Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles.
Seon-Ju Yeo,Duong Tuan Bao,Ga-Eun Seo,Cuc Thi Bui,Do Thi Hoang Kim,Nguyen Thi Viet Anh,Trinh Thi Thuy Tien,Nguyen Thi Phuong Linh,Hae-Jin Sohn,Chom-Kyu Chong,Ho-Joon Shin,Hyun Park +11 more
Reads0
Chats0
TLDR
Novel monoclonal antibodies against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system.Abstract:
The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.read more
Citations
More filters
Journal ArticleDOI
Anti-fouling and thermosensitive ion-imprinted nanocomposite membranes based on grapheme oxide and silicon dioxide for selectively separating europium ions.
Jian Lu,Jian Lu,Yilin Wu,Xinyu Lin,Jia Gao,Hongjun Dong,Li Chen,Yingying Qin,Liang Wang,Yongsheng Yan +9 more
TL;DR: The ion-imprinted nanocomposite membranes synthesized in this work have shown great potentials for selective separation of rare earth ions and negligible damage of the membranes after buried for 20 d indicated the superior anti-fouling property of the Eu-IIMs.
Journal ArticleDOI
Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.
Christine F. Markwalter,Andrew G. Kantor,Carson Paige Moore,Kelly A. Richardson,David W. Wright +4 more
TL;DR: This review of works from the past 20 years in which inorganic chemistry and nanotechnology were implemented in each of the core components that make up a diagnostic test highlights the detection of nucleic acids at the point of care as an emerging application of inorganic Chemistry.
Journal ArticleDOI
Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips.
Meng Xiao,Kaixin Xie,Xiaohui Dong,Lei Wang,Caihong Huang,Fei Xu,Wei Xiao,Meilin Jin,Boyan Huang,Yong Tang +9 more
TL;DR: The SERS-based LFIA strip proposed in this study shows tremendous potential to detect targets quickly and sensitively using an elegantly simple method.
Journal ArticleDOI
Avian Influenza in Wild Birds and Poultry: Dissemination Pathways, Monitoring Methods, and Virus Ecology
Artem Blagodatski,Kseniya Trutneva,Olga Glazova,Olga Mityaeva,Liudmila Shevkova,Evgenii Kegeles,Nikita Onyanov,Kseniia Fede,Anna Maznina,Elena Khavina,Seon-Ju Yeo,Hyun Park,Pavel Volchkov +12 more
TL;DR: In this article, the role of migratory birds in the spread and introduction of influenza strains on a global level, based on recent data, is assessed, which sheds light on the details of viral dissemination linked to avian migration, the viral exchange between migratory waterfowl and domestic poultry, virus ecology in general, and viral evolution as a process tightly linked to bird migration.
Journal ArticleDOI
Effective Bioactivity Retention of Low-Concentration Antibodies on HFBI-Modified Fluorescence ICTS for Sensitive and Rapid Detection of PSA.
Bo Zhang,Weichen Gao,Jiafang Piao,Yunjie Xiao,Bin Wang,Weipan Peng,Xiaoqun Gong,Xiaoqun Gong,Zefang Wang,Zefang Wang,Haitao Yang,Jin Chang +11 more
TL;DR: The results indicated that HFBI could self-assemble on the PNM and immobilize the Abs in the "stand-up" orientation and provide a novel and promising strategy of Ab immobilization on FICTS for near-patient and point-of-care application.
References
More filters
Limit of blank, limit of detection and limit of quantitation.
David A Armbruster,Terry Pry +1 more
TL;DR: The Limit of Blank, Limit of Detection, and Limit of Quantitation are terms used to describe the smallest concentration of a measurand that can be reliably measured by an analytical procedure.
Journal ArticleDOI
Development of a Real-Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
Erica Spackman,Dennis A. Senne,T. J. Myers,Leslie L. Bulaga,Lindsey P. Garber,Michael L. Perdue,Kenton L. Lohman,Luke T. Daum,David L. Suarez +8 more
TL;DR: The sensitivity and specificity of the real-time PCR assay were directly compared with those of the current standard for detection of influenza virus: virus isolation in embryonated chicken eggs and hemagglutinin subtyping by hemagGLutination inhibition (HI) assay.
Journal ArticleDOI
Fluorescence microscopy today.
TL;DR: The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues.
Journal ArticleDOI
Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome
Ron A. M. Fouchier,Peter M. Schneeberger,Frans W. Rozendaal,Jan M. Broekman,Stiena A. G. Kemink,Vincent J. Munster,Thijs Kuiken,Guus F. Rimmelzwaan,Martin Schutten,Gerard J. J. van Doornum,Guus Koch,A Bosman,Marion Koopmans,Albert D. M. E. Osterhaus +13 more
TL;DR: Because H7N7 viruses have caused disease in mammals, including horses, seals, and humans, on several occasions in the past, they may be unusual in their zoonotic potential and, thus, form a pandemic threat to humans.
Journal ArticleDOI
Transmission of H7N7 avian influenza A virus to human beings during a large outbreak in commercial poultry farms in the Netherlands
Marion Koopmans,Berry Wilbrink,Marina Conyn,G Natrop,Hans van der Nat,Harry Vennema,Adam Meijer,Jim E. van Steenbergen,Ron A. M. Fouchier,Albert D. M. E. Osterhaus,A Bosman +10 more
TL;DR: An unexpectedly high number of transmissions of avian influenza A virus subtype H7N7 to people directly involved in handling infected poultry, and evidence for person-to-person transmission are noted.