading to synergistic effects. We also reviewed the effects of pollutants on distinct species of anammox bacteria, and the main toxicity mechanisms leading to irreversible loss of anammox activity have been identified. Finally, we provided an analysis of strategies to overcome the inhibitory effects of wastewater pollutants on the nitrogen removal performance. We believe this review will contribute with essential information to assist the operation and design of anammox reactors treating different types of wastewaters.

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Topics: Anammox (63%), Industrial wastewater treatment (53%), Wastewater (53%) ... read more

1 Citations


Journal ArticleDOI: 10.1016/J.JHAZMAT.2021.126155
Shuang Yang1, Dedong He2, Dedong He1, Liming Zhang1  +3 moreInstitutions (2)
Abstract: Removal of toxic Cr (VI) from aqueous solutions using silicon-based adsorbents has been widely investigated. Meanwhile, contradictory between highly dispersed active Cr species and high Cr loading over commercial Cr-based catalyst was inevitable. In this work, amino-assisted electrostatic adsorption from toxic Cr (VI) treatment was developed to prepare highly dispersed Cr oxides catalysts supported on MCM-41. The Cr loading was as high as 15 wt%, and structure characters of the catalysts were well-reserved. As a result, electrostatic adsorption and subsequent complexation from negatively charged Cr (VI) species and positively charged ammonium groups made a positive contribution to the appearance of highly dispersed mono Cr species, which gave rise to improved non-oxidative propane dehydrogenation (PDH) activity. In contrast, the agglomeration of Cr species and lower PDH activity were observed on the sample synthesized using the traditional wet impregnation method. Besides, the transformation of Cr (VI) to active Cr (III) sites over the catalyst was proved by the designed in-situ H2-TPR, ex-situ UV–vis and Raman spectra results. This procedure reflects a new avenue of green chemistry, which can recycle waste Cr adsorbents as efficient PDH catalysts.

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Topics: Chromium (51%), Adsorption (51%), Catalysis (50%)

Journal ArticleDOI: 10.1016/J.JHAZMAT.2021.126736
Zhang Xingxing1, Xiaonong Zhang1, Peng Wu1, Liping Ma2  +5 moreInstitutions (2)
Abstract: Nitrogen removal from mainstream wastewater via DEnitrifying AMmonium OXidation (DEAMOX) is often challenged by undulated actual temperature and high loading rate. Here, we discovered NH2OH addition (HA) and bio-augmentation (BA) tactics on start-up and operation performance of DEAMOXs (R1 and R2) under ambient temperature (11.3–31.7 °C). Over 340-day operation suggested that R2 received 10 mg/L HA and 1:25 BA ratio (v/v, anammox/partial denitrification sludge) achieved desirable nitrogen removal efficiency (NRE) of 97.22% after 145-day, while R1 under higher BA ratio of 1:12.5 without HA obtained lower NRE (90.86%) after 184-day. Batch tests revealed that nitrate-nitrite transformation ratio reached 98.64% at low COD/NO3-–N of 2.6 with HA. Significantly, compared with R2, R1 recovered quickly with satisfactory effluent total nitrogen of 4.21 mg/L despite nitrogen loading rate greater than 0.15 kg N/m3/d and temperature decreased to 14.6 °C. The abundant narG represented high nitrate reduction potential, hzsA and hdh were extensively detected as the symbolisation of anammox metabolism. Thauera, Denitratisoma and unclassified f Comamonadaceae dominated nitrite accumulation. Ca. Brocadia as the dominant anammox bacteria, and its population maintained stable against low temperature and load shocks by NH2OH intensification. Overall, this study offers an opportunity for the wide-applications of DEAMOX treating mainstream wastewater.

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Topics: Anammox (61%), Denitrification (56%), Denitrifying bacteria (54%) ... read more
References
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31 results found


Open accessJournal ArticleDOI: 10.1128/AEM.65.7.3248-3250.1999
Marc Strous1, J.G. Kuenen, Jetten1Institutions (1)
Abstract: The physiology of anaerobic ammonium oxidizing (anammox) aggregates grown in a sequencing batch reactor was investigated quantitatively. The physiological pH and temperature ranges were 6.7 to 8.3 and 20 to 43°C, respectively. The affinity constants for the substrates ammonium and nitrite were each less than 0.1 mg of nitrogen per liter. The anammox process was completely inhibited by nitrite concentrations higher than 0.1 g of nitrogen per liter. Addition of trace amounts of either of the anammox intermediates (1.4 mg of nitrogen per liter of hydrazine or 0.7 mg of nitrogen per liter of hydroxylamine) restored activity completely.

