In vitro regeneration of Ruscus hypophyllum L. plants
01 Jan 1985-Plant Cell Tissue and Organ Culture (Martinus Nijhoff/Dr. W. Junk Publishers)-Vol. 5, Iss: 1, pp 79-87
TL;DR: Callus cultures were established from stem explants of Ruscus hypophyllum on a modified basal medium of Murashige and Skoog (1962) supplemented with 1 mg l-1 2,4-D+0.1 mgL-1 BAP and eighty percent of plants transferred to soil have survived.
Abstract: Callus cultures were established from stem explants of Ruscus hypophyllum on a modified basal medium of Murashige and Skoog (1962) supplemented with 1 mg l-1 2,4-D+0.1 mg l-1 BAP. The optimal 2,4-D concentration for promoting shoot bud formation and growth was 0.05 mg l-1 along with 0.5 mg l-1 BAP. Sixty percent of rootless shoots produced flowers on the regenerating medium. Rooting was induced when shoots were transferred to half strength MS inorganic salts supplemented with 2 mg l-1 IBA. Eighty percent of plants transferred to soil have survived.
TL;DR: Rapid propagation by the formation of shoots from calli of Aloe vera was obtained in the present investigation and callus formation was induced in stem segments from young axillary shoots grown on the underground rhizomatous stem.
Abstract: Rapid propagation by the formation of shoots from calli of Aloe vera was obtained in the present investigation. Callus formation was induced in stem segments from young axillary shoots grown on the underground rhizomatous stem. The use of polyvinylpyrrolidone (PVP) in the nutrient media reduced the secretion of phenolic substances from the explant. Murashige and Skoog's basal medium containing 1 mg l−1 2,4-dichlorophenoxyacetic acid and 0.2 mg l−1 kinetin gave the best callus induction. Shoots were initiated from the calli with reduced 2,4-D and increased kinetin concentration.
TL;DR: A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed and higher numbers of bulblets were obtained from bulb scale explants than embryo explants.
Abstract: A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed. Bulb scale, scape, leaf, and immature embryo explants were cultured on different nutrient media compositions supplemented with various concentrations of plant growth regulators (BAP, NAA, TDZ, Picloram, 2,4-D). Scape explants produced callus only. Higher numbers of bulblets were obtained from bulb scale explants than embryo explants. The highest percentage of the bulb formation (23.50 per explant) were obtained from bulb scale explants consisting of 4 scale segments cultured on Murashige and Skoog (MS) medium supplemented with 4 mg L-1 6-benzylaminopurine (BAP) and 0.25 mg L-1 a- naphthaleneacetic acid (NAA) after 5 months of culture initiation. Rooted bulblets which were more than 5 mm in diameter were transplanted into potting mixture of soil, vermiculite and perlite (1:1:1). The chromosome number of the root cells of in vitro induced bulblets was diploid 2n = 18.
Cites background from "In vitro regeneration of Ruscus hyp..."
01 Jan 2005
TL;DR: Efficient procedures are outlined for plant regeneration through direct shoot bud formation and indirect organogenesis through callus formation in Ornithogalum virens Lindl using bulb scale as explant using Murashige and Skoog's medium.
Abstract: Efficient procedures are outlined for plant regeneration through direct shoot bud formation and indirect organogenesis through callus formation in Ornithogalum virens Lindl using bulb scale as explant. Murashige and Skoog's (MS) medium containing 1 mg/L (5.4mM) NAA and 2 mg/L (4.4mM) BA was most effective in direct induction of shoot buds from explant. Callus cultures were raised from the bulb scale segment on MS medium supplemented with 2 mg/L (9.0mM) 2,4-D. Shoot regeneration from callus was optimum on the medium containing 2 mg/L (10.7mM) NAA and 0.5 mg/L (2.2mM) BA. Shoots developed roots on MS basal medium devoid of growth regulators. Regenerated plants grew profusely in MS liquid medium and were successfully transferred to pots. Bulblets were induced at the base of the regenerants upon transfer to MS basal medium supplemented with enhanced concentrations of sucrose (45 to 90 g/L). Direct induction of bulblets also occurred on the bulb scale grown on the MS media supplemented with 1 mg/L (5.4mM) NAA, 2 mg/L (8.9mM) BA and 60 g/L sucrose. Size of bulblets could be increased by decreasing the salt strength of MS basal medium to half. The effect of in vitro induced bulblet size on the ex vitro survival rate was also reported. Bulblets produced in vitro could be transplanted directly to potted soil. Chromosome analysis of direct explant-derived plants, callus-derived regenerates, and plants sprouted from in vitro-induced bulblets revealed only diploid cells with normal karyotypes comprising 2n = 6 chromosomes. List of abbreviations: List of abbreviations: List of abbreviations: List of abbreviations: List of abbreviations: NAA, a-naphthalene acetic acid; BA, 6 benzyladenine; 2,4-D, 2,4 dichlorophenoxyacetic acid.
