Journal ArticleDOI
Improved automated lactate determination
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TLDR
An accurate, reproducible, simple, and inexpensive method for determination of lactate concentration that utilizes the enzymic conversion of lactates to pyruvate and NADH in the presence of lactic dehydrogenase is presented.About:
This article is published in Analytical Biochemistry.The article was published on 1970-11-01. It has received 94 citations till now.read more
Citations
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Increased rat cardiac angiotensin converting enzyme activity and mRNA expression in pressure overload left ventricular hypertrophy. Effects on coronary resistance, contractility, and relaxation.
Heribert Schunkert,Victor J. Dzau,Victor J. Dzau,Shiow Shin Tang,Alan T. Hirsch,Carl S. Apstein,Beverly H. Lorell +6 more
TL;DR: It is suggested that cardiac ACE is induced in hearts with left ventricular hypertrophy, and that the resultant intracardiac activation of angiotensin II may have differential effects on myocardial relaxation in hypertrophied hearts relative to controls.
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Left ventricular performance, regional blood flow, wall motion, and lactate metabolism during transluminal angioplasty.
P. W. Serruys,William Wijns,M. van den Brand,Simon H. Meij,Cornelis J. Slager,Johan C.H. Schuurbiers,P. G. Hugenholtz,Ronald W. Brower +7 more
TL;DR: The results of this study indicate that there is no permanent dysfunction of global or regionalMyocardial mechanics, myocardial blood flow, or lactate metabolism after PTCA with four to six coronary occlusions of 40 to 60 sec.
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Protective effect of increased glycolytic substrate against systolic and diastolic dysfunction and increased coronary resistance from prolonged global underperfusion and reperfusion in isolated rabbit hearts perfused with erythrocyte suspensions.
TL;DR: The G + I intervention, in clinically relevant concentrations, markedly protected severely underperfused myocardium for 150 minutes and may be a beneficial intervention in combination with reperfusion therapy in acute myocardial infarction.
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Hypoxanthine production by ischemic heart demonstrated by high pressure liquid chromatography of blood purine nucleosides and oxypurines
TL;DR: It is concluded that the high pressure liquid chromatographic assay of blood hypoxanthine is a useful tool in the diagnosis of ischemic heart disease.
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Ventricular contracture and compliance changes with global ischemia and reperfusion, and their effect on coronary resistance in the rat.
TL;DR: A study of the factors affecting the aluminum oxidetrihydroxyindole procedure for the analysis of catecholamines in several tissues and the development of supersensitivity to norepinephrine after pretreatment with reserpine.
References
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Metalloenzymes and myocardial infarction. ii. malic and lactic dehydrogenase activities and zinc concentrations in serum
Journal ArticleDOI
The fluorometric measurement of pyridine nucleotides.
TL;DR: It has been found practicable with a fluorometer to determine separately oxidized and reduced pyridine nucleotides and the useful range of sensitivity is about 2000-fold, which is much greater than that obtained with calorimetry.
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Fluorescence spectra and polarization of glyceraldehyde-3-phosphate and lactic dehydrogenase coenzyme complexes.
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Automated lactic acid determination in serum and tissue extracts.
TL;DR: Methods for assay of lactic acid in tissue extracts or blood specimens through the use of the AutoAnalyzer have been developed, including a direct automation of the color development of the Barker-Summerson procedure.