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Topics: Anammox (70%), Anammoxosome (60%), Ammonium (56%) ... read more

1,123 Citations


Journal ArticleDOI: 10.1016/J.WATRES.2010.08.018
Chong-Jian Tang1, Ping Zheng1, Caihua Wang1, Qaisar Mahmood2  +4 moreInstitutions (2)
01 Jan 2011-Water Research
Abstract: The performance of high-loaded anaerobic ammonium oxidizing (ANAMMOX) upflow anaerobic sludge bed (UASB) reactors was investigated. Two ANAMMOX reactors (R1 with and R2 without effluent recycling, respectively) were fed with relatively low nitrite concentration of 240 mg-N L(-1) with subsequent progressive increase in the nitrogen loading rate (NLR) by shortening the hydraulic retention time (HRT) till the end of the experiment. A super high-rate performance with nitrogen removal rate (NRR) of 74.3-76.7 kg-N m(-3) day(-1) was accomplished in the lab-scale ANAMMOX UASB reactors, which was 3 times of the highest reported value. The biomass concentrations in the reactors were as high as 42.0-57.7 g-VSS L(-1) with the specific ANAMMOX activity (SAA) approaching to 5.6 kg-N kg-VSS(-1) day(-1). The high SAA and high biomass concentration were regarded as the key factors for the super high-rate performance. ANAMMOX granules were observed in the reactors with settling velocities of 73-88 m h(-1). The ANAMMOX granules were found to contain a plenty of extracellular polymers (ECPs) such as 71.8-112.1 mg g-VSS(-1) of polysaccharides (PS) and 164.4-298.2 mg g-VSS(-1) of proteins (PN). High content of hemachrome (6.8-10.3 μmol g-VSS(-1)) was detected in the ANAMMOX granules, which is supposed to be attributed to their unique carmine color.

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Topics: Anammox (68%)

423 Citations


Journal ArticleDOI: 10.1016/J.WATRES.2011.11.024
Eleni Vaiopoulou, Petros Gikas1Institutions (1)
01 Mar 2012-Water Research
Abstract: Chromium is a heavy metal of commercial importance, thus significant amounts are released in wastewaters. Chromium in wastewaters and in the aquatic environment is primarily encountered in oxidation stages +3 (Cr (III) ) and +6 (Cr (VI) ). Recent publications suggest that Cr (VI) compounds are more toxic than Cr (III) ones, while Cr (III) has been identified as trace element, at least for complex organisms. With respect to chromium species mobility, Cr (VI) can cross cellular membranes, which then may be oxidized to Cr (III) and react with intracellular biomolecules. Clear conclusions cannot be derived about the critical chromium concentrations that affect activated sludge growth, as the latter is a function of a number of factors. Broadly, may be supported that activated sludge growth is stimulated at Cr (III) concentrations up to 15 mg L −1 , above which is inhibited, with lethal doses lying above 160 mg Cr (III) L −1 . On the other hand, literature data on Cr (VI) effects on activated sludge are even more controversial. A number of reports support that Cr (VI) is toxic to activated sludge at concentrations above 5 mg L −1 , while others report growth stimulation at concentrations up to 25 mg L −1 . However, all reports agree that Cr (VI) is definitely an activated sludge growth inhibitor at higher concentrations, while 80 mg Cr (VI) L −1 have been identified as lethal dose. A number of factors have been identified to influence chromium toxicity on activated sludge, such as, pH, biomass concentration, presence of organic substances or other heavy metals, acclimation process, exposure time, etc. Naturally, the presence of chromium species in wastewaters may affect the performance of wastewater treatment plants often causing malfunctions, particularly for industrial wastewaters containing relatively high chromium concentrations. The present work reviews in a critical way the published literature on chromium effects on activated sludge, and on the operation of wastewater treatment plants.

