Identification of Nostoc punctiforme akinete-expressed genes using differential display.
Citations
400 citations
Cites background from "Identification of Nostoc punctiform..."
...punctiforme PCC 73102, which is impaired in heterotrophy, akinetes differentiate when the filaments are placed in the dark in the presence of fructos...
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134 citations
Cites background from "Identification of Nostoc punctiform..."
...…error in the outcome of the assay—for discussion on this subject please refer to Bustin (2002) and Ginzinger (2002)—and a survey of the literature reveals that only a few studies which employ this methodology with cyanobacteria have been published (Argueta et al. 2006; Woodger et al. 2003)....
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118 citations
Cites background from "Identification of Nostoc punctiform..."
...NpR4070 was up-regulated in steady-state N2-grown cultures at a significant but low level....
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...and NpR4070) (6) was present in the akinete transcriptome or...
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...Transcription of avaK (NpF5452), encoding an akinete marker protein (6, 48), was enhanced, as was transcription of patA and hetF, whose products are assigned to heterocyst differentiation....
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...None of the four genes determined by differential display to be up-regulated during akinete development (NpF0062, NpF5999, NpF6000, and NpR4070) (6) was present in the akinete transcriptome or in the NH4 -grown transcriptome or proteome....
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...Five genes that are differentially transcribed during akinete differentiation have recently been identified (6, 48)....
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100 citations
Cites background or result from "Identification of Nostoc punctiform..."
...These results are consistent with the expression of avaK in A. variabilis (Zhou and Wolk 2002), with N. punctiforme differential display results for aet ( Argueta et al. 2006 ), and with microarray results from zwf akinetes for nblA and avaK (Campbell et al. 2007)....
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...Argueta et al. (2006) reported the detection of three novel genes involved in akinete differentiation....
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40 citations
Cites background from "Identification of Nostoc punctiform..."
...Here, we showed that low light was the best inducer of akinete formation in Anabaena variabilis and confirmed the earlier finding (Argueta & Summers, 2005; Argueta et al., 2006) that phosphate starvation was the best inducer in N. punctiforme....
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...Here, we showed that low light was the best inducer of akinete formation in Anabaena variabilis and confirmed the earlier finding (Argueta & Summers, 2005; Argueta et al., 2006) that phosphate starvation was the best inducer in N....
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...However, only a few genes involved in akinete formation have been so far identified (e.g. Argueta et al., 2006; Campbell et al., 1996; Leganés et al., 1994, 1998; Lehner et al., 2011; Zhou &Wolk, 2002; see also review by Maldener et al., 2014)....
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References
59 citations
"Identification of Nostoc punctiform..." refers background in this paper
...A second transcriptional regulatory protein, HetR, was shown to be essential for both heterocyst and akinete formation in N. ellipsosporum (Leganes et al., 1994); however, a hetR mutant strain of N. punctiforme was able to form coldresistant akinete-like cells that lacked obvious granulation…...
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...ellipsosporum (Leganes et al., 1994); however, a hetR mutant strain of N....
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51 citations
"Identification of Nostoc punctiform..." refers methods in this paper
...Nostoc punctiforme ATCC 29133 and the zwf mutant UCD 466 (Hagen and Meeks, 2001) were grown in 125 ml Erlenmeyer flasks containing 50 ml of AA/4 supplemented with 5 mM MOPS buffer (pH 7....
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...Nostoc punctiforme ATCC 29133 and the zwf mutant UCD 466 (Hagen and Meeks, 2001) were grown in 125 ml Erlenmeyer flasks containing 50 ml of AA/4 supplemented with 5 mM MOPS buffer (pH 7.5), 2.5 mM NH4Cl, 2.5 mM NaNO3 and 2.5 mM KNO3 for photoautotrophic growth, and 10 mg ml-1 ampicillin for plasmid…...
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49 citations
"Identification of Nostoc punctiform..." refers background in this paper
...Although the germination of akinetes has received much attention (Chauvat et al., 1982; Sutherland et al., 1985; Sili et al., 1994; van Dok and Hart, 1996), the molecular genetic study of akinete induction has been hindered by their normally slow and asynchronous formation....
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48 citations
"Identification of Nostoc punctiform..." refers background in this paper
...variabilis strain bearing the avaK promoter region fused to a GFP transcriptional reporter exhibited high levels of expression in akinetes as compared with heterocysts or vegetative cells, confirming this gene as an akinete-marker gene (Zhou and Wolk, 2002)....
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...To make this an inclusive promoter analysis, avaK, the only previously described akinete-marker gene originally identified in A. variabilis (Zhou and Wolk, 2002), was included as this gene has also been demonstrated to be expressed in akinetes of N. punctiforme (Argueta et al., 2004)....
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...Prior to this report, the only study focusing specifically on identification of genes involved in akinete formation or function utilized a proteomic approach (Zhou and Wolk, 2002)....
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...variabilis (Zhou and Wolk, 2002), was included as this gene has also been demonstrated to be expressed in akinetes of N....
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...An A. variabilis strain bearing the avaK promoter region fused to a GFP transcriptional reporter exhibited high levels of expression in akinetes as compared with heterocysts or vegetative cells, confirming this gene as an akinete-marker gene (Zhou and Wolk, 2002)....
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46 citations
"Identification of Nostoc punctiform..." refers background in this paper
...…not heat-resistant like the endospores of Gram-positive bacteria, cyanobacterial akinetes have been reported to survive 5–7 years of desiccation (Yamamoto, 1975; Sili et al., 1994), months of cold (4°C) dark conditions (Sutherland et al., 1979), and have been isolated from sediments as old as…...
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...Although not heat-resistant like the endospores of Gram-positive bacteria, cyanobacterial akinetes have been reported to survive 5–7 years of desiccation (Yamamoto, 1975; Sili et al., 1994), months of cold (4°C) dark conditions (Sutherland et al....
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