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Journal ArticleDOI: 10.1080/24701556.2016.1241272

In vitro bactericidal activity of biosynthesized CuS nanoparticles against UTI-causing pathogens

04 Aug 2017-Inorganic and Nano-Metal Chemistry (Taylor & Francis)-Vol. 47, Iss: 9, pp 1290-1297
Abstract: Nanoparticles are playing a vital role in antimicrobials. The culture supernatant of Serratia nematodiphila is used for reduction of copper sulfate into copper sulfide nanoparticles (CuS-NPs). The present study includes characterization of CdS nanoparticles using UV–vis spectrophotometer, size and morphology by SEM and TEM, and compositional analysis by energy-dispersive X-ray analysis (EDX). The CuS nanoparticles are 9–20 nm in size, measured using EM imaging. The antibacterial activity of CuS nanoparticles was analyzed against urinary tract infection (UTI) pathogens such as Escherichia coli, Staphylococcus aureus, Proteus vulgaris, and Klebsiella pneumoniae. CuS-NPs were found to exhibit good bactericidal property against the UTI after completing successful clinical trials. more

Topics: Proteus vulgaris (50%)

Journal ArticleDOI: 10.1016/J.COLSURFB.2019.110622
Abstract: The bactericidal activity of copper and copper alloys is well appreciated and was already exploited in medical practice in 19th century. However, despite of being an essential nutrient required by organisms to perform life functions, excess copper is extremely toxic and detrimental to health. Recent studies have shown that superhydrophobic surfaces have a significant antibacterial potential for reduction of nosocomial infections. At the same time, the prolonged contact with biological liquids may cause a degradation of the superhydrophobic copper surface and corrosion with increasing egress of toxic copper ions. These aspects are poorly studied so far. In this paper, we analyze the evolution of the properties of both the superhydrophobic copper surface and the suspension of Escherichia coli bacteria during their prolonged contact and study the impact of such contact on the bactericidal activity of the surface. It is shown that by controlling the corrosion resistance and the wettability of the superhydrophobic copper substrate, it becomes possible to sustain the bactericidal action of copper substrates for a long time, simultaneously avoiding the excessive corrosive degradation and release of copper ions in the environment. more

Topics: Copper (55%)

22 Citations

Journal ArticleDOI: 10.1021/ACSSUSCHEMENG.9B01849
Zh. S. Shalabayev1, Matej Baláž2, Nina Daneu3, Erika Dutková2  +7 moreInstitutions (4)
Abstract: In the present study, a quick and environmentally friendly approach has been developed for the synthesis of CuS nanocrystals of different shape with antibacterial activity The process is completed more

Topics: Copper sulfide (52%), Sulfur (51%)

15 Citations

Journal ArticleDOI: 10.1007/S10971-019-05023-1
Abstract: In recent years, copper sulfide has attracted great interest in biological application, due to its biocompatibility and low toxicity. Undoped and nickel (Ni)-doped copper sulfide nanostructures were synthesized using a hydrothermal method at 150 °C for 15 h. XRD patterns show that all samples have confirmed the formation of a hexagonal phase. Absorbance spectra were measured by using a UV–vis spectrophotometer. The optical bandgap energy of CuS nanostructure decreased with increasing Ni doping concentration. Their optical bandgap energies were 3.2, 3.21, 2.9, and 2.9 eV for undoped and Ni-doped copper sulfide nanostructures with concentrations of 1, 3, and 5%, respectively. The antibacterial activity of CuS nanostructure against E. coli, P. aeruginosa, and S. aureus was evaluated by zone of inhibition. The test revealed that the minimum concentration of CuS nanostructure has a strong antibacterial activity against Gram-positive bacteria than for Gram-negative bacteria. Antitumor treatment was applied based on employing CuS nanostructures by exploiting their unique optical and morphological properties as therapeutic agents against rhabdomyosarcoma (RD) and murine fibroblast (L20B) cancer cell lines without using laser. The cytotoxicity effect was evaluated by MTT assay; the results demonstrated that CuS nanostructure with 1% Ni doping exhibits more toxicity effect than undoped and doped nanostructures, with 3 and 5% for all particle concentrations, where cytotoxicity reaches 34.3% at 0.125 mg/ml, being the most influential concentration, which could be a promising agent for cancer treatment. more

Topics: Nanostructure (53%), Copper sulfide (51%)

11 Citations

Open accessJournal ArticleDOI: 10.1016/J.SAJCE.2018.04.004
M.I. Niyas Ahamed1, Shanmugam Rajeshkumar2, V. Ragul1, S. Anand1  +1 moreInstitutions (2)
Abstract: Since its high toxicity and mobility, hexavalent chromium is considered to be a high priority pollutant. This study was performed to carry out a remediation test along with toxicity assessment in a water sample collected from the saturated zone of a historically Cr (VI)-contaminated site known as a Puliyanthangal lake (Tamilnadu, India) using nanoscale zero-valent iron (nZVI). The water samples were examined before and after the nZVI application by means of microbial cultivation tests, phospholipid fatty acid analysis (PLFA) and toxicological tests. The present experimental results revealed that Cr (VI) is considerably adsorbed on nZVI nanoparticles and it could be a cost-effective method for the in situ remediations of Cr (VI). In addition, standard plate count assay showed the dose-dependent decrease in the bacterial cell viability in lake water sample after the addition of nZVI. more

8 Citations

Book ChapterDOI: 10.1007/978-3-030-20283-5_24
02 Jul 2018-
Abstract: Copper nanoparticles (CuNPs) have different structural properties and effective biological activities. One of the major proven applications of CuNPs is antimicrobial activity. The advantage of CuNPs is the control over particle size and compositions to provide additional applications. The synthesis of CuNPs through green synthesis, chemical, physical and biological methods is possible. The toxicity and stability of CuNPs are important for its use as antimicrobial agent. This work reviews the behavior of CuNPs and copper-based NPs as antimicrobial agents in different media under various conditions. more

