Id2 expression delineates differential checkpoints in the genetic program of CD8α+ and CD103+ dendritic cell lineages.
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Cites result from "Id2 expression delineates different..."
...CD11c+ DCs were Id2lo (Figure S2B), a finding consistent with a role of Id2 for the development of DC subsets (Hacker et al., 2003; Jackson et al., 2011)....
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...CD11c DCs were Id2 (Figure S2B), a finding consistent with a role of Id2 for the development of DC subsets (Hacker et al., 2003; Jackson et al., 2011)....
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...As previously reported (Jackson et al., 2011), B cells (Figure 3A) and most mononuclear phagocytes (Figure S2B) did not express appreciable amounts of Id2....
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657 citations
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References
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"Id2 expression delineates different..." refers background in this paper
...Langerhans cells (LCs) and the dermal DCs are found in skin while migratory tissue-derived CD103þ DCs originate from cutaneous and mucosal tissues such as the lamina propria of respiratory and gastrointestinal tracts (reviewed in Geissmann et al (2010))....
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...DCs can be categorized into a number of different subsets that largely reflect the pattern of expression of cell surface molecules and functional specializations (Geissmann et al, 2010; Steinman and Idoyaga, 2010)....
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2,715 citations
Additional excerpts
...1+OT-I CD8+ T cells CFSE Itgae –/–C57BL/6H-2bm-1 0 120 E 98.5 98.0 5.4 0.26 99.7 82.5 17.5 85 15 0 909 84 16 10.6 89.4 12.5 87.5 0 512 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105102 103 104 105102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 76.7 23.3 8.38 91.6 D CFSE CD45RA–Sirp-α+ DCs CD45RAintSirp-α– DCs OT-II CD4+ T cells OT-I CD8+ T cells alone OT-II CD4+ T cells alone CD103– DCs CD103+ DCs OT-I CD8+ T cells CD103 5....
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...Antigen presentation to naive T cells in vitro In all, 2–2.5 104 purified DC subsets were washed and resuspended in 200ml mouse tonicity complete RPMI 1640 medium containing 2 105 OVA-coated irradiated bm1 splenocytes and 1 105 CFSE-labelled OT-I or OT-II T cells....
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...The presentation of OVA to CFSE-labelled OVA-specific CD4þ T cells (OT-II cells) was used as a control to determine that all populations of DCs could present OVA antigens....
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...CH-2bm-1 (bm1), OT-I (Hogquist et al, 1994), OT-II (Barnden et al, 1998), Batf3 / (Hildner et al, 2008), Irf-8 / (Holtschke et al, 1996) and Itgae / (Schon et al, 2000) mice were used at 6–8 weeks....
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...DCs were purified and then co-cultured with OVA-coated bm-1 splenocytes (which cannot present antigen on H-2Kb) and analysed for their capacity to induce proliferation of CFSE-labelled OVA-specific CD8þ T cells (OT-I cells)....
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1,701 citations
"Id2 expression delineates different..." refers background or result in this paper
...…reveals overlapping but non-redundant roles of Irf-8 and Batf3 during CD8aþ DC and CD103þ DC differentiation Id2, Irf-8 and Batf3 are transcription factors implicated in the development of CD103þ and CD8aþ DCs (Schiavoni et al, 2004; Hildner et al, 2008; Ginhoux et al, 2009; Edelson et al, 2010)....
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...This is consistent with earlier observations of reduced Sirp-a DCs following Flt3L culture (Hildner et al, 2008) but notably when we enumerated the Sirp-a splenic precursor cells earlier in culture, they were not significantly impaired....
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...Batf3 is required for the generation of LN-derived DCs of the CD103þ and CD8aþ lineages (Hildner et al, 2008; Edelson et al, 2010)....
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...…Irf-4 (Suzuki et al, 2004) and Irf-8 (Schiavoni et al, 2002, 2004; Tamura et al, 2005), PU.1 (Carotta et al, 2010), Ikaros, Gfi-1 (Rathinam et al, 2005), Batf3 (Hildner et al, 2008) and signal transducer and activator of transcription (Stat)-3 and Stat-5 (Wu and Liu, 2007; Merad and Manz, 2009)....
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...…led to a model in which conventional CD8aþ DCs and CD103þ DCs are thought to be developmentally related and possess similar functional and localization characteristics as both DC subsets are absent in mice lacking Id2, Irf-8 or Batf3 (Hildner et al, 2008; Ginhoux et al, 2009; Edelson et al, 2010)....
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1,462 citations
Additional excerpts
...CH-2bm-1 (bm1), OT-I (Hogquist et al, 1994), OT-II (Barnden et al, 1998), Batf3 / (Hildner et al, 2008), Irf-8 / (Holtschke et al, 1996) and Itgae / (Schon et al, 2000) mice were used at 6–8 weeks....
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...Antigen presentation to naive T cells in vitro In all, 2–2.5 104 purified DC subsets were washed and resuspended in 200ml mouse tonicity complete RPMI 1640 medium containing 2 105 OVA-coated irradiated bm1 splenocytes and 1 105 CFSE-labelled OT-I or OT-II T cells....
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...The presentation of OVA to CFSE-labelled OVA-specific CD4þ T cells (OT-II cells) was used as a control to determine that all populations of DCs could present OVA antigens....
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...1+OT-I CD8+ T cells CFSE Itgae –/–C57BL/6H-2bm-1 0 120 E 98.5 98.0 5.4 0.26 99.7 82.5 17.5 85 15 0 909 84 16 10.6 89.4 12.5 87.5 0 512 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105102 103 104 105102 103 104 105 102 103 104 105 102 103 104 105 102 103 104 105 76.7 23.3 8.38 91.6 D CFSE CD45RA–Sirp-α+ DCs CD45RAintSirp-α– DCs OT-II CD4+ T cells OT-I CD8+ T cells alone OT-II CD4+ T cells alone CD103– DCs CD103+ DCs OT-I CD8+ T cells CD103 5....
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1,356 citations
"Id2 expression delineates different..." refers background in this paper
...DC subsets in the human blood can be distinguished by their expression of the surface molecules BDCA-1 (CD1c), BDCA-2 (CD303) and BDCA-3 (CD141) (Dzionek et al, 2000)....
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...XCR1, a chemokine receptor expressed on murine splenic CD8aþ DCs (Dorner et al, 2009) together with CD103þ DCs, appears also to define the CD11cþCD141þ human homologues of mouse CD8aþ DCs (Bachem et al, 2010; Crozat et al, 2010; Jongbloed et al, 2010; Poulin et al, 2010)....
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