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Journal ArticleDOI

Impact of bioaugmentation by compost on the performance and ecology of an anaerobic digester fed with energy crops.

01 Feb 2011-Bioresource Technology (Elsevier)-Vol. 102, Iss: 3, pp 2931-2935
TL;DR: Fluorescence in situ hybridization indicated that probably an archaeal population shift was responsible for the observed stimulations and an addition of compost induced a methanogenic community change towards hydrogenotrophic methanogens.
About: This article is published in Bioresource Technology.The article was published on 2011-02-01. It has received 38 citations till now. The article focuses on the topics: Compost & Hydraulic retention time.
Citations
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Journal ArticleDOI
TL;DR: In-depth analysis of operational and environmental parameters and bacterial community structure indicated the presence of three potential clusters in anaerobic digestion, determined by total ammonia concentration, free ammonia concentration and temperature, and characterized by an increased relative abundance of Bacteroidales, Clostridiales and Lactobacillales, respectively.

259 citations

Journal ArticleDOI
TL;DR: A controlled anaerobic fermentation of beet silage, only initially inoculated with manure, was monitored by the amplified "ribosomal DNA" restriction analysis, finding that species distribution patterns for Firmicutes, Bacteroidetes, Synergistetes and Thermotogae were not clearly linked to process parameters.

96 citations


Cites methods from "Impact of bioaugmentation by compos..."

  • ...The fermentor was inoculated with a mixture of swine manure, sewage sludge and a hot-rot compost suspension as described previously (Krakat et al., 2010c; Neumann and Scherer, 2011)....

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Journal ArticleDOI
TL;DR: A microbial community based prediction strategy for advanced process control is formulated, which results in a clear trade-off between functionally active and redundant microorganisms as primary basis for microbial community organization.
Abstract: Microbial management in anaerobic digestion is mainly focused on physically present and metabolically active species. Because of its complexity and operation near the thermodynamic equilibria, it is equally important to address functional regulation, based on spatial organisation and interspecies communication. Further establishment of the knowledge on microbial communication in anaerobic digestion through quorum sensing and nanowires is needed. Methods to detect centres of concentrated activity, related to the presence of highly active and well-connected species that take a central role in the anaerobic digestion process, have to be optimized. Bioaugmentation could serve as a crucial tool to introduce keystone species that may create or sustain such centres. Functional stability can be maintained by keeping the microbial community active. This results in a clear trade-off between functionally active and redundant microorganisms as primary basis for microbial community organization. Finally, a microbial community based prediction strategy for advanced process control is formulated.

93 citations

Journal ArticleDOI
TL;DR: Amending activated sludge with additional microorganisms able to produce versatile enzymes to enhance biodegradability or “bioaugmentation”, is gradually considered as a possible solution for the recalcitrance of Bio-Refractory Organic Compounds (Bio-ROCs).

92 citations


Additional excerpts

  • ...Some BAs processed with ell defined chemicals [60,65,92,97] (respectively nitrobenzene, -fluorophenol, phenol, and quinoline) considered as industrial astes and other with undefined effluents related to dairy industry 96] and methanogenesis anaerobic process [59,90] are reported n Table 2B....

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  • ...Neumann and Scherer [90] added compost seeding to an anaerobic digester; BA allowed shorter hydraulic retention times (HRTs) and higher methane production....

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  • ...In terms of treated matrices, many publications refer to pollutants in soil [44,47,48,71–87], others refer to water pollutions cases [53,54,59–61,64–70,88–98] and few ones to polluted sediments [55–57,99]....

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  • ...to work at 2–4 shorter [90]...

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Journal ArticleDOI
01 Aug 2017-Anaerobe
TL;DR: This strategy, known as bioaugmentation, has been used for the last 15 years to increase biomethane production and new strategies that could be used or exploited to improve the success of this approach have also been discussed.