TL;DR: A standard protocol for rapid propagation of Asparagus robustus Hort.
Abstract: A standard protocol for rapid propagation of Asparagus robustus Hort. from callus has been developed. Callusing and regeneration were maximum using segments of shoot tissue. Shoot and internode tissue retained the ability to form callus with high regenerability in culture for 34 months. Basal medium supplemented with 3 mg/liter 2,4-D and 1 mg/liter Kn were most effective in formation of callus. Shoot formation was optimum on medium containing 0.1 mg/liter NAA, 1 mg/liter BAP, and 40 mg/liter adenine sulfate. Root induction was maximized in half-strength MS basal medium with 0.5 mg/liter IBA. The number and length of roots was greater under an 8 h photoperiod as compared with a 16 h photoperiod. Regenerated plants could be maintained in half-strength MS liquid medium for 30 days prior to transfer to potted soil.
TL;DR: In vitro regeneration spear, shoot tip, internode, node and root of mature plants of Asparagus cooperi were used as explants and Regenerated plants were cytologically and phenotypically stable.
Abstract: For in vitro regeneration spear, shoot tip, internode, node and root of mature plants of Asparagus cooperi were used as explants. Induction of callus from different explants depended on the photoperiod in addition to a specific combination of α-napthalene acetic acid (NAA) and kinetin (Kn) in the basal medium. The development of shoots from callus required 6-benzylaminopurine (BA), l -arginine, adenine and a low level of NAA. The individual shoots produced roots in the presence of indole-3-butyric acid (IBA) or indole-3-butyric acid containing potassium salt (KIBA). Regenerated plants were cytologically and phenotypically stable.
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the
01 May 1984
TL;DR: The author’s laboratory requirements and general techniques, as well as some of the techniques used, can be found in this monograph, which was written at the request of the author.
Abstract: 1. Introductory History. 2. Laboratory Requirements and General Techniques. 3. Tissue Culture Media. 4. Cell Culture. 5. Cellular Totipotency. 6. Somatic Embryogenesis. 7. Haploid Production. 8. Triploid Production. 9. Cytogenetic Studies. 10. In Vitro Pollination. 11. Zygotic Embryo Culture. 12. Protoplast Isolation and Culture. 13. Somatic Hybridization. 14. Production of Pathogen-Free Plants. 15. Clonal Propagation. 16. Germplasm Storage. References. Indexes.
TL;DR: This communication reports the successful establishment in both agar and liquid shake cultures of callus of Lilium longiflorum Thunb and the repeated subculturing of the callus on growth factor free media, and the production of large numbers of plantlets from callus under certain growth conditions in liquid suspension cultures.
Abstract: The monocot Lilium, possessing very large nuclei and chromosomes, is a favorable material for studying problems of development, parti cularly with regard to the proteins of the nucleus (Sheridan and Stern, 1967). Since it would broaden the possible approaches to such study if successful, an attempt was made to establish Lilium in tissue culture. This communication reports (1) the successful establishment in both agar and liquid shake cultures of callus of Lilium longiflorum Thunb ; (2) the repeated subculturing of the callus on growth factor free media; and (3) the production of large numbers of plantlets from callus under certain growth conditions in liquid suspension cultures. Callus was established by placing tissue expiants from steBi apices onto the basal medium supplemented with 2 mg/1 of indoleacetic acid (IAA). The terminal 10 cm of stems which were approximately 25 cm in height were removed and all but the smallest leaves were stripped off. The apices were washed for 5 min in a solution of the detergent Heikol, 10 min in 10% Clorox, and 5 min in sterile distilled water. Each apex was then placed in a sterile petri dish and the outer portion in cluding all the leaves and primordia was removed with a sc^pel leaving a cylinder which was free of any surface tissue. The terminal 2 cm were removed and placed on the medium. The basal medium was that of Linsmaier and Skoog (1965) con taining major and minor salts, organic supplements of thiamin and inositol, and 40 g of sucrose per liter. Basal agar medium refers to the above medium solidified with 0.8% agar. Fresh weight was used for determining growth response. Within three weeks of explantation, callus appeared on the edge of the tissue block in contact with the medium. The presence of IAA in the culture medium stimulated the production of callus by the stem tissue but was not essential for it. When expiants were placed on medium lacking IAA, buds appeared on the surface of the tissue blocks and shoots and roots were formed producing new plants; on occasion callus was also formed, but usually only after the formation of roots and shoots.