IL13 activates autophagy to regulate secretion in airway epithelial cells
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Cites background from "IL13 activates autophagy to regulat..."
...At baseline, autophagy in AMs is critical for inhibiting spontaneous pulmonary inflammation(11) and is fundamental for airway mucus secretion by airway goblet cells.(12) Autophagydeficient mice (atg5¡/¡ and atg7¡/¡) develop spontaneous sterile lung inflammation, characterized by marked recruitment of inflammatory cells submucosal thickening, goblet cell metaplasia, and increased collagen content....
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...Genetic depletion of ATG5 and ATG14 or pharmacological inhibition of autophagy with 3-MA or bafilomycin A1 (Baf-A1) in epithelial cells treated with IL13 results in less mucus secretion and less CCL26 (eosinophil chemokine) production, demonstrating that autophagy is involved in the activation of the Th2 response in asthma [343]....
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References
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"IL13 activates autophagy to regulat..." refers background in this paper
...Of these, cytokines are increasingly recognized as potent modulators of autophagy, capable of both activation and inhibition during inflammation.(1,2) Cytokine regulation of autophagy may occur as a component of the immune response to pathogens or in noninfectious disorders such as atherosclerosis or malignancy....
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"IL13 activates autophagy to regulat..." refers background or methods in this paper
...C57BL/6 mice deficient in the autophagy gene Atg16l1 were previously described (Atg16l1).(27) Mice were administered PBS (Sigma-Aldrich, P3813) vehicle or recombinant mouse IL33 (Peprotech, 210–33), 1....
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...Autophagy is now shown to be required for secretory pathways in mast cells, bone osteoclasts, intestinal Paneth cells, and colonic goblet cells.(27,28,50-52) Shared or unique utilization of specific autophagy proteins for secretion is yet to be resolved, Figure 5....
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...However, unlike the physiological requirement for goblet cells in the intestine, goblet cell differentiation in the mouse airway must be induced.(27) Airway goblet cell formation can be efficiently induced by airway delivery of IL33, which leads to macrophage and lymphocyte production of IL13....
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1,335 citations
"IL13 activates autophagy to regulat..." refers methods in this paper
...sequences in HEK293T cells using FuGENE 6 (Promega, E2691) as described.(62) Twenty h after transfection, the virus was collected in DMEM/F12 media with 5% fetal bovine serum then passed through a 45-micron filter (Millipore, CE0459)....
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