Immobilization of penicillin G acylase onto amino-modified silica hydrogel
14 Jan 2010-Frontiers of Chemical Engineering in China (SP Higher Education Press)-Vol. 4, Iss: 1, pp 87-90
TL;DR: Amino-modified silica hydrogel (N-MSHG) was prepared by simple sol-gel processing via the co-condensation of commercial silica sol with 3-aminopropyltrieoxysilane.
Abstract: Amino-modified silica hydrogel (N-MSHG) was prepared by a simple sol-gel processing via the co-condensation of commercial silica sol with 3-aminopropyltrieoxysilane. Penicillin G acylase (PGA), a model enzyme, was covalently immobilized onto the N-MSHG and then was used for the enzymatic synthesis of amoxicillin. The samples were characterized by Nitrogen sorption analysis, FT-IR and thermal gravimetric analysis (TGA). The results showed that the amino-modified gel was a mesoporous material with an average pore size of 12.64±0.17 nm. The immobilization process was efficient and the immobilized enzyme showed high catalytic efficiency. The yield of the synthesis of amoxicillin in aqueous media was 38% for 2.5 h. This sol-gel preparation is simple and shows prominent potential value in industrial processing.
Citations
More filters
TL;DR: Magnetic hydroxyl microspheres were prepared by suspension polymerization and activated by epoxy chloropropane as mentioned in this paper, which resulted in a 1.194-fold increase in enzyme activity yield as compared to that of untreated PGA.
Abstract: Magnetic hydroxyl microspheres were prepared by suspension polymerization and activated by epoxy chloropropane. The magnetic particles were characterized in terms of chemical composition, particle size and electrophoretic mobility. These epoxy-activated magnetic particles were assessed as a new carries for immobilized penicillin G acylase (PGA) by covalent coupling. The oriented PGA immobilization was achieved by employing the interaction electrostatic repulsion between PGA and magnetic supports through adding the phenyl acetic acid (PAA), which resulted in a 1.194-fold increase in the enzyme activity yield as compared to that of untreated PGA. No activity of immobilized PGA was lost after 20 cycles and about 94.28% enzyme activity was retained at the end of the 80th cycle in the batch reaction system.
1 citations
References
More filters
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
225,085 citations
"Immobilization of penicillin G acyl..." refers methods in this paper
...The amount of enzyme immobilized on the matrix, which was obtained from the difference between the enzyme amount in solution before and after the immobilization measured by the Bradford method [ 15 ], shows that 81% of the initial protein content was covalently immobilized onto the support....
[...]
TL;DR: Characterization and Properties 3928 8.2.1.
Abstract: 5. In Situ Polymerization 3907 5.1. General Polymerization 3907 5.2. Photopolymerization 3910 5.3. Surface-Initiated Polymerization 3912 5.4. Other Methods 3913 6. Colloidal Nanocomposites 3913 6.1. Sol-Gel Process 3914 6.2. In Situ Polymerization 3916 6.2.1. Emulsion Polymerization 3917 6.2.2. Emulsifier-Free Emulsion Polymerization 3919 6.2.3. Miniemulsion Polymerization 3920 6.2.4. Dispersion Polymerization 3921 6.2.5. Other Polymerization Methods 3923 6.2.6. Conducting Nanocomposites 3924 6.3. Self Assembly 3926 7. Other Preparative Methods 3926 8. Characterization and Properties 3928 8.1. Chemical Structure 3928 8.2. Microstructure and Morphology 3929 8.3. Mechanical Properties 3933 8.3.1. Tensile, Impact, and Flexural Properties 3933 8.3.2. Hardness 3936 8.3.3. Fracture Toughness 3937 8.3.4. Friction and Wear Properties 3937 8.4. Thermal Properties 3938 8.5. Flame-Retardant Properties 3941 8.6. Optical Properties 3942 8.7. Gas Transport Properties 3943 8.8. Rheological Properties 3945 8.9. Electrical Properties 3945 8.10. Other Characterization Techniques 3946 9. Applications 3947 9.1. Coatings 3947 9.2. Proton Exchange Membranes 3948 9.3. Pervaporation Membranes 3948 9.4. Encapsulation of Organic Light-Emitting Devices 3948
1,915 citations
"Immobilization of penicillin G acyl..." refers background in this paper
...Silica-based materials have some attractive properties such as high mechanical strength, relatively high thermal and chemical stabilities, low cost and abundance in nature [1– 3]....
