In situ fabrication of metal-organic framework derived hybrid nanozymes for enhanced nanozyme-photothermal therapy of bacteria-infected wounds
TL;DR: In this paper, a metal-organic framework (MOF)-derived hybrid nanozymes antibacterial strategy for enhanced nanozyme-photothermal therapy (NPT) was proposed, which can not only prevent the aggregation of platinum nano-zymes and effectively reduce the mass transfer resistance during the kinetic reaction, but also inhibit the photoelectron-hole recombination in the process of photothermal therapy.
Abstract: In recent years, nanozyme-photothermal therapy (NPT) has attracted enormous interests owing to their enhanced therapeutic effects and less adverse effects in the treatment of infectious diseases. However, the development of nanozyme-photothermal agents (NPAs) that can rapidly, efficiently and synergistically combating pathogenic bacteria remains a huge challenge due to the limitation of size effect. Herein, by decorating platinum nanozymes on Zn-based photosensitizer, we report a novel metal-organic framework (MOF)-derived hybrid nanozymes antibacterial strategy for enhanced NPT. This strategy can not only prevent the aggregation of platinum nanozymes and effectively reduce the mass transfer resistance during the kinetic reaction, but also inhibit the photoelectron-hole recombination in the process of photothermal therapy (PTT) and improve the photothermal conversion performance. In the presence of a low concentration of hydrogen peroxide (H2O2), the superior nanocatalytic activity of the MOF-derived hybrid nanozymes can effectively catalyze the release of H2O2 to generate toxic hydroxyl radical (•OH), resulting in the increase of bacterial membrane permeability and thermal sensitivity. Once the near-infrared laser is introduced, the nanozyme-photothermal antibacterial platform can play the role of “nanoknife” to further induce the death of the damaged bacteria by physical cutting. In vitro and vivo in antibacterial assays confirm that the MOF-derived hybrid nanozymes have excellent antibacterial properties, which can serve as an antibacterial candidate with negligible adverse effect. Therefore, this work will open a new avenue for MOF-derived hybrid nanozymes in biomedical application.
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TL;DR: In this article , a hybrid membranes-coated hollow copper sulfide nanoparticles (D-CuS@NR NPs) were fabricated for osteoarthritis (OA) treatment, which achieved the synergistic treatment of mild heating, prolonged circulation, and targeted delivery in this system.
Abstract: Osteoarthritis (OA), as a chronic degenerative joint disorder, has seriously affected the life quality of patients. Despite lots of drug treatment strategies that have been studied, the therapeutic effect is still unsatisfactory due to the lack of prolonged circulation life and targeted delivery ability. Recently, functional cell membranes modified nanoparticles have been explored to achieve high-efficiency drug delivery. Herein, neutrophil-erythrocyte hybrid membranes-coated dexamethasone sodium phosphate (Dexp)-loaded hollow copper sulfide nanoparticles (D-CuS@NR NPs) were fabricated for OA treatment. Generally, we achieved the synergistic treatment of mild-heating, prolonged circulation, and targeted delivery in this system. In particular, this biomimetic nanoparticle showed significant cytocompatibility and anti-inflammatory ability in vitro due to cell membrane coating and photothermal responsive drug release under NIR irradiation. Importantly, in vivo explorations revealed that D-CuS@NR NPs combined with photothermal treatment obtained an excellent therapy effect for preventing the OA process. Hence, this novel hybrid membranes-coated CuS NPs showed significant therapeutic efficacy by local warming and targeted drug delivery , which might become a promising drug delivery vehicle for improving the therapeutic effect of OA. • This nanoparticle possesses lots of advantages, including long circulation effect, excellent target ability, and well photothermal conversion efficiency. • Combined with NIR treatment, this drug loaded nanoparticle gained outstanding anti-inflammatory effect and ability to protect articular cartilage in vivo . • This drug loaded nanoparticle provides a promising platform for inflammation related diseases.
71 citations
01 Feb 2022
TL;DR: Wang et al. as discussed by the authors used chitosan (CS) and graphene oxide (GO) to form a complex and followed by the introduction of tannic acid (TA) as secondary cross-linking agent after removing the acidity of CS.
