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Journal ArticleDOI

In vitro response as a reflection of genomic diversity in long-term cultures of Musa

01 Nov 1988-Theoretical and Applied Genetics (Theor Appl Genet)-Vol. 76, Iss: 5, pp 733-736
TL;DR: The regenerative potential of all cultivars of Musa, irrespective of their genomes, remained unaffected in long-term culture, even after 28–30 months, and genomic influence on both the nature and rate of proliferative growth was evident.
Abstract: Ten commercial cultivars of Musa representing five different types of genomic constitutions were studied for in vitro multiplication through meristem culture In addition, the effects of various genomic constitutions at different ploidy levels on growth and meristem proliferation in long-term cultures were analysed statistically Plantlets were readily obtained by culturing the excised meristems on MS semisolid medium supplemented with IAA, IBA and BAP at various concentrations The regenerative potential of all cultivars of Musa, irrespective of their genomes, remained unaffected in long-term culture, even after 28–30 months The genomic influence on both the nature and rate of proliferative growth was evident Statistical analysis revealed that the rates of meristem proliferation between different cultivars of the same passage and between different passages of the same cultivar were significantly different Those cultivars having only an A genome showed a low rate of meristem proliferation, while under the same culture conditions, cultivars having one or two B genomes in addition to the A exhibited a very high rate
Citations
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Book ChapterDOI
01 Jan 1991
TL;DR: Recent advances in molecular genetics, as well as more detailed physiological and cytological study of the effect of tissue culture on genotypic stability and plant performance, has allowed a clearer understanding of the observed variation.
Abstract: The occurrence of off-type plants propagated from tissue culture has caused concern among nurserymen (Anonymous, 1989; Evans et al., 1986; Knuttel, 1989; Read & Hosier, 1986; Stimart, 1986; Timmis & Ritchie, 1988; van Aartrijk & van der Linde, 1986). Typical off-type plants include dwarfs, color changes or mosaic patterns (e.g. chlorosis, loss of chimeras), growth habit changes (e.g. erectness, leaf shape, vigor), and changes in productivity (e.g. sterility, prolonged juvenility). Nevertheless, large numbers of plants are produced in vitro each year and typically variation among tissueculture-propagated plants from organized meristems is less than or equal to that observed from standard propagules (Ahmed et al., 1987; Ettinger & Preece, 1985; Hutchinson & Zimmerman, 1987; Jones 1988; Read & Hosier, 1986; Swartz & Lindstrom, 1986; Wu & Jampates, 1986). Recent advances in molecular genetics, as well as more detailed physiological and cytological study of the effect of tissue culture on genotypic stability and plant performance, has allowed a clearer understanding of the observed variation.

44 citations

Journal ArticleDOI
TL;DR: In vitro preservation techniques have allowed conservation of plant genetic resources for further use in plant breeding, research and food security purposes, mainly for those that are difficult to preserve conventionally.
Abstract: The aim of this literature review is to describethe main techniques for short and long term germplasmconservation. These techniques allow establishment ofgermplasm banks. This article focuses on their usein tropical crops. In vitro preservation techniqueshave allowed conservation of plant genetic resourcesfor further use in plant breeding, research and foodsecurity purposes. Several research centers have usedthese techniques for tissue and seed storage of tropicalcrops, mainly for those that are difficult to preserve conventionally.

11 citations

Journal ArticleDOI
30 May 2018
TL;DR: The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
Abstract: The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.

9 citations


Cites background from "In vitro response as a reflection o..."

  • ...1% mercuric chloride solution for five minutes was found to be effective sterilant for aseptic culture establishment of the cultivar as reported in many other in vitro cultures of banana [13, 14]....

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Journal ArticleDOI
TL;DR: The impact of accessions in Centella asiatica is discussed and what more need to be done to the authors' knowledge of accession and the reasons for variations in chemical profiling of secondary metabolites are discussed.
Abstract: Centella asiatica is described as a potential cure-all because of its wide usage. The accessions in Centella asiatica make standardisation important before using it. In Malaysia alone, there are more than 15 accessions of Centella asiatica with each having variation in their secondary metabolites. There are several reports from India to Korea, from Madagascar to Malaysia and from Thailand to South Africa about variations in chemical profiling of secondary metabolites and the reasons for these variations in Centella asiatica. Despite all these reports, there has been no attempt to bring all these variations together and discuss them under one heading resulting into scattered information about accession in Centella asiatica. In this review, we discuss the impact of accessions in Centella asiatica and what more need to be done to our knowledge of accession in Centella asiatica.