[...]
TL;DR: Aerogels form a new class of solids showing sophisticated potentialities for a range of applications, and can develop very attractive physical and chemical properties not achievable by other means of low temperature soft chemical synthesis.
Abstract: In the present review, aerogels designate dried gels with a very high relative pore volume. These are versatile materials that are synthesized in a first step by low-temperature traditional sol-gel chemistry. However, while in the final step most wet gels are often dried by evaporation to produce so-called xerogels, aerogels are dried by other techniques, essentially supercritical drying. As a result, the dry samples keep the very unusual porous texture which they had in the wet stage. In general these dry solids have very low apparent densities, large specific surface areas, and in most cases they exhibit amorphous structures when examined by X-ray diffraction (XRD) methods. In addition, they are metastable from the point of view of their thermodynamic properties. Consequently, they often undertake a structural evolution by chemical transformation, when aged in a liquid medium and/or heat treated. As aerogels combine the properties of being highly divided solids with their metastable character, they can develop very attractive physical and chemical properties not achievable by other means of low temperature soft chemical synthesis. In other words, they form a new class of solids showing sophisticated potentialities for a range of applications. These applications as well as chemical and physical aspects of these materials were regularly detailed and discussed in a series of symposia on aerogels,1-5 the last of them being held in Albuquerque in 2000.6 Reviews were also regularly published, either on both xerogels and aerogels7 or more focused on the applications of aerogels.8-13 The particularly interesting properties of aerogels arise from the extraordinary flexibility of the solgel processing, coupled with original drying techniques. The wet chemistry is not basically different for making xerogels and aerogels. As this common basis has been extensively detailed in recent books,14 it does not need to be reviewed. Compared to traditional xerogels, the originality of aerogels comes from * To whom all correspondence should be addressed. † Institut de Recherches sur la Catalyse. ‡ Laboratoire d’Application de la Chimie à l’Environnement. 4243 Chem. Rev. 2002, 102, 4243−4265
1,773 citations
TL;DR: In this paper, the aerogels modified by amino-substituted organic groups with bulk densities between 0.07 and 0.16 g cm-3 were prepared by sol−gel processing of Si(OEt)4/(MeO)3Si(CH2)3NR‘2 mixtures (NR 2 = NH2 or NHCH2CH2NH2), followed by drying of the wet gels with supercritical CO2.
Abstract: Monolithic silica aerogels modified by amino-substituted organic groups with bulk densities between 0.07 and 0.16 g cm-3 were prepared by sol−gel processing of Si(OEt)4/(MeO)3Si(CH2)3NR‘2 mixtures (NR‘2 = NH2 or NHCH2CH2NH2), followed by drying of the wet gels with supercritical CO2. The Si(OEt)4/(MeO)3Si(CH2)3NR‘2 ratio was varied between 9:1 and 6:4. The ≡Si(CH2)3NR‘2 groups were completely incorporated in the aerogels in each case. Raman spectroscopic investigations showed that the precursors were rapidly consumed upon addition of water to the alkoxysilane mixtures. Gelling was slowed with an increasing portion of (MeO)3Si(CH2)3NR‘2. The structure of the aerogels was investigated by nitrogen sorption, small-angle X-ray scattering, and transmission electron microscopy. The polarity of the inner surface of the aerogels was rather low. The particle radii were in the range of 6.0−6.3 nm, the fractal dimension in the range of 1.3−1.6, and the BET surface area in the range of 300−430 m2 g-1, rather independe...
122 citations
TL;DR: In this paper, a large-pore nanoporous material, SBA-15, was functionalized with organosilanes by co-condensation method in the presence of nonionic triblock copolymer P123 as a template under acidic conditions.
114 citations