7 Citations


Journal ArticleDOI: 10.1016/J.CHEMOSPHERE.2007.11.047
01 Apr 2008-Chemosphere
Abstract: As the production of nanoparticles of ZnO, TiO2 and CuO is increasing, their (eco)toxicity to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus was studied with a special emphasis on product formulations (nano or bulk oxides) and solubilization of particles. Our innovative approach based on the combination of traditional ecotoxicology methods and metal-specific recombinant biosensors allowed to clearly differentiate the toxic effects of metal oxides per se and solubilized metal ions. Suspensions of nano and bulk TiO2 were not toxic even at 20 g l(-1). All Zn formulations were very toxic: L(E)C50 (mg l(-1)) for bulk ZnO, nanoZnO and ZnSO4.7H2O: 1.8, 1.9, 1.1 (V. fischeri); 8.8, 3.2, 6.1 (D. magna) and 0.24, 0.18, 0.98 (T. platyurus), respectively. The toxicity was due to solubilized Zn ions as proved with recombinant Zn-sensor bacteria. Differently from Zn compounds, Cu compounds had different toxicities: L(E)C50 (mg l(-1)) for bulk CuO, nano CuO and CuSO4: 3811, 79, 1.6 (V. fischeri), 165, 3.2, 0,17 (D. magna) and 95, 2.1, 0.11 (T. platyurus), respectively. Cu-sensor bacteria showed that toxicity to V. fischeri and T. platyurus was largely explained by soluble Cu ions. However, for Daphnia magna, nano and bulk CuO proved less bioavailable than for bacterial Cu-sensor. This is the first evaluation of ZnO, CuO and TiO2 toxicity to V. fischeri and T. platyurus. For nano ZnO and nano CuO this is also a first study for D. magna. more

Topics: Daphnia magna (54%), Antibacterial agent (51%)

1,279 Citations

Journal ArticleDOI: 10.1016/J.CIS.2010.02.001
Abstract: An array of physical, chemical and biological methods have been used to synthesize nanomaterials. In order to synthesize noble metal nanoparticles of particular shape and size specific methodologies have been formulated. Although ultraviolet irradiation, aerosol technologies, lithography, laser ablation, ultrasonic fields, and photochemical reduction techniques have been used successfully to produce nanoparticles, they remain expensive and involve the use of hazardous chemicals. Therefore, there is a growing concern to develop environment-friendly and sustainable methods. Since the synthesis of nanoparticles of different compositions, sizes, shapes and controlled dispersity is an important aspect of nanotechnology new cost-effective procedures are being developed. Microbial synthesis of nanoparticles is a green chemistry approach that interconnects nanotechnology and microbial biotechnology. Biosynthesis of gold, silver, gold-silver alloy, selenium, tellurium, platinum, palladium, silica, titania, zirconia, quantum dots, magnetite and uraninite nanoparticles by bacteria, actinomycetes, fungi, yeasts and viruses have been reported. However, despite the stability, biological nanoparticles are not monodispersed and the rate of synthesis is slow. To overcome these problems, several factors such as microbial cultivation methods and the extraction techniques have to be optimized and the combinatorial approach such as photobiological methods may be used. Cellular, biochemical and molecular mechanisms that mediate the synthesis of biological nanoparticles should be studied in detail to increase the rate of synthesis and improve properties of nanoparticles. Owing to the rich biodiversity of microbes, their potential as biological materials for nanoparticle synthesis is yet to be fully explored. In this review, we present the current status of microbial synthesis and applications of metal nanoparticles. more

Topics: Nanoparticle (52%)

1,241 Citations

Journal ArticleDOI: 10.1038/NRUROL.2010.190
Betsy Foxman1Institutions (1)
Abstract: Urinary tract infections (UTIs) are among the most common bacterial infections acquired in the community and in hospitals. In individuals without anatomical or functional abnormalities, UTIs are generally self limiting, but have a propensity to recur. Uropathogens have specialized characteristics, such as the production of adhesins, siderophores and toxins that enable them to colonize and invade the urinary tract, and are transmitted between individuals both through person-to-person contact and possibly via food or water. Although generally self limiting, treatment of UTIs with antibiotics leads to a more rapid resolution of symptoms and is more likely to clear bacteriuria, but also selects for resistant uropathogens and commensal bacteria and adversely affects the gut and vaginal microbiota. As uropathogens are increasingly becoming resistant to currently available antibiotics, it may be time to explore alternative strategies for managing UTI. more

Topics: Bacteriuria (55%)

1,057 Citations

Open accessJournal ArticleDOI: 10.1186/1477-3155-3-8
Abstract: Extracellular production of metal nanoparticles by several strains of the fungus Fusarium oxysporum was carried out It was found that aqueous silver ions when exposed to several Fusarium oxysporum strains are reduced in solution, thereby leading to the formation of silver hydrosol The silver nanoparticles were in the range of 20–50 nm in dimensions The reduction of the metal ions occurs by a nitrate-dependent reductase and a shuttle quinone extracellular process The potentialities of this nanotechnological design based in fugal biosynthesis of nanoparticles for several technical applications are important, including their high potential as antibacterial material more

Topics: Silver nanoparticle (61%), Fusarium oxysporum (58%)

904 Citations

Journal ArticleDOI: 10.7326/0003-4819-135-1-200107030-00012
Abstract: The authors highlight the problem of antimicrobial resistance in acute uncomplicated community-acquired urinary tract infection (UTI), summarize the few available data on clinical outcomes associat... more

638 Citations