Impaired innate interferon induction in severe therapy resistant atopic asthmatic children.
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"Impaired innate interferon inductio..." refers background in this paper
...STRA was defined as persistent (most days, for at least 3 months) chronic symptoms of airway obstruction (requiring a rescue bronchodilator on at least 3 days per week), despite treatment with high dose ICSs (at least 800mg/day of beclomethasone equivalent) and trials of add on drugs (long acting b2 agonists, leukotriene receptor antagonists, and oral theophylline in a low, anti-inflammatory dose) and/or recurrent severe asthma exacerbations.(43) None of the subjects was taking antibiotics at the time of the study and all were considered to be free of clinical infections....
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"Impaired innate interferon inductio..." refers background or methods in this paper
...Recently, the signaling pathway(s) used by RV to induce IFN-b and IFN-l have been studied.17,18,40 RV dsRNA is recognized by TLR3 and the RLHs RIG-I and MDA5....
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...These data suggest that TLR3 expression may be impaired in asthmatic cells, but this observation was not seen in a recent study of asthmatic BAL macrophages.27 The decreased expression of TLR3 in STRA cells may also explain trends for lower CXCL8 protein release, as RV induction of CXCL8 is TLR3 dependent.18 We also saw decreased induction of RIG-I and MDA5 by viruses at 24 h, which we believe is due to a lack of IFN acting as an additional inducer of RIG-I and MDA5....
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...Previously, we established that both RIG-I and MDA5 are polyIC inducible in BECs.18 At 8 h post-treatment, we found that polyIC significantly induced RIG-I mRNA in NANA (Po0....
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...Virus culture, reagents, and treatment of BECs....
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...All primers and probe sequences are previously reported and were used in ratios as previously described.(18,27)...
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280 citations
"Impaired innate interferon inductio..." refers methods in this paper
...Supernatants were thawed and used to infect HeLa cells as previously described.(46) The amount of RV16 or RV1B was assessed as a TCID50 value using the Karber formula....
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