7 citations


Cites background from "In vitro response as a reflection o..."

  • ...International Food Research Journal 2011; 18(4): 1215-1222 20....

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  • ...Acta Botanica Gallica 1997; 144 (4): 489-493....

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  • ...J PURE APPL MICROBIO, 10(4), DECEMBER 2016....

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Journal ArticleDOI
TL;DR: An experiment has been designed to develop a suitable and efficient protocol for plant regeneration in banana through micro propagation by optimizing the concentration and duration of different sterilants for explants surface sterilization.
Abstract: Production of Banana is constrained by many biotic as well as abiotic factors. The production of disease free planting material of banana by meristem culture technique is an urgent need.An experiment has been designed to develop a suitable and efficient protocol for plant regeneration in banana through micro propagation.The surface sterilization of banana plant is the most difficult practice.So it is very important to optimize the concentration and duration of different sterilants for explants surface sterilization. The best sterilants for the surface sterilization of the explant of banana were 0.1 per cent HgCl 2 for 5 min duration followed by NaOCl (40 %, commercially available) solution for 8 min duration and 70 per cent ethyl alcohol for 2 min. And the best medium for establishment of banana explant was supplemented with 3mg/l BAP, 0.2mg/l IAA with 30 mg ascorbic acid followed by Proliferation with 5mg/l BAP,0.5mg/ l IAA with 30 mg ascorbic acid and 10 mg/l BAP,0.5 mg/l IAA with 30 mg ascorbic acid.Significantly highest number of roots and greater root length were produced by 0.5 mg/l IAA + 0.5 mg/l IBA. How to view point the article : Kumari, Namrata and Misra, Pragati (2016). Mass in-vitro micro propagation of banana (Musa sp.). Internat. J. Plant Protec., 9(1) : 204-210.

4 citations

References
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Journal ArticleDOI
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the

63,098 citations


"In vitro response as a reflection o..." refers methods in this paper

  • ...Explants were cultured on MS basal medium (Murashige and Skoog 1962) supplemented with various growth regulators at different concentrations....

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Book
01 Jan 1973
TL;DR: In this article, the authors introduce the concept of biostatistics and introduce the Biostatistic Approach to Biometrics (BAPB) as a method for biometrics.
Abstract: Introduction to Biostatistics , Introduction to Biostatistics , کتابخانه مرکزی دانشگاه علوم پزشکی تهران

1,874 citations

Book
01 Oct 1959
TL;DR: In this article, the authors report critical values (to four significant figures) which have been found by inverse interpolation in the new table of the probability integral of the studentized range.
Abstract: Abstract : The purpose of this paper is to report critical values (to four significant figures) which have been found by inverse interpolation in the new table of the probability integral of the studentized range. Included are corrected tables for significance levels alpha = 0.05, 0.1 and new tables for significance levels alpha = 0.10, 0.005, 0.001-all with sample sizes n = 2(1) 20(2)40(10)100 and degrees of freedom nu = 1(1)20, 24, 30, 60, 120, infinity.

614 citations

Journal ArticleDOI
TL;DR: In this paper, the authors report critical values (to four significant figures) which have been found by inverse interpolation in the new table of the probability integral of the studentized range.
Abstract: : The purpose of this paper is to report critical values (to four significant figures) which have been found by inverse interpolation in the new table of the probability integral of the studentized range. Included are corrected tables for significance levels alpha = 0.05, 0.1 and new tables for significance levels alpha = 0.10, 0.005, 0.001-all with sample sizes n = 2(1) 20(2)40(10)100 and degrees of freedom nu = 1(1)20, 24, 30, 60, 120, infinity.

579 citations

Journal ArticleDOI
TL;DR: A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions is presented and the cultivars tested seem to vary in their ability to withstand minimal growth temperature.
Abstract: A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions is presented in this paper Shoot-tip cultures of Musa cultivars (both banana and plantain) are induced by culturing small excised shoot apices on modified MS semisolid medium supplemented with various concentrations and combinations of auxins and cytokinins The effects of cytokinin concentration in the medium as well as the genotypic configuration of the cultivars on the rate of shoot-bud proliferation have been tested The established shoot-tip cultures grown on modified MS semisolid medium supplemented with IAA (018 mg/l) and BA (230 mg/l) have been successfully stored at 15°C with 1000 lux light intensity up to 13–17 months depending on the cultivar The cultivars tested in the present investigation seem to vary in their ability to withstand minimal growth temperature

186 citations