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Journal ArticleDOI

In vitro antioxidant activity of aqueous and ethanol leaf extracts of rhinacanthus nasutus (linn.) kurz. (acanthaceae)

30 Dec 2018-Vol. 5, Iss: 2, pp 53-55
Abstract: In the present research work was to examine the possible antioxidant activities of the aqueous and ethanol leaf extract of Rhinacanthus nasutus (Linn.) Kurz. DPPH, Hydroxyl radical scavenging and reducing power assays were employed. The results showed that the DPPH activity of aqueous and ethanol leaf extract at the dose of 50μg/ml has exhibited in 63.81±0.013 and 79.36±0.028 inhibition with an IC50 value of 21.39 and 29.41μg/ml. The highest Hydroxyl radical scavenging activity showed aqueous and ethanol leaf extract at the dose of 50μg/ml has exhibited in 96.18±0.029 and 121.23±0.081 inhibition with an IC50 values of 30.19and 41.39μg/ml, the reducing power assay aqueous and ethanol leaf extract showed the 0.59 and 0.71 absorption at 700 nm extract at the dose of 50μg/ml suggested that promising antioxidant activity of crude aqueous and ethanol extract could be used as a source of natural antioxidants of R. nasutus and needs further studies to bring new natural products into pharmaceutical industries.

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References
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Journal ArticleDOI
TL;DR: The BGA team discusses the development of G-15, which aims to address the challenge of “superbugs” in the high-acid environment.
Abstract: グルコサミン塩酸塩を遊離形にし, 37℃インキュベーターで0日から30日間放置褐変した褐変グルコサミン (BGA) の抗酸化性, 還元力, 褐変度, アミノ糖の残存量, pH, 水分量, 全窒素量を, 放置0日から5日間は毎日, 以後5日間の間隔で30日間測定した。一方, 0, 15, 30日間放置褐変したBGAをセファデックスG-15で分画し, 抗酸化性, 還元力, 褐変度, pHについて測定して, 次のような結果を得た。1) 遊離グルコサミンは, 3日間放置後より白色粉末状から褐色ペースト状に急激な変化を示した。2) 最も強い抗酸化性は, 25日間と30日間放置褐変したBGAで認められた。3) BGAのリノール酸に対する抗酸化性は, 褐変度と深い関係を示した。4) 長く放置褐変したBGAは, 分子量が比較的高い領域の褐変生成物質と, 比較的低い領域の褐変生成物質に分画された。5) 長く放置褐変したBGAでは, 高分子の褐変生成物質のフラクションと, 低分子の褐変生成物質のフラクションの中間フラクションに抗酸化性を認めた。

6,976 citations


"In vitro antioxidant activity of aq..." refers methods in this paper

  • ...The reducing power performed according to the method of Oyaizu (7)....

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Journal ArticleDOI
TL;DR: In this paper, the autoxidation of soybean oil in a cyclodextrin emulsion system was studied in the presence of an emulsion stabilizer consisting of polysaccharides such as xanthan, tragacanth gum, and methylcellulose.
Abstract: The autoxidation of soybean oil in a cyclodextrin emulsion system was studied in the presence of an emulsion stabilizer consisting of polysaccharides such as xanthan, tragacanth gum, and methylcellulose. Xanthan strongly inhibited the peroxidation of soybean oil containing tocopherols but showed no antioxidant activity on soybean oil without tocopherols in the emulsion. Xanthan did not have hydrogen donating ability but expressed Fe2+-binding activity. The Fe2+-binding activity corresponded to the pyruvate content of xanthan. Depyruvated xanthan did not inhibit effectively the autoxidation of soybean oil. The Fe2+-chelating structure of xanthan is discussed

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Journal ArticleDOI
TL;DR: Of the compatible solutes tested, sorbitol, mannitol, myo-inositol and proline were effective hydroxyl radical scavengers and Glycinebetaine was ineffective.

1,969 citations


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  • ...Hydroxyl radical scavenging activity of plant extracts were assay by the method of (6)....

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Journal ArticleDOI
TL;DR: A rapid evaluation for antioxidants, using two TLC screening methods, showed that thymoquinone and the components carvacrol, t‐anethole and 4‐terpineol demonstrated respectable radical scavenging property.
Abstract: The essential oil of black cumin seeds, Nigella sativa L., was tested for a possible antioxidant activity. A rapid evaluation for antioxidants, using two TLC screening methods, showed that thymoquinone and the components carvacrol, t-anethole and 4-terpineol demonstrated respectable radical scavenging property. These four constituents and the essential oil possessed variable antioxidant activity when tested in the diphenylpicrylhydracyl assay for non-specific hydrogen atom or electron donating activity. They were also effective.OH radical scavenging agents in the assay for non-enzymatic lipid peroxidation in liposomes and the deoxyribose degradation assay. GC-MS analysis of the essential oil obtained from six different samples of Nigella sativa seeds and from a commercial fixed oil showed that the qualitative composition of the volatile compounds was almost identical. Differences were mainly restricted to the quantitative composition.

1,807 citations


"In vitro antioxidant activity of aq..." refers methods in this paper

  • ...DPPH antioxidant assay was based on the ability of the DPPH, a free radical are stable, which can be gets decolorized in the presence of antioxidants (8,9)....

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