In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride.
Citations
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Cites background from "In vitro cytotoxicity assays: compa..."
...Cellular toxicity refers to single cell toxicity, which can be measured for human cells using MTT assays, LDH release, and ATP synthesis (Fotakis and Timbrell, 2006)....
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...Cellular toxicity refers to single cell toxicity, which can be measured for human cells using MTT assays, LDH release, and ATP synthesis (Fotakis and Timbrell, 2006)....
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345 citations
Additional excerpts
...Cell enumeration independent of enzymati conversion of dye [30,31]...
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328 citations
Cites result from "In vitro cytotoxicity assays: compa..."
...These findings are perfectly in agreement with previous comparisons of various cell viability assays [24]....
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283 citations
Cites methods from "In vitro cytotoxicity assays: compa..."
...The discrepancy between, and limitations in, the assays have been noted previously (Lobner, 2000; Fotakis and Timbrell, 2006; Liu and Dalgleish, 2009) and could be because of the washing steps that are part of the MTT assay, whereas the LDH assay uses the culture medium directly from the cells....
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References
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"In vitro cytotoxicity assays: compa..." refers methods in this paper
...The MTT assay was tested for its validity in various cell lines (Mossmann, 1983)....
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...The MTT assay is based on the protocol described for the first time byMossmann (1983) ....
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...Further modification of the initial protocol described by Mossmann (1983)were proposed (Denizot and Lang, 1986; Hansen et al., 1989) in order to improve the repeatability and the sensitivity of the assay....
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4,631 citations
"In vitro cytotoxicity assays: compa..." refers methods in this paper
...Further modification of the initial protocol described by Mossmann (1983) were proposed ( Denizot and Lang, 1986; Hansen et al., 1989) in order to improve the repeatability and the sensitivity of the assay....
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...Further modification of the initial protocol described by Mossmann (1983)were proposed (Denizot and Lang, 1986; Hansen et al., 1989) in order to improve the repeatability and the sensitivity of the assay....
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3,442 citations
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"In vitro cytotoxicity assays: compa..." refers background in this paper
...More recent evidence suggests that reduction of MTT can also be mediated by NADH or NADPH within the cells and out of mitochondria (Berridge and Tan, 1992)....
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...Keywords: Cadmium; LDH; MTT; Neutral red; Cytotoxicity...
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