Improved extraction of chlorophyll a and b from algae using dimethyl sulfoxide
W. Thomas Shoaf,Bruce W. Lium +1 more
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Dimethyl sulfoxide (DMSO) and 90% acetone extracted equal amounts of chlorophyll from diatoms and blue-green algae, but DMSO was superior to 90%acetone for all green algae tested giving 2-60 times more chlorophyLL depending on the species as discussed by the authors.Abstract:
Dimethyl sulfoxide (DMSO) and 90% acetone extracted equal amounts of chlorophyll from diatoms and blue-green algae, but DMSO was superior to 90% acetone for all green algae tested giving 2–60 times more chlorophyll depending on the species. The absorbance spectra of pure chlorophyll a (and b) from 600 nm to 750 nm were identical whether dissolved in 90% acetone or a mixture of DMSO and 90% acetone (1:1 v/v). Thus, several equations for estimating chlorophyll concentration based on extinction in 90% acetone are applicable with this solvent.read more
Citations
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A method for the extraction of chlorophyll from leaf tissue without maceration
J. D. Hiscox,G. F. Israelstam +1 more
TL;DR: A simple, rapid method requiring few manipulations for the extraction of chlorophylls from fragmented leaf tissue of angiosperms and gymnosperms is compared with the widely used acetone method, which makes use of incubation at 65 °C of leaf tissue immersed in dimethyl sulphoxide.
Journal ArticleDOI
Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis
TL;DR: The efficiency of pigment extraction forms the crux of the spectrophotometric analysis of chlorophyll a and the alcoholic solvents, methanol and ethanol, proved to be superior to acetone and acetone with DMSO.
Journal ArticleDOI
A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants
TL;DR: In this article, the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants was reevaluated, and the problem of degradation resulting from the presence of acidic lichen substances was specifically addressed.
Journal ArticleDOI
Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves
TL;DR: The SPAD-502 has been shown to be a good tool to diagnose the integrity of the photosynthetic system in coffee leaves, and can thus help in the advanced interpretations of the photochemical process of these plants.
Journal ArticleDOI
Assessment of the relationships between dominant cell size in natural phytoplankton communities and the spectral shape of the absorption coefficient
TL;DR: It was found that, in general, when phytoplankton abundance increases, larger sizeclasses are added incrementally to a background of smaller cells, such as pigment packaging and concentration of accessory pigments.
References
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The culture collection of algae at indiana university
TL;DR: All cultures in the Collection are available to all interested individuals and organizations, both academic and commercial, and to acadenmic institutions and nonprofit organizations the cost per culture is $2.002 postpaid; commercial organizations are charged $10.00.
OtherDOI
Methods for collection and analysis of aquatic biological and microbiological samples
TL;DR: Chapter A4 contains methods used by the US Geological Survey to collect, preserve, and analyze waters to determine their biological and microbiological properties.
Biological Field And Laboratory Methods For Measuring The Quality Of Surface Waters And Effluents
TL;DR: The role of aquatic biology in the water pollution control program of the U. S. Environmental Protection Agency includes field studies and laboratory studies carried out to establish water quality criteria for the recognized beneficial uses of water resources and to monitor water quality.
Journal ArticleDOI
Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide
TL;DR: An analytical procedure for these pigments has been developed, based upon this extraction sequence, followed by partition of the acetone extract between hexane and aqueous acetone.
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