Identification of group A rotavirus gene 4 types by polymerase chain reaction.
Citations
598 citations
Cites methods from "Identification of group A rotavirus..."
...The 2 outer capsid proteins carry rotavirus serotype (neutralization)– specific antigens and are encoded by segments 4 (VP4 protease- sensitive protein and P serotype antigen) and by segments 7, 8, or 9 (VP7 glycoprotein and G serotype antigen)....
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...In the case of strain 116E, the VP4 gene is highly related to the bovine serotype P8[11]G10 VP4 gene, whereas the remaining 10 genes are related to typical HRV strains of the Wa genogroup (e.g., P1A[8]G1, SG II, long electrophero- type) [121]; strain I321 (P[11]G10) contains 2 genes from HRVs of the Wa genogroup, and its remaining genes are from a bovine rotavirus [152]....
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...Because the VP4 and VP7 proteins are encoded by separate gene segments, rotaviruses can generate new P-G serotype antigen combina- tions through reassortment after dual infection of single cells....
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...Because identification of P serotypes is technically difficult, usually, only the corresponding VP4 genes are identified by genotyping methods in surveillance stud- ies....
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...Nucleotide sequencing studies provided strong evidence that reassortment occurs between circulating genotype P[8] strains with G1, G3, G4, or G9 specificity when it was found that phylogenetically distinct VP4 genes of such strains can seg- regate with VP7 genes of 11 G serotype [138, 139]....
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459 citations
Cites background or methods from "Identification of group A rotavirus..."
...amplification were identical to those described previously, except that 40 PCR cycles were used and the extension time was 3 min at 720C (8)....
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...As a result, nucleic acid-based (genotyping) methods that detect genetically distinct VP4 genes and accurately predict P serotypes have been developed (8)....
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...Rotavirus doublestranded RNA was extracted from cell lysates or fecal specimens, and 5 ,ul of the eluate was analyzed (8)....
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411 citations
Cites background or methods from "Identification of group A rotavirus..."
...Similarly, a 876 bp fragment of the gene encoding VP4 was amplified and sequenced using primers Con2 and Con3 (Gentsch et al., 1992) from 10 rotavirus strains that failed to P-type....
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...2nd round P[4] CTA TTG TTA GAG GTT AGA GTC (nt 474–494) 483 Gentsch et al. (1992) P[6] TGT TGA TTA GTT GGA TTC AA (nt 259–278) 267 Gentsch et al. (1992)...
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...(2000b) P[9] TGA GAC ATG CAA TTG GAC (nt 385–402) 391 Gentsch et al. (1992) P[10] ATC ATA GTT AGT AGT CGG (nt 575–594) 583 Gentsch et al....
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...(2000b) P[9] TGA GAC ATG CAA TTG GAC (nt 385–402) 391 Gentsch et al. (1992) P[10] ATC ATA GTT AGT AGT CGG (nt 575–594) 583 Gentsch et al. (1992) P[11] GTA AAC ATC CAG AAT GTG (nt 305–323) 312 This paper Con-3 As above...
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...Reverse transcription with random primers and G and P typing semi-nested PCRs were performed as described previously ( Gentsch et al., 1992; Gouvea et al., 1990; Iturriza-Gómara et al., 1999)....
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401 citations
Cites methods from "Identification of group A rotavirus..."
...Genotyping was done by reverse transcriptase-polymerase chain reaction (RT-PCR) followed by probe hybridization confirmation of PCR products [39-41, 59, 60]....
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...Genotyping methods have been reported for 6 of 7 HRV P serotypes, for 9 of 10 HRV G serotypes, and for several P and G serotypes identified only in animals [27, 39-44]....
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360 citations
References
232,912 citations
"Identification of group A rotavirus..." refers methods in this paper
...The concentration of rotavirus dsRNA from cell lysates was estimated by comparison with known amounts of dsRNA from purified virions that were analyzed by polyacrylamide gel electrophoresis (PAGE) and silver staining (16, 26)....
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203,017 citations
14,575 citations
"Identification of group A rotavirus..." refers methods in this paper
...1) was chosen for the first amplification because the primers correspond to regions that are highly conserved among HRV strains from VP4 genetic groups 1 to 4, as determined by best fit analysis with the University of Wisconsin Genetics Computer Group sequence analysis program (3) (using sequences found in the GenEMBL data bank)....
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2,699 citations
"Identification of group A rotavirus..." refers background in this paper
...events during amplification of complex nucleic acids (4)....
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1,524 citations