Abstract: Skin trauma with uncontrolled bleeding and bacterial infection are major challenges in clinical wound management. Therefore, the design of elastic and biodegradability wound dressing with antibacterial and hemostatic properties are attracting increasing attention. In the study, chitosan (CS) and graphene oxide (GO) are covalently bound by EDC/NHS to form a complex and followed by the introduction of tannic acid (TA) as secondary cross-linking agent after removing the acidity of chitosan. CS/GO/TA sponges can exist stably in aqueous solution and has good compression recovery performance. In vitro experiments showed that CS/GO/TA sponges have antibacterial, antioxidant, non-hemolysis, blood cell adhesion and cytocompatibility. In vivo experiments in rats showed that CS/GO/TA sponges can quickly stop bleeding and promote wound healing, which has good clinical application potential.
39 citations
TL;DR: In this article , an activated biochar-based graphitic carbon nitride composite (ACB-K-gC3N4 composite) was synthesized via the innovative ultrasonic-milling method.
23 citations
TL;DR: It is proposed that nanozybiotics may bear promising applications in antibacterial therapy, due to their high stability, rapid bacterial killing, biofilm elimination, and low cost.
Abstract: Infectious diseases caused by bacteria represent a global threat to human health. However, due to the abuse of antibiotics, drug-resistant bacteria have evolved rapidly and led to the failure of antibiotics treatment. Alternative antimicrobial strategies different to traditional antibiotics are urgently needed. Enzyme-based antibacterials (Enzybiotics) have gradually attracted interest owing to their advantages including high specificity, rapid mode-of-action, no resistance development, etc. However, due to their low stability, potential immunogenicity, and high cost of natural enzymes, enzybiotics have limitations in practical antibacterial therapy. In recent years, many nanomaterials with enzyme-like activities (Nanozymes) have been discovered as a new generation of artificial enzymes and perform catalytic antibacterial effects against bacterial resistance. To highlight the progress in this field of nanozyme-based antibacterials (Nanozybiotics), this review discussed the antibacterial mechanism of action of nanozybiotics with a comparison with enzybiotics. We propose that nanozybiotics may bear promising applications in antibacterial therapy, due to their high stability, rapid bacterial killing, biofilm elimination, and low cost.
15 citations
TL;DR: In this review, the typical preparation strategies, catalytic mechanisms, advances and perspectives of Mn-based nanozymes for biomedical applications are systematically summarized and the application of Mn -based nanoZymes in tumor therapy and sensing detection, together with an overview of their mechanism of action is highlighted.
Abstract: Manganese (Mn) has attracted widespread attention due to its low‐cost, nontoxicity, and valence‐rich transition. Various Mn‐based nanomaterials have sprung up and are employed in diverse fields, particularly Mn‐based nanozymes, which combine the physicochemical properties of Mn‐based nanomaterials with the catalytic activity of natural enzymes, and are attracting a surge of research, especially in the field of biomedical research. In this review, the typical preparation strategies, catalytic mechanisms, advances and perspectives of Mn‐based nanozymes for biomedical applications are systematically summarized. The application of Mn‐based nanozymes in tumor therapy and sensing detection, together with an overview of their mechanism of action is highlighted. Finally, the prospective directions of Mn‐based nanozymes from five perspectives: innovation, activity enhancement, selectivity, biocompatibility, and application broadening are discussed.
6 citations
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TL;DR: In this article, the newly emerging metal-organic frameworks (MOFs) built from metal ions and polyfunctional organic ligands have proved to be promising self-sacrificing templates and precursors for preparing various carbon-based nanomaterials, benefiting from their high surface areas, abundant metal/organic species, large pore volumes, and extraordinary tunability of structures and compositions.
Abstract: Carbon-based nanomaterials have been widely used as catalysts or catalyst supports in the chemical industry or for energy or environmental applications due to their fascinating properties. High surface areas, tunable porosity, and functionalization are considered to be crucial to enhance the catalytic performance of carbon-based materials. Recently, the newly emerging metal–organic frameworks (MOFs) built from metal ions and polyfunctional organic ligands have proved to be promising self-sacrificing templates and precursors for preparing various carbon-based nanomaterials, benefiting from their high BET surface areas, abundant metal/organic species, large pore volumes, and extraordinary tunability of structures and compositions. In comparison with other carbon-based catalysts, MOF-derived carbon-based nanomaterials have great advantages in terms of tailorable morphologies and hierarchical porosity and easy functionalization with other heteroatoms and metal/metal oxides, which make them highly efficient as...
992 citations
TL;DR: This review summarizes recent developments of MOF-enzyme composites with special emphasis on preparative techniques and the synergistic effects of enzymes and MOFs.
