Journal ArticleDOI
Identification of a male-specific amplified fragment length polymorphism (AFLP) marker in Broussonetia papyrifera
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TLDR
The present study exhibits amplified fragment length polymorphism (AFLP) molecular marker for sex identification in Broussonetia papyrifera based on nine selective amplification primer combinations and indicates that common homology sequence is existed in both male and female plants.Abstract:
The present study exhibits amplified fragment length polymorphism (AFLP) molecular marker for sex identification in Broussonetia papyrifera . Based on nine selective amplification primer combinations, 230 bands were produced and the E -AGG/M-CAA combination was found to be a male-specific AFLP marker. Subsequently, this male-specific AFLP fragment was sequenced and converted into a sequence tagged site (STS) marker. Based on STS sequence, two primers, MADB-1 and MADB-2 (Male-Associated DNA from B. papyrifera ), were designed to verify the specificity of the fragment. The results indicate that common homology sequence is existed in both male and female plants while one of the bands amplified via MADB-2 primer was solely present in male individuals at high annealing temperature up to 66°C. Finally, MADB-2 primer was introduced to amplify another 16 plants and it revealed that this primer could be used as a convenient, efficient, reliable, and low-cost molecular marker for sex identification in B. papyrifera . Keywords: Broussonetia papyrifera , AFLP, STS, male-specific markerread more
Citations
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Journal ArticleDOI
Review on different mechanisms of sex determination and sex-linked molecular markers in dioecious crops: a current update
TL;DR: The present review emphasizes the mode of sex determination among dioecious plants vis-a-vis summarizes the works related to gender specific markers generated using male and female plants from agriculturally important dioemious crops.
Journal ArticleDOI
Sex Distribution of Paper Mulberry (Broussonetia papyrifera) in the Pacific.
Johany Peñailillo,Gabriela Olivares,Ximena Moncada,Claudia Payacán,Chi-Shan Chang,Kuo-Fang Chung,Peter J. Matthews,Andrea Seelenfreund,Daniela Seelenfreund +8 more
TL;DR: Most paper mulberry plants now present in the Pacific appear to be descended from female clones introduced prehistorically, with the presence of male and female plants in Near and Remote Oceania thought to reflect a dual origin.
Journal ArticleDOI
Generation and validation of unique male sex-specific sequence tagged sites (STS) marker from diverse genotypes of dioecious Jojoba-Simmondsia chinensis (Link) Schneider
TL;DR: Two unique ISSR markers, viz.
Journal ArticleDOI
De Novo assembly of expressed transcripts and global transcriptomic analysis from seedlings of the paper mulberry (Broussonetia kazinoki x Broussonetia papyifera).
TL;DR: This global expression analysis provided novel insights about the molecular mechanisms of the biosynthesis of flavonoid, lignin and cellulose, as well as on the response to biotic and abiotic stresses including the remediation of contaminated soil by the paper mulberry.
Journal ArticleDOI
Phylogeography of herbarium specimens of asexually propagated paper mulberry [Broussonetia papyrifera (L.) L'Hér. ex Vent. (Moraceae)] reveals genetic diversity across the Pacific.
Claudia Payacán,Ximena Moncada,Gloria Rojas,Andrew Clarke,Kuo-Fang Chung,Robin G. Allaby,Daniela Seelenfreund,Andrea Seelenfreund +7 more
TL;DR: Insight is gained into the dispersal of paper mulberry into Oceania through the genetic analysis of herbaria samples which represent a more complete coverage of the historical geographical range of the species in the Pacific before later introductions and local extinctions occurred.
References
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Journal Article
Broussonetia papyrifera (l.) l'hér. ex vent.: an environmental constraint on the himalayan foothills vegetation
TL;DR: In this paper, the influence of Broussonetia papyrifera on the native scrub forest at the Himalayan Foothills, Islamabad, Pakistan and its relationship with ecological gradients which are important in terms of its spatial distribution.
Journal ArticleDOI
Identification of a male-specific AFLP marker in a functionally dioecious fig, Ficus fulva Reinw. ex Bl. (Moraceae)
TL;DR: A male-specific amplified fragment length polymorphism (AFLP) marker was identified in the functionally dioecious fig species, Ficus fulva, and sequenced and converted into a sequence characterised amplified region (SCAR) marker, suggesting that sequence divergence between male- and female-specific chromosomal regions is low.
Journal ArticleDOI
Construction of a genetic map and development of DNA markers linked to the sex-determining locus in the Patagonian pejerrey (Odontesthes hatcheri).
TL;DR: A AFLP marker tightly linked to the sex-determining locus provides genetic confirmation that the sex of Patagonian pejerrey is determined genetically and would be useful for the analysis of the molecular basis of GSD and TSD in this species.
Journal ArticleDOI
Isolation of sex-specific AFLP markers in Spotted Halibut (Verasper variegatus)
TL;DR: A reliable AFLP-based genetic sexing of Spotted Halibut that could be useful for genetic mapping of the sex chromosomes and identification of sex-linked genes is provided.
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