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Identification of Thermophilic Strains from Geothermal Areas in Turkey by using Conventional and Molecular Techniques
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TLDR
It was determined that FAMEs method failed in the identification of thermophilic bacteria at the species level whereas BOX-PCR technique was quite successful in exhibiting genetic differences among thermal isolates.Abstract:
A survey of isolation was practised by obtaining water samples from various thermal facilities in order to find out new types of thermophilic bacteria and to characterize them via phenotypic and genotypic methods. Hence, the test strains were first grouped according to their colonial morphologies and were characterized via conventional (morphological, physiological, biochemical) and molecular [Fatty Acid Methyl Ester profiles (FAME), 16S rRNA sequencing and BOX-PCR] methods. As the result of the research, it was determined that FAMEs method failed in the identification of thermophilic bacteria at the species level whereas BOX-PCR technique was quite successful in exhibiting genetic differences among thermal isolates. On the other hand, as the result of 16S rRNA sequence, 4 of the isolated strains (A7-A10) were determined to resemble Bacillus licheniformis at a rate of ≥97% while 2 of them (A1 and A13) resembled to Anoxybacillus kaynarcensis, 1 of them (A2) to A. flavithermus, 1 of them (A3) to A. rupiensis, 1 of them (A4) to A. thermarum, 1 of them (A5) to A. gonensis, 1 of them (A11) to B. thermoamylovorans,1 of them (A12) to Ureibacillus thermospaericus, 1 of them (A14) to Brevibacillus brevis, 1 of them (A6) to B. thermolactis and again 1 of them resembled to B. thermoruber (Z1) at the same rate. Their amylase and cellulase productions were also examined and analyzed and the enzyme producing isolates that were biotechnologically valuable were presented to be used in the prospective studies.read more
Citations
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Isolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production
TL;DR: All of the isolates of O20 strain were capable of producing industrially valuable enzymes based on screening and most of them could produce at least two of these enzymes.
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Thermotolerant alkaline protease enzyme from Bacillus licheniformis A10: purification, characterization, effects of surfactants and organic solvents
TL;DR: The extracellular thermostable alkaline protease out of A10 strain was purified 1.38-fold with 9.44% efficiency through the ammonium sulfate precipitation-dialysis and DE52 anion exchange chromatography methods and proved to be a serine alkaline protein.
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Magnesite Enrichment with Pseudomonas oryzihabitans Isolated from Magnesite Ore
TL;DR: In this article, bacterial isolates from magnesite quarries in Mersin were tested by plate assay for their ability to decalcify Magnesite A bacterial strain producing the largest clear zones in the plate assay was identified as Pseudomonas oryzihabitans by 16S rDNA-PCR and applied to the mined ore.
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Identification and Characterization of Novel Thermophilic Bacteria from Hot Springs, Erzurum, Turkey.
Ebru Oztas Gulmus,Arzu Görmez +1 more
TL;DR: The results of the study indicated that these bacteria and their enzymes can be used as a source of industrial enzymes.
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New xylanolytic enzyme from Geobacillus galactosidasius BS61 from a geothermal resource in Turkey.
TL;DR: Results indicated that the xylanase of G. galactosidasius BS61 has biotechnological potential in juice clarification due to its stability against metal ions, chemicals and high pH-values.
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