Abstract: The ex vivo application of enzymes in various processes, especially via enzyme immobilization techniques, has been extensively studied in recent years in order to enhance the recyclability of enzymes, to minimize enzyme contamination in the product, and to explore novel horizons for enzymes in biomedical applications. Possessing remarkable amenability in structural design of the frameworks as well as almost unparalelled surface tunability, Metal–Organic Frameworks (MOFs) have been gaining popularity as candidates for enzyme immobilization platforms. Many MOF–enzyme composites have achieved unprecedented results, far outperforming free enzymes in many aspects. This review summarizes recent developments of MOF–enzyme composites with special emphasis on preparative techniques and the synergistic effects of enzymes and MOFs. The applications of MOF–enzyme composites, primarily in transferation, catalysis and sensing, are presented as well. The enhancement of enzymatic activity of the composites over free enzymes in biologically incompatible conditions is emphasized in many cases.
915 citations
TL;DR: These adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing and are an excellent wound dressing for full-thickness skin repair.
Abstract: Developing injectable nanocomposite conductive hydrogel dressings with multifunctions including adhesiveness, antibacterial, and radical scavenging ability and good mechanical property to enhance full-thickness skin wound regeneration is highly desirable in clinical application. Herein, a series of adhesive hemostatic antioxidant conductive photothermal antibacterial hydrogels based on hyaluronic acid-graft-dopamine and reduced graphene oxide (rGO) using a H2 O2 /HPR (horseradish peroxidase) system are prepared for wound dressing. These hydrogels exhibit high swelling, degradability, tunable rheological property, and similar or superior mechanical properties to human skin. The polydopamine endowed antioxidant activity, tissue adhesiveness and hemostatic ability, self-healing ability, conductivity, and NIR irradiation enhanced in vivo antibacterial behavior of the hydrogels are investigated. Moreover, drug release and zone of inhibition tests confirm sustained drug release capacity of the hydrogels. Furthermore, the hydrogel dressings significantly enhance vascularization by upregulating growth factor expression of CD31 and improve the granulation tissue thickness and collagen deposition, all of which promote wound closure and contribute to a better therapeutic effect than the commercial Tegaderm films group in a mouse full-thickness wounds model. In summary, these adhesive hemostatic antioxidative conductive hydrogels with sustained drug release property to promote complete skin regeneration are an excellent wound dressing for full-thickness skin repair.
758 citations
TL;DR: Wound healing results indicate that the synergy antibacterial system could be conveniently used for wound disinfection in vivo.
Abstract: We have developed a biocompatible antibacterial system based on polyethylene glycol functionalized molybdenum disulfide nanoflowers (PEG-MoS2 NFs). The PEG-MoS2 NFs have high near-infrared (NIR) absorption and peroxidase-like activity, which can efficiently catalyze decomposition of low concentration of H2O2 to generate hydroxyl radicals (·OH). The conversion of H2O2 into ·OH can avoid the toxicity of high concentration of H2O2 and the ·OH has higher antibacterial activity, making resistant bacteria more vulnerable and wounds more easily cured. The PEG-MoS2 NFs combine the catalysis with NIR photothermal effect, providing a rapid and effective killing outcome in vitro for Gram-negative ampicillin resistant Escherichia coli (Ampr E. coli) and Gram-positive endospore-forming Bacillus subtilis (B. subtilis) as compared to catalytic treatment or photothermal therapy (PTT) alone. Wound healing results indicate that the synergy antibacterial system could be conveniently used for wound disinfection in vivo. Inte...
717 citations
TL;DR: By decorating platinum nanozymes on photosensitizer integrated MOFs, this work reports a simple yet versatile strategy for enhanced PDT that can facilitate the formation of 1O2 in hypoxic tumor site via H2O2-activated evolvement of O2, which can cause more serious damage to cancer cells.
Abstract: Metal–organic frameworks (MOFs) have been used for photodynamic therapy (PDT) of cancers by integrating photosensitizers, which cause cytotoxic effects on cancer cells by converting tumor oxygen into reactive singlet oxygen (1O2). However, the PDT efficiency of MOFs is severely limited by tumor hypoxia. Herein, by decorating platinum nanozymes on photosensitizer integrated MOFs, we report a simple yet versatile strategy for enhanced PDT. The platinum nanoparticles homogeneously immobilized on MOFs possess high stability and catalase-like activity. Thus, our nanoplatform can facilitate the formation of 1O2 in hypoxic tumor site via H2O2-activated evolvement of O2, which can cause more serious damage to cancer cells. Our finding highlights that the composites of nanozymes and MOFs have the potential to serve as efficient agents for cancer therapy, which will open an avenue of nanozymes and MOFs toward biological applications.
582 citations