92 citations

References
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Book
01 Jan 1991
TL;DR: Isolation and purification of nucleic acids DNA reassociation experiments DNA-rRNA hybridization and methods DNA sequencing in bacterial systematics direct sequence analysis of small RNAs 16S/23S rRNA sequencing the polymerase chain reaction development and application of nucleics acid probes DNA fingerprinting from macromolecules to trees.
Abstract: Isolation and purification of nucleic acids DNA reassociation experiments DNA-rRNA hybridization and methods DNA sequencing in bacterial systematics direct sequence analysis of small RNAs 16S/23S rRNA sequencing the polymerase chain reaction development and application of nucleic acid probes DNA fingerprinting from macromolecules to trees.

5,198 citations

Journal ArticleDOI
TL;DR: Two supplementary versions of probe EUB338 are designed and evaluated for in situ detection of most of those phyla not detected with this probe, which should allow a more accurate quantification of members of the domain Bacteria in future molecular ecological studies.

2,300 citations

Journal ArticleDOI
TL;DR: A combination of fluorescent rRNA-targeted oligonucleotide probes ("phylogenetic stains") and flow cytometry was used for a high resolution automated analysis of mixed microbial populations and could demonstrate a linear correlation between growth rate and probe-conferred fluorescence of Escherichia coli and Pseudomonas cepacia cells.
Abstract: A combination of fluorescent rRNA-targeted oligonucleotide probes ("phylogenetic stains") and flow cytometry was used for a high resolution automated analysis of mixed microbial populations. Fixed cells of bacteria and yeasts were hybridized in suspension with fluorescein- or tetramethylrhodamine-labeled oligonucleotide probes complementary to group-specific regions of the 16S ribosomal RNA (rRNA) molecules. Quantifying probe-conferred cell fluorescence by flow cytometry, we could discriminate between target and nontarget cell populations. We critically examined changes of the hybridization conditions, kinetics of the hybridization, and posthybridization treatments. Intermediate probe concentrations, addition of detergent to the hybridization buffer, and a posthybridization washing step were found to increase the signal to noise ratio. We could demonstrate a linear correlation between growth rate and probe-conferred fluorescence of Escherichia coli and Pseudomonas cepacia cells. Oligonucleotides labeled with multiple fluorochromes showed elevated levels of nonspecific binding and therefore could not be used to lower the detection limits, which still restrict studies with fluorescing rRNA-targeted oligonucleotide probes to well-growing microbial cells. Two probes of different specificities--one labeled with fluorescein, the other with tetramethylrhodamine--could be applied simultaneously for dual color analysis.

1,219 citations

Journal ArticleDOI
TL;DR: The aim of this paper is primarily to review the recent literature about the occurrence of both acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of particulate biomass to methane (not wastewater treatment), while this review does not cover the activity of the acetate oxidizing bacteria.
Abstract: Among different conversion processes for biomass, biological anaerobic digestion is one of the most economic ways to produce biogas from various biomass substrates In addition to hydrolysis of polymeric substances, the activity and performance of the methanogenic bacteria is of paramount importance during methanogenesis The aim of this paper is primarily to review the recent literature about the occurrence of both acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of particulate biomass to methane (not wastewater treatment), while this review does not cover the activity of the acetate oxidizing bacteria Both acetotrophic and hydrogenotrophic methanogens are essential for the last step of methanogenesis, but the reports about their roles during this phase of the process are very limited Despite, some conclusions can still be drawn At low concentrations of acetate, normally filamentous Methanosaeta species dominate, eg, often observed in sewage sludge Apparently, high concentrations of toxic ionic agents, like ammonia, hydrogen sulfide (H2S) and volatile fatty acids (VFA), inhibit preferably Methanosaetaceae and especially allow the growth of Methanosarcina species consisting of irregular cell clumps, eg, in cattle manure Thermophilic conditions can favour rod like or coccoid hydrogenotrophic methanogens Thermophilic Methanosarcina species were also observed, but not thermophilic Methanosaetae Other environmental factors could favour hydrogentrophic bacteria, eg, short or low retention times in a biomass reactor However, no general rules regarding process parameters could be derivated at the moment, which favours hydrogenotrophic methanogens Presumably, it depends only on the hydrogen concentration, which is generally not mentioned in the literature

1,018 citations