Immobilization of Enzymes by Polymeric Materials
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TLDR
In this article, the authors reviewed three primary immobilization methods: physical adsorption, covalent binding and entrapment, with their advantages and drawbacks, and included some polymer applications and their derivatives in the immobilization of enzymes.Abstract:
Enzymes are the highly efficient biocatalyst in modern biotechnological industries. Due to the fragile property exposed to the external stimulus, the application of enzymes is highly limited. The immobilized enzyme by polymer has become a research hotspot to empower enzymes with more extraordinary properties and broader usage. Compared with free enzyme, polymer immobilized enzymes improve thermal and operational stability in harsh environments, such as extreme pH, temperature and concentration. Furthermore, good reusability is also highly expected. The first part of this study reviews the three primary immobilization methods: physical adsorption, covalent binding and entrapment, with their advantages and drawbacks. The second part of this paper includes some polymer applications and their derivatives in the immobilization of enzymes.read more
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Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking studies
Katerine da Silva Moreira,André L. B. de Oliveira,Lourembergue Saraiva de Moura Junior,Isamayra Germano de Sousa,Antônio Luthierre Gama Cavalcante,Francisco Simao Neto,Roberta Bussons Rodrigues Valério,Anderson Valério Chaves,Thiago de Sousa Fonseca,Thiago de Sousa Fonseca,Daniel Morais Vieira Cruz,Daniel Morais Vieira Cruz,Gledson Vieira Lima,Gledson Vieira Lima,Gabriel Paixão de Oliveira,Maria Cristiane Martins de Souza,Pierre Basílio Almeida Fechine,Marcos Carlos de Mattos,Aluísio Marques da Fonseca,José C. S. dos Santos,José C. S. dos Santos +20 more
TL;DR: In this paper, the simultaneous co-immobilization by covalent binding of lipase A from Candida antarctica (CALA) and lipase B from CALB in glutaraldehyde activated chitosan (CHI) was optimized using the Taguchi method.
Journal ArticleDOI
Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking studies
TL;DR: In this paper , the simultaneous co-immobilization by covalent binding of lipase A from Candida antarctica (CALA) and lipase B from CALB in glutaraldehyde activated chitosan (CHI) was optimized using the Taguchi method.
Journal ArticleDOI
Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media
Myrto G. Bellou,Elena Gkantzou,Anastasia Skonta,Dimitrios Moschovas,Konstantinos Spyrou,A. K. Avgeropoulos,Dimitrios Gournis,Haralambos Stamatis +7 more
TL;DR: In this article , 3D-printed polylactic acid (PLA) microwell plates or tubular microfluidic reactors were modified with polyethylenimine (PEI) and lipase from Candida antarctica (CALB) covalently immobilized in the interior of each structure.
Journal ArticleDOI
Enzyme co-localisation: Mechanisms and benefits
TL;DR: A brief overview of the main methods developed for enzyme immobilization, co-localisation, and conjugation can be found in this paper , where the authors discuss the effects emerging from bringing two sequential enzymes of a cascade reaction together, particularly when it speeds up reaction velocity.
Journal ArticleDOI
Nanomaterial Constructs for Catalytic Applications in Biomedicine: Nanobiocatalysts and Nanozymes
Angel M. Villalba-Rodríguez,Lidia Yaritza Martínez-Zamudio,Saúl Antonio Hernández Martínez,Jesús Alfredo Rodríguez-Hernández,Elda M. Melchor-Martínez,Elda A. Flores-Contreras,Reyna Berenice González-González,Roberto Parra-Saldívar +7 more
TL;DR: In this paper , the role played by nanomaterials in catalytic applications is analyzed to identify possible research directions toward the development of the field and the achievement of real practicability.
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TL;DR: An overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented and emphasis is placed on relatively recent developments, such as the use of novel supports such as mesoporous silicas, hydrogels, and smart polymers, and cross-linked enzyme aggregates (CLEAs).
Journal ArticleDOI
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TL;DR: Different methods for the immobilization of enzymes are critically reviewed, with emphasis on relatively recent developments, such as the use of novel supports, e.g., mesoporous silicas, hydrogels, and smart polymers, novel entrapment methods and cross-linked enzyme aggregates (CLEAs).