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Topics: Activated sludge (58%), Chromium toxicity (56%), Chromium (55%) ... read more

171 Citations


Open accessJournal ArticleDOI: 10.1128/AEM.70.6.3785-3788.2004
Abstract: Anaerobic ammonium oxidation (anammox) is the oxidation of ammonium with nitrite as the electron acceptor and dinitrogen gas as the product (for reviews, see references 7 and 8). The process is mediated by obligately anaerobic chemolithoautotrophic bacteria that form a monophyletic cluster inside the Planctomycetales, one of the major divisions of the Bacteria. So far, four species have been detected and enriched from the biomass of sewage treatment plants: Candidatus “Brocadia anammoxidans” (18), Candidatus “Kuenenia stuttgartiensis” (13), Candidatus “Scalindua wagneri,” and Candidatus “Scalindua brodae” (14). Candidatus “Scalindua sorokinii” was detected in the anoxic water column of the Black Sea (9), providing the first direct evidence for anammox bacteria in the natural environment. Anammox bacteria have a cell compartment known as the anammoxosome, which is the site of anammox catabolism. The lipid bilayer membrane surrounding this anammoxosome contains unusual lipids, so-called “ladderane” lipids, concatenated cyclobutane moieties that are either ether and/or ester linked to the glycerol backbone or occur as free alcohols (e.g., Fig. ​Fig.1,1, structures II to IV) (16). The other membranes of anammox bacteria contain lipids typical for planctomycetes in general: iso, normal, and mid-chain methyl hexadecanoic acids (e.g., Fig. ​Fig.1,1, structure I). FIG. 1. Structure of anammox lipids, i.e., branched fatty acids (I), ladderane fatty acids (II), ladderane glycol ether (III), ladderane glycerol ether (IV), and hop-17(21)-ene (V), present in the enrichment culture of Candidatus “Brocadia anammoxidans.” ... Anammox bacteria have been shown to be chemoautotrophic organisms (17), but it is still unclear which carbon fixation pathway they use. There are currently four known pathways for CO2 fixation in microorganisms (see, e.g., references 2 and 3). The Calvin cycle, with ribulose bisphosphate carboxylase as a key enzyme, is operative in many organisms. The 3-hydroxypropionate pathway has been observed in Chloroflexus aurantiacus and some archaea. The reverse citric acid cycle, with citrate lyase as a key enzyme, has been found in some sulfate-reducing bacteria and phototrophic bacteria. Finally, the acetyl coenzyme A (acetyl-CoA) pathway, with carbon monoxide dehydrogenase/acetyl-CoA synthase as the indicative enzyme, is detected in many anaerobic microorganisms. In addition to enzyme activities, stable carbon isotopic compositions of total cell material and individual lipids, carbohydrates, and amino acids are often used to infer these biosynthetic pathways in organisms, as the fractionation from the inorganic carbon source to the autotrophic biomass in 13C depends on the biosynthetic pathway used (1, 19, 20, 21). Here, we determined enzyme activities and studied the stable carbon isotopic fractionations of Candidatus “Brocadia anammoxidans” to investigate its carbon fixation pathway. Since this bacterium can be grown only in enrichment cultures, and bulk cell material is thus not solely derived from anammox bacteria, we also determined the isotopic compositions of the specific lipids of this bacterium. Furthermore, the isotopic compositions of ladderane lipids derived from Candidatus “Scalindua sorokinii” growing in the anoxic water column of the Black Sea (9) were determined in order to examine the 13C fractionation patterns of anammox bacteria under natural conditions.

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Topics: Anammoxosome (65%), Anammox (64%), Ladderane (63%) ... read more

153 Citations


Journal ArticleDOI: 10.1016/J.WATRES.2015.02.042
Yu Miao1, Run-Hua Liao2, Xu-Xiang Zhang1, Yuan Wang  +4 moreInstitutions (2)
01 Jun 2015-Water Research
Abstract: In this study, a lab-scale expanded granular sludge bed reactor was continuously operated to treat high-nitrate wastewater containing different concentrations of hexavalent chromium (Cr(VI)). Nearly complete nitrate removal was achieved even at 120 mg/L influent Cr(VI). Pyrosequencing of 16S rRNA gene showed that Cr(VI) decreased the biodiversity of the bacterial community and potential denitrifiers. Proteobacteria dominated in the bioreactor, and Betaproteobacteria had increased abundance after Cr(VI) feeding. Thauera and Halomonas were the two predominant genera in the bioreactor fed with Cr(VI), demonstrating opposite responses to the Cr(VI) stress. Metagenomic analysis indicated that Cr(VI) feeding posed no obvious effect on the overall function of the bacterial community, but altered the abundance of specific denitrifying genes, which was evidenced by quantitative real time PCR. This study revealed that Halomonas mainly contributed to the denitrification under no or low Cr(VI) stress, while Thauera played a more important role under high Cr(VI) stress.

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Topics: Halomonas (50%), Denitrifying bacteria (50%), Thauera (50%